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1. 华东师范大学, 生命科学学院
2. 上海交通大学, 植物科学系
收稿:2007-09-13,
修回:2007-12-28,
纸质出版:2008-03-20
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袁萌芽, 陈季武, 陆泉枝, 姚雷, 方献平. 香叶天竺葵叶非精油组分清除自由基、抗氧化作用[J]. 发光学报, 2008,29(2): 381-386
YUAN Meng-ya, CHEN Ji-wu, LU Quan-zhi, YAO Lei, FANG Xian-ping. Study on Scavenging Free Radical and Antioxidation Effects of Nonessential Oil Extracts from Pelargonium Graveolens Leaves[J]. Chinese Journal of Luminescence, 2008,29(2): 381-386
袁萌芽, 陈季武, 陆泉枝, 姚雷, 方献平. 香叶天竺葵叶非精油组分清除自由基、抗氧化作用[J]. 发光学报, 2008,29(2): 381-386 DOI:
YUAN Meng-ya, CHEN Ji-wu, LU Quan-zhi, YAO Lei, FANG Xian-ping. Study on Scavenging Free Radical and Antioxidation Effects of Nonessential Oil Extracts from Pelargonium Graveolens Leaves[J]. Chinese Journal of Luminescence, 2008,29(2): 381-386 DOI:
活性氧与许多疾病的发生和发展密切相关
与食品的氧化也息息相关。所以筛选天然抗氧化剂显得非常重要。因此
分别采用水、乙醇和乙酸乙酯提取法从提取精油后的残渣中提取香叶天竺葵叶非精油有效组分
并分别采用超氧阴离子自由基(O
2
-·
)
羟自由基(
·
OH)
过氧亚硝基(ONOO
-
)和
·
OH氧化损伤DNA的四种化学发光体系和二苯代苦味肼基自由基(DPPH
&
#183;)和脂质过氧化两种比色体系检测香叶天竺葵叶非精油组分清除多种自由基、活性氧的能力以及对DNA的保护作用。结果表明
香叶天竺葵叶非精油组分的3种不同提取物均能有效地清除O
2
-·
、
·
OH、DPPH
·
和ONOO
-
减轻或消除
·
OH对DNA的氧化损伤
抑制脂质过氧化
是一种有效的、多功能的天然抗氧化剂和自由基清除剂。由于非精油组分是从制取精油组分后的残渣中提取的
提取装置和工艺也非常简单
故本研究不仅为香叶天竺葵种植效益的提高和综合利用提供了可能
也为天然香料加工后废弃物的开发研究提供了资料
对经济环境保护和资源的再利用也有借鉴意义。目前国内外对芳香植物的研究与开发主要聚焦于精油组分
而研究表明
更多的抗氧化物质是存在于非精油组分中
由于非精油组分是从制取精油组分后的残渣中提取的
并且提取装置和工艺简单
是值得进一步开发利用的天然产物。
Zhang C R,Song M,Zhang F F,et al.Study on tissue culture and high frequency propagation of pelargonium graveolens[J].J.Northwest Forestry University (西北林学院学报),2003,18(2):40-41 (in Chinese).
Sun W,Qu W J.Studys on pelargonium graveolens pharmacology research progress[J].J.Chin.Medicinal Materials(中药材学报),2002,25(8):600-602 (in Chinese).
Qin H Y,Chen J W,Hu B,et al.Study on scavenging free radical and antioxidation effects of extracts from salvia officinalis leaves[J].Food Science (食品科学),2006,27(7):89-92 (in Chinese).
Gao Y H,Huo G H,He X L,et al.Antioxidant activity study on Chinese tallow leaves(sapium sebiferum)extracts in vitro[J].Food Science (食品科学),2003,24(6):141-145 (in Chinese).
Chang C R.Ethyl acetate chemical reflux extraction and quantity determination of flavonoids in the Chinese quince[J].J.Guiyang Medical College (贵阳医学院学报),2001,26(4):326-327 (in Chinese).
Guo G G,Wang Z Y.Autoxidation of pyrogallol-chemiluminescence assay for superoxide dismutase activity[J].Plant Physiology Communications (植物生理学通讯),1989,(3):54-57 (in Chinese).
Xu S H.A new chemiluminescence system for measuring · OOH radical[J].J.Instrumental Analysis (分析测试学报),2000,19(2):11-13 (in Chinese).
Ma W J,Cao E H,Zhang J.The analysis of molecular structures and quantum mechanical calculations on the protection effect of several natural antioxidants against DNA damage[J].Acta Biophysica Sinica (生物物理学报),1998,14(1):155-160 (in Chinese).
Zhang E X,Yu L J,Zhou Y L,et al.Studies on the peroxidation of polyunsaturated fatty acid from lipoprotein induced by iron and the evaluation of the anti-oxidative activity of some natural products[J].Acta Biochimica et Biophysica Sinica (生物化学与生物物理学报),1996,28(2):218-222 (in Chinese).
Chen J W,Hu B,Su Y,et al.A novel chemiluminescence system for producing and measuring peroxynitrite anions[J].J.Radiat.Res.Radiat.Process (辐射研究与辐射工艺学报),2006,24(1):11-14 (in Chinese).
Jitesh S Rathee,Shyam A Hassarajani,Subrata Chattopadhyay.Antioxidant activity of mammea longifolia bud extracts[J].Food Chem.,2006,99(3):436-443.
Dumay A,Rincheval V,Trotot P,et al.The superoxide dismutase inhibitor diethyldithiocarbamate has antagonistic effects on apoptosis by triggering both cytochrome C release and caspase inhibition[J].Free Radical Biology & Medicine,2006,40(8):1377-1390.
Guidarelli A,Sciorati C,Clementi E,et al.Peroxynitrite mobilizes calcium ions from ryanodine-sensitive stores,a process associated with the mitochondrial accumulation of the cation and the enforced formation of species mediating cleavage of genomic DNA[J].Free Radical Biology & Medicine,2006,41(1):154-164.
Marnett L J.Oxy radicals,lipid peroxidation and DNA damage[J].Toxicology,2002,181-182:219-222.
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