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1. 湖北大学, 有机功能分子合成与应用教育部重点实验室
2. 孝感学院, 化学系
收稿:2007-07-19,
修回:2007-12-28,
纸质出版:2008-03-20
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周丽荣, 周立群, 余国锋, 唐子威, 王芬. SiO<sub>2</sub>包覆铕(Ⅲ)配合物的荧光纳米粒子合成与性质[J]. 发光学报, 2008,29(2): 371-375
ZHOU Li-rong, ZHOU Li-qun, YU Guo-feng, TANG Zi-wei, WANG Fen. Preparation and Properties of SiO<sub>2</sub> Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles[J]. Chinese Journal of Luminescence, 2008,29(2): 371-375
周丽荣, 周立群, 余国锋, 唐子威, 王芬. SiO<sub>2</sub>包覆铕(Ⅲ)配合物的荧光纳米粒子合成与性质[J]. 发光学报, 2008,29(2): 371-375 DOI:
ZHOU Li-rong, ZHOU Li-qun, YU Guo-feng, TANG Zi-wei, WANG Fen. Preparation and Properties of SiO<sub>2</sub> Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles[J]. Chinese Journal of Luminescence, 2008,29(2): 371-375 DOI:
以前驱物pAB-DTPAA-APTEOS、正硅酸乙酯(TEOS)和三氯化铕(EuCl3)等为原料
采用油包水(W/O)的反相微乳液法
在正硅酸乙酯(TEOS)和3-氨丙基三乙氧基硅烷(APTEOS)的共同水解下
制备出新型的SiO
2
包覆铕配合物荧光纳米粒子Eu-pAB-DTPAA-AP-SiO
2
。运用TEM、IR、UV-Vis、荧光光谱等技术对荧光纳米粒子进行了表征。TEM结果表明:包覆体呈球形
分散均匀
平均粒径为40nm。纳米粒子与配体、前驱物的紫外吸收谱相比较
峰位发生了一定的红移
表明通过反相微乳液法得到的固体粉末与EuCl3反应后
已经生成配合物Eu-pAB-DTPAA-AP-SiO
2
。红外光谱研究表明
在801cm-1出现ν
Si-C
的伸缩振动峰
471cm-1处出现ν
Eu-O
的伸缩振动峰。由此证实Eu-pAB-DTPAA-AP-SiO
2
配合物的存在。荧光光谱分析表明
纳米粒子Eu-pAB-DTPAA-AP-SiO
2
表现出较好的荧光性能
位于592
615
689nm的发射峰分别归属于Eu
3+
离子的
5
D
0
→
7
F
1
、
5
D
0
→
7
F
2
和
5
D
0
→
7
F
4
跃迁
其中最强峰615nm属于Eu
3+
的特征跃迁发射。作为一种新型的荧光试剂
该纳米粒子具有粒径小
亲水性强
荧光强度大
且表面的氨基能方便地与生物分子偶联
故可作为优良的时间分辨荧光标记物用于各种高灵敏生物检测技术中。
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