A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample Enhanced Publication

{ "defaultlang": "zh", "titlegroup": { "articletitle": [ { "lang": "zh", "data": [ { "name": "text", "data": "一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测" } ] }, { "lang": "en", "data": [ { "name": "text", "data": "A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample" } ] } ] }, "contribgroup": { "author": [ { "name": [ { "lang": "zh", "surname": "王", "givenname": "芹", "namestyle": "eastern", "prefix": "" }, { "lang": "en", "surname": "WANG", "givenname": "Qin", "namestyle": "eastern", "prefix": "" } ], "stringName": [], "aff": [ { "rid": "aff1", "text": "" } ], "role": [ "first-author" ], "bio": [ { "lang": "zh", "text": [ "王芹（1985‒），女，安徽阜阳人，博士，副教授，2012年于兰州大学获得博士学位，主要从事纳米荧光探针的设计合成及其性能的研究。" ], "graphic": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879961&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879965&type=", "big": 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"eastern", "prefix": "" }, { "lang": "en", "surname": "HUANG", "givenname": "Pei", "namestyle": "eastern", "prefix": "" } ], "stringName": [], "aff": [ { "rid": "aff1", "text": "" } ], "role": [], "deceased": false }, { "name": [ { "lang": "zh", "surname": "孙", "givenname": "艳丽", "namestyle": "eastern", "prefix": "" }, { "lang": "en", "surname": "SUN", "givenname": "Yan-li", "namestyle": "eastern", "prefix": "" } ], "stringName": [], "aff": [ { "rid": "aff1", "text": "" } ], "role": [], "deceased": false }, { "name": [ { "lang": "zh", "surname": "葛", "givenname": "举", "namestyle": "eastern", "prefix": "" }, { "lang": "en", "surname": "GE", "givenname": "Ju", "namestyle": "eastern", "prefix": "" } ], "stringName": [], "aff": [ { "rid": "aff1", "text": "" } ], "role": [], "deceased": false }, { "name": [ { "lang": "zh", "surname": "张", "givenname": "晟瑞", "namestyle": "eastern", "prefix": "" }, { "lang": "en", "surname": "ZHANG", "givenname": "Sheng-rui", "namestyle": "eastern", "prefix": "" } ], "stringName": [], "aff": [ { "rid": "aff1", "text": "" } ], "role": [ "corresp", "corresp" ], "corresp": [ { "rid": "cor1", "lang": "en", "text": "E-mail： zhangshr09@sina.com", "data": [ { "name": "text", "data": "E-mail： zhangshr09@sina.com" } ] }, { "rid": "cor2", "lang": "en", "text": "zhangshr09@sina.com", "data": [ { "name": "text", "data": "zhangshr09@sina.com" } ] } ], "bio": [ { "lang": "zh", "text": [ "张晟瑞（1985‒），男，甘肃平凉人，博士，教授，2018年于西北大学获得博士学位，主要从事荧光探针的设计合成及其性能的研究。" ], "graphic": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879969&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879975&type=", "big": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879971&type=", "width": "25.00000191", "height": "29.99996185", "fontsize": "" } ], "data": [ [ { "name": "text", "data": "张晟瑞" }, { "name": "text", "data": "（1985‒），男，甘肃平凉人，博士，教授，2018年于西北大学获得博士学位，主要从事荧光探针的设计合成及其性能的研究。" } ] ] } ], "email": "zhangshr09@sina.com", "deceased": false } ], "aff": [ { "id": "aff1", "intro": [ { "lang": "zh", "text": "陕西理工大学 化学与环境科学学院， 陕西省催化基础与应用重点实验室， 陕西 汉中　723000", "data": [ { "name": "text", "data": "陕西理工大学 化学与环境科学学院， 陕西省催化基础与应用重点实验室， 陕西 汉中　723000" } ] }, { "lang": "en", "text": "Shaanxi Key Laboratory of Catalysis， School of Chemistry and Environment Science， Shaanxi University of Technology， Hanzhong 723000， China", "data": [ { "name": "text", "data": "Shaanxi Key Laboratory of Catalysis， School of Chemistry and Environment Science， Shaanxi University of Technology， Hanzhong 723000， China" } ] } ] } ] }, "abstracts": [ { "lang": "zh", "data": [ { "name": "p", "data": [ { "name": "text", "data": "苯硫酚（Thiophenol，PhSH）是一种剧毒物质，对环境和健康危害极大，因此，对环境中PhSH的快速检测具有重要意义。本工作中，碳点基纳米探针（" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "）以2，4-二硝基苯磺酰胺为识别基团，实现了环境样品中PhSH的高选择性检测。探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "溶液本身的荧光强度较弱，加入PhSH后，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "与其发生亲核取代反应，2，4-二硝基苯磺酰胺键断裂，释放出荧光团，导致探针的荧光强度和吸光度显著增强。