生物偶联过程对于聚合物包覆的水溶性CdSe/ZnS核壳量子点发光的影响

张友林; 曾庆辉; 孔祥贵

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生物偶联过程对于聚合物包覆的水溶性CdSe/ZnS核壳量子点发光的影响

论文 | 更新时间：2020-08-12

- 生物偶联过程对于聚合物包覆的水溶性CdSe/ZnS核壳量子点发光的影响
- The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer
- 发光学报 2010年31卷第1期页码：101-104
- 作者机构：
  
  1. 中国科学院研究生院北京,100049
  2. 中国科学院长春光学精密机械与物理研究所激发态物理重点实验室, 吉林长春 130033
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： O482.31
- 收稿日期：2009-05-08，
  
  修回日期：1900-01-02，
  
  网络出版日期：2010-02-20，
  
  纸质出版日期：2010-02-20
- 稿件说明：
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张友林, 曾庆辉, 孔祥贵. 生物偶联过程对于聚合物包覆的水溶性CdSe/ZnS核壳量子点发光的影响[J]. 发光学报, 2010,31(1):101-104.

ZHANG You-lin, ZENG Qing-hui, KONG Xiang-gui. The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer[J]. Chinese journal of luminescence, 2010, 31(1): 101-104.
张友林, 曾庆辉, 孔祥贵. 生物偶联过程对于聚合物包覆的水溶性CdSe/ZnS核壳量子点发光的影响[J]. 发光学报, 2010,31(1):101-104. DOI：

ZHANG You-lin, ZENG Qing-hui, KONG Xiang-gui. The Influence of Bioconjugate Process on the Photoluminescence Properties of Water-soluble CdSe/ZnS Core-shell Quantum Dots Capped with Polymer[J]. Chinese journal of luminescence, 2010, 31(1): 101-104. DOI：

摘要

以传统的戊二醛交联的方法实现了聚丙烯酸包覆的CdSe/ZnS量子点和人IgG蛋白分子的偶联

同时研究了偶联过程对量子点发光性能的影响。通过琼脂糖凝胶电泳技术证明量子点和蛋白分子偶联成功。通过稳态光谱和时间分辨光谱研究了偶联过程对量子点荧光性质的影响。发现偶联到量子点表面的戊二醛分子能够破坏量子点的表面从而增加其表面缺陷

使量子点发光效率降低;上述产物进一步与人IgG偶联增强了量子点的荧光强度

这是由于连接到量子点上的蛋白分子修复了量子点的表面

从而降低了表面缺陷所致。

Abstract

Conjugates of human IgG and CdSe/ZnS quantum dots (QDs) capped with an amphiphilic poly (acrylic acid) polymer modified with poly (ehylene glycol) amine were synthesized by a glutaraldehyde (Cho) cross-linking procedure. The IgG-QD assemblies form in this reaction

as evidenced by gel electrophoresis. The key function of QD bioconjugates is to fluoresce readily and remain stable in biological conditions in order to allow for efficient bioapplication. However

the photoluminescence (PL) properties of QDs are sensitive to their environment

so it is particular important to study the mechanisms of the PL change of QDs linked to biomolecules. The effect of cross-linking processes on the PL of QDs was studied by means of steady-state and time-resolved photoluminescence spectroscopy. Attachment of Cho on the surface of QDs results in a significant decrease of QD emission due to the increase of surface defects with Cho destroying the surface of QDs. Attachment of IgG on the surface of QDs results in a significant increase of QD-Cho emission

which is attributed to the conjugation of IgG to QD modifying the surface of CdSe/ZnS QDs.

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references

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