One of major factors that have hampered bioluminescent ATP techniques application to biomass assays is the improper removal methods of somatic ATP and inadequate microbial ATP extraction methods that have been used in the ATP rapid microbiology. The experimental study of non-microbial ATP removal methods and the microbial ATP extraction methods has been done. Triton X-100 and apyrase are used as extractant and removal reagent of somatic ATP. Trichlorracetic acid is used to extract microbial ATP. The optimum concentration of them has successively been determined as 0.15%

0.10% and 1.50%. The comparative research of microbial ATP extraction methods such as ultrasonic disintegration

boiling

ultrasonic boiling

TCA and chloroform has been done. The conclusion is that the trichloroacetic acid is preferable to other extraction methods. The method is simple and easy to be widely used.