Aiming at the measurement of the cow bloods

the generation mechanism and characteristic of fluorescent spectra of cow blood were researched in this paper.The fluorescent excitation and spectrum measurement system is fluorescence spectrophotometer RF-5301 (Shimadzu).The experimental sample was the normal blood that was drawn out from the cow vein.When added into some anti-coagulator

the blood samples' spectra were measured.These experimental cows were selected from the several farms in the province of Heilongjiang.They were all healthy cows but different in age.The experiment results show that the fluorescence of the cow bloods can be effectively induced by light wave.The intensity and peak value of the fluorescent spectra are different with the wavelength of the excitation light.In the experiment the wavelength value of fluorescence peaks are found around two places:one peak is near 317 nm and the other is near 367 nm when the wavelengths of the light ranges from 220 to 270 nm.At this time the strife phenomena occur.After we compared the experiment results about the fluorescent spectra with some different excitation light

we find that the fluorescent intensities which are excited by 220

230

240

290

350

480

500 nm light are greater.The fluorescent intensities which are excited by other light are lower and the great peak values appear at around 317

365

367

388

348

463

467

607

638 nm.It is shown that the excitation light would give obvious biologic effect on the cow bloods.This study is valuable for selecting of the best excitation light wave in the technology of fluorescent spectra of cow bloods induced by excitation light.It provides basic information for the early distinguishing and diagnosis of cow diseases in the application of the fluorescence spectrum technology.