基于化学计量法对荧光光谱重叠的维生素B1、B2和B6的测定

薛永林; 高玲; 任守信

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基于化学计量法对荧光光谱重叠的维生素B₁、B₂和B₆的测定

光谱分析 | 更新时间：2020-08-12

- 基于化学计量法对荧光光谱重叠的维生素B₁、B₂和B₆的测定
- Determination Based on Chemometrics Spectral Geminated Vitamin B₁, B₂ and B₆
- 发光学报 2011年32卷第10期页码：1081-1087
- 作者机构：
  
  内蒙古大学化学化工学院,内蒙古呼和浩特,010021
- 作者简介：
- 基金信息：
  
  国家自然科学基金(21067006,60762003)();内蒙古自然科学基金(2009MS0209)资助项目()
- DOI：
  中图分类号： O657.3
- 纸质出版日期：2011-10-22，
  
  网络出版日期：2011-10-22，
  
  收稿日期：2011-4-1，
  
  修回日期：2011-6-16，
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薛永林, 高玲, 任守信. 基于化学计量法对荧光光谱重叠的维生素B1、B2和B6的测定[J]. 发光学报, 2011,32(10): 1081-1087

XUE Yong-Lin, GAO Ling, REN Shou-xin. Determination Based on Chemometrics Spectral Geminated Vitamin B1, B2 and B6[J]. Chinese Journal of Luminescence, 2011,32(10): 1081-1087
薛永林, 高玲, 任守信. 基于化学计量法对荧光光谱重叠的维生素B1、B2和B6的测定[J]. 发光学报, 2011,32(10): 1081-1087 DOI：

XUE Yong-Lin, GAO Ling, REN Shou-xin. Determination Based on Chemometrics Spectral Geminated Vitamin B1, B2 and B6[J]. Chinese Journal of Luminescence, 2011,32(10): 1081-1087 DOI：

摘要

利用直接正交信号校正(DOSC)-小波包变换(WPT)-偏最小二乘法(PLS)(DOSC-WPT-PLS)新方法对荧光光谱严重重叠的维生素B

１

、B

２

和B

６

三组分混合体系进行了同时测定。设计了PDOSCWPTPLS程序执行相关计算

并将三种化学计量学方法(DOSC-WPT-PLS、WPT-PLS和PLS)进行了比较。三种维生素B的总体相对预测标准偏差分别为2.29%、4.23%和5.36%。结果表明DOSC-WPT-PLS法是成功的且优于WPT-PLS法和PLS法。将该法用于测定自来水中的维生素B

１

、B

２

和B

６

的含量其回收率分别为93.4%~109.2%、91.0%~105.2%、94.7%~107.7%

获得了满意的结果。

Abstract

A DOSC-WPT-PLS method based on partial least squares (PLS) regression with direct orthogonal signal correction (DOSC) and wavelet packet transform (WPT) as preprocessed tools was proposed for the simultaneous fluorespectrophotometric determination of Vitamin B

vitamin B

and vitamin B

. A program (PDOSCWPTPLS) was designed to execute the relative calculations. Three chemometric methods (DOSC-WPT-PLS

WPT-PLS and PLS) were compared in this study. The relative standard errors of prediction (RSEP) obtained for all components with DOSC-WPT-PLS

WPT-PLS and PLS were 2.29%

4.23% and 5.36%

respectively. Experimental results showed that the DOSC-WPT-PLS method was successful and had advantages over the WPT-PLS and PLS. The method was applied to determine the contents of vitamin B

vitamin B

and vitamin B

in the tap water with satisfactory results and their recoveries were 93.4%~109.2%

91.0%~105.2% and 94.7%~107.7%

respectively.

关键词

化学计量学荧光光度法同时测定维生素B

Keywords

chemometricfluorespectrophotometrysimultaneous determinationvitamin B

references

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