ZHUANG Huisheng, CHEN Guonan, HUANG Jinling. STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE[J]. Chinese Journal of Luminescence, 1999,20(4): 358-362
ZHUANG Huisheng, CHEN Guonan, HUANG Jinling. STUDY ON THE CHEMILUMINESCENTPROPERTIES OF ETHIDIUM BROMIDE[J]. Chinese Journal of Luminescence, 1999,20(4): 358-362DOI:
we first investigated the chemiluminescent properties of ethidiumbromide
and find that RNA can enhance the chemiluminescent intensity of reaction between ethidium bromide and KMnO
4
in a strong acid medium. There is a linear relationship between the chemiluminescent intensity obtained in the system of EB-KMnO
4
-RNA and RNA concentration
therefore
a new chemiluminescent analytical method for thedetermination of RNA is established and used to determine RNA in synthetic samples.The linear calibration ranges is 0.10~10.0μg/mL and the detection limit is 0.06μg/mL for RNA
the relative standard deviation is 3.8% for 3.5μg/mL RNA. Interference ionswere examined. The precision
accuracy and selectivity of the method are satisfactory.