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北京交通大学, 光电子技术研究所, 发光与光信息技术教育部重点实验室, 北京, 100044
收稿:2007-01-14,
修回:2007-2-24,
纸质出版:2007-05-20
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封宾, 滕枫, 唐爱伟, 王琰, 侯延冰, 王永生. 氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记[J]. 发光学报, 2007,28(3): 421-424,
FENG Bin, TENG Feng, TANG Ai-wei, WANG Yan, HOU Yan-bing, WANG Yong-sheng. Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme[J]. Chinese Journal of Luminescence, 2007,28(3): 421-424,
封宾, 滕枫, 唐爱伟, 王琰, 侯延冰, 王永生. 氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记[J]. 发光学报, 2007,28(3): 421-424, DOI:
FENG Bin, TENG Feng, TANG Ai-wei, WANG Yan, HOU Yan-bing, WANG Yong-sheng. Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme[J]. Chinese Journal of Luminescence, 2007,28(3): 421-424, DOI:
利用氨基酸中惟一带有巯基的L-半胱氨酸盐酸盐(L-Cys)作为稳定剂在水相体系中合成CdSe纳米晶
通过表面包覆的L-Cys与生物试剂溶菌酶进行结合
实现CdSe纳米晶对溶菌酶的荧光标记.利用TEM、荧光光谱仪及荧光显微镜等多种手段对样品的结构、形貌及光学性能进行表征.试验结果表明
利用L-Cys为稳定剂合成的CdSe纳米晶具有较强的生物活性
L-Cys本身所带的氨基和羧基可以和多种生物试剂通过不同途径进行结合.对溶菌酶(Lys)标记前后
CdSe纳米晶的发射光谱的峰位发生微小红移
大约为2nm
半峰全宽基本保持不变
在40~50nm之间
荧光强度随所标记的Lys浓度的不同均发生明显增强.所有特性均表明实验所合成的CdSe纳米晶在生物标记方面具有很好的实用价值.
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