环丙沙星、氧氟沙星同时 与牛血清白蛋白分子作用的荧光光谱

刘保生; 薛春丽; 王 晶; 杨 超; 吕运开

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环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱

论文 | 更新时间：2020-08-12

- 环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱
- Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods
- 发光学报 2010年31卷第2期页码：285-290
- 作者机构：
  
  河北大学理化分析中心药物化学与分子诊断教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： O657.3;O482.31
- 收稿：2009-11-25，
  
  修回：1900-1-2，
  
  网络首发：2010-04-30，
  
  纸质出版：2010-04-30
- 稿件说明：
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刘保生, 薛春丽, 王晶, 等. 环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱[J]. 发光学报, 2010,31(2):285-290.

LIU Bao-sheng, XUE Chun-li, WANG Jing, et al. Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods[J]. Chinese Journal of Luminescence, 2010, 31(2): 285-290.
刘保生, 薛春丽, 王晶, 等. 环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱[J]. 发光学报, 2010,31(2):285-290. DOI：

LIU Bao-sheng, XUE Chun-li, WANG Jing, et al. Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods[J]. Chinese Journal of Luminescence, 2010, 31(2): 285-290. DOI：

摘要

在pH=7.40的水溶液中

环丙沙星(CPFX)、氧氟沙星(OFLX)能够猝灭牛血清白蛋白(BSA)的荧光。当两种药物共存时BSA荧光被进一步猝灭。据此建立了利用荧光发光光谱法进行喹诺酮类药物CPFX与OFLX间相互作用的研究。结果表明:药物间存在相互作用

使药物与蛋白间的结合常数减小、结合稳定性下降

游离型药物含量增加

造成药效增强;药物对蛋白荧光的猝灭属于静态猝灭

药物与蛋白结合位点数约为1。根据Frster非辐射能量转移理论

确定了药物与蛋白之间的结合距离

＜7 nm

属于非辐射能量转移。药物间的相互作

用使r

值增加

结合距离增大。同步荧光光谱研究表明药物间的相互作用对蛋白构象产生影响

使蛋白质分子伸展

疏水性降低。

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. Shao Shuang, Ma Boying, Wang Xuejie, et al. Study on the interaction between cefodium sodium and serum albumin [J]. Acta Phys. Chim. Sin. (物理化学学报), 2005, 21 (7):792-795 (in Chinese).

. Mathias U, Jung M. Determination of drug-serum protein interactions via fluorescence polarization measurements [J]. Anal. Bioanal. Chem., 2007, 388 (5-6):1147-1156.

. Rus O B, Puchkaev A V, Ivanov A I, et al. Interaction of albumins and hemin with aromatic antioxidants: A spectrophotometric and fluorometric study [J]. Appl. Biochem. Micro., 2006, 36 (1):36-45.

. Smyk B. Fluorescence study of sinapic acid interaction with bovine serum albumin and egg albumin [J]. J. Fluoresc., 2003, 13 (4):349-356.

. Bai Haixin, Yang Cheng, Yang Xiurong. Interaction between bovine serum albumin and Indo-1 using fluorescence spectroscopic method [J]. Chem. J. Chinese Univ. (高等学校化学学报), 2007, 28 (2):227-332 (in Chinese).

. Feng Xizeng, Bai Chunli, Lin Zhang.The interaction between acridine orange and bovine serum albumin [J]. Anal.Chem. (分析化学), 1998, 26 (2):154-157 (in Chinese).

. Long Xiufen, Liu Shaopu, Kong Ling, et al. A study on the interaction of proteins with some heteropoly compounds and their analytical application by resonance Rayleigh scattering method [J]. Talanta, 2004, 63 (2):279-286.

. Gong Aiqin, Zhu Xiashi, Hu Yanyan, et al. A fluorescence spectroscopic study of the interaction between epristeride and bovine serum albumin and its analytical application [J]. Talanta, 2007, 73 (4):668-673.

. Zhang Qingzhu. Fundamental Pharmacology [M]. Beijing: Higher Education Press, 2006, 48.

. Zhou Xiumei, Yang Wenbin, Chai Qiuyan. Studies of general pharmacological experiment on danshen injection [J]. Shanxi Med. J. (山西医药学杂志), 2004, 33 (12):1028-1030 (in Chinese).

. Li Xin, Gong Zehui, Chi Mugen, et al. Screening of ET-1receptor antagonists and the pharmacological evaluation [J]. Chin. Pharmacological Bulletin. (中国药理学通报), 2008, 24 (11):1422-1426 (in Chinese).

. Zhang Xiaowei, Zhao Fenglin, Li Kean. Studies on the reaction between ciprofloxacin and bovine serum albumin [J]. Chem. J. Chin. Univ. (高等学校化学学报), 1999, 20 (7):1063-1067 (in Chinese).

. Zou Jun,Li Xinxin,Yang Zhaoxu, et al. In vitroactivities of levofloxacin against helicobacter pylori [J]. J. Chin. Anti-biotics. (中国抗生素杂志), 2003, 28 (5):292-294 (in Chinese).

. Zhang Hongmei, Wang Yangqing, Yu Liming, et al. Spectroscopic study on interaction between zinc phthalocya-nine and bovine hemoglobin (BHb) [J]. Chemical Reagents (化学试剂), 2008, 30 (2):85-88 (in Chinese).

. Wei Yanli, Li Jianqing, Dong Chuan, et al. Investigation of the association behaviors between biliverdin and bovine serum albumin by fluorescence spectroscopy [J]. Talanta, 2006, 70 (2):377-382.

. Sun Yantao, Zhang Haitao, Sun Ye, et al. Study of interaction between protein and mainactive components in citrus aurantium L. by optical spectroscopy [J]. J. Lumin., 2010, 130 (2):270-279.

. Jiang Zhiqiang, Chi Yanhua, Zhuang Jia, et al. Mechanism studies on the combination Reaction between bovine serum albumin and zincon by fluorescence spectra [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2007, 27 (5):986-990 (in Chinese).

. Pu Yingqiu, Tao Weibo. Studies on the interaction between seratrodastand bovine serum album in by fluorescence spectroscop [J]. Phamaceutical and Clinical Research (药学与临床研究), 2007, 15 (4):287-291 (in Chinese).

. Ge Feng, Wang Jianping, Liu Diqiu, et al. Study on interaction between shikonin and bovine serum albumin by fluorescence spectra [J]. The Chinese Journal of Process Engineering (过程工程学报), 2009, 9 (1):118-122 (in Chinese).

. Huang Bo, Zou Guolin, Yang Tianming. Studies on the interaction between adriamycin and bovine serum albumin [J]. Acta Chimica Sinica (化学学报), 2002, 60 (10):1867-1871 (in Chinese).

. Wu Xiaohong, Zhou Jiahong, Gu Xiaotian, et al. Study on interaction between hypocrellin A and hemoglobin or myoglobin using synchronous fluorescence spectra [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2006, 26 (12): 2287-2290 (in Chinese).

. Chen Daiwu, Xie Qingji, Jiang Xueqin, et al. Inreraction of quercetion with casein and bovine serum albumin as well as effects of coexisting carbon nanotubes [J]. Acta Phys. Chim. Sin. (物理化学学报), 2008, 24 (3):379-387 (in Chinese).

. Yan Chengnong, Zhang Huaxin, Liu Yi, et al. Fluorescence spectra of the binding reaction between paraquat and bovine serum albumin [J]. Acta Chimica Sinica (化学学报), 2005, 63 (18):1727-1732 (in Chinese).

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