进一步研究表明，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "在520 nm处的荧光强度和440 nm处的吸光度随着PhSH浓度的增加呈线性增加，线性范围为0~5 μmol/L。该探针对PhSH的检测具有反应速度快（4 min）、选择性好等特点。此外，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "可用于环境水样中PhSH的检测，且可制备成试纸条实现PhSH的半定量可视化检测。实验结果表明，碳点基纳米探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "在环境领域具有广阔的潜在应用前景。" } ] } ] }, { "lang": "en", "data": [ { "name": "p", "data": [ { "name": "text", "data": "Thiophenol（PhSH） is a highly toxic substance， which does great harm to environment and health. Therefore, the rapid detection of PHSH in the environment is of great significance. In this work， the carbon dot（CD）-based nanoprobe " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": " used 2，4-dinitrobenzenesulfonyl as the recognition group was performed to achieve a highly selective detection of PhSH in the environmental samples. The CD-based nanoprobe " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": " solution exhibited very weak fluorescence and absorbance. After adding PhSH， the 2，4-dinitrobenesulfonamide moiety of " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS " } ] }, { "name": "text", "data": "could be readily cleaved " }, { "name": "italic", "data": [ { "name": "text", "data": "via" } ] }, { "name": "text", "data": " nucleophilic substitution reaction， leading to a dramatic increase of fluorescence intensity and absorbance of the probe. Further studies showed that the fluorescence intensity at 520 nm and absorbance at 440 nm of the probe showed a good linear relationship with the PhSH concentration， with a linear range of 0-5 μmol/L. The established detection probe displayed fast reaction（4 min） and high selectivity towards PhSH. In addition， " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": " could be used for the detection of PhSH in environmental water samples， and also could be prepared into a strip to realize the semi-quantitative visual detection of PhSH. The experimental results show that the CD-based nanoprobe " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": " has a potential application prospect in the environmental field." } ] } ] } ], "keyword": [ { "lang": "zh", "data": [ [ { "name": "text", "data": "碳点" } ], [ { "name": "text", "data": "纳米探针" } ], [ { "name": "text", "data": "比色/荧光增强型" } ], [ { "name": "text", "data": "苯硫酚" } ], [ { "name": "text", "data": "环境样品" } ] ] }, { "lang": "en", "data": [ [ { "name": "text", "data": "carbon dots" } ], [ { "name": "text", "data": "nanoprobe" } ], [ { "name": "text", "data": "colorimetric/fluorescent turn-on" } ], [ { "name": "text", "data": "thiophenol" } ], [ { "name": "text", "data": "environmental samples" } ] ] } ], "highlights": [], "body": [ { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "1　引　　言" } ], "level": "1", "id": "s1" } }, { "name": "p", "data": [ { "name": "text", "data": "作为一种重要的工业原料，苯硫酚（Thiophenol, PhSH）可用于化学中间体、染料和农药的制备。然而，长期接触PhSH对人体健康的危害很大，可能会引起中枢神经系统损伤、恶心、肌肉无力、头痛、昏迷甚至死亡" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "1", "type": "bibr", "rid": "R1", "data": [ { "name": "text", "data": "1" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。此外，环境中过量的PhSH会对其他动物和水生生物造成危害。鉴于此，PhSH已被美国环境保护署列为最重要的环境污染物之一。传统检测PhSH的方法，如气相色谱-质谱联用" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "2", "type": "bibr", "rid": "R2", "data": [ { "name": "text", "data": "2" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "和高效液相色谱" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "3", "type": "bibr", "rid": "R3", "data": [ { "name": "text", "data": "3" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "，具有良好的准确性和重现性，但具有成本高、检测时间长等问题，制约了它们在环境样品中快速检测的实际应用。因此，开发一种简单、快速、高选择性检测PhSH的方法在环境科学领域具有重要意义。" } ] }, { "name": "p", "data": [ { "name": "text", "data": "荧光探针具有选择性和灵敏度高、操作方便等优点" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "blockXref", "data": { "data": [ { "name": "xref", "data": { "text": "4", "type": "bibr", "rid": "R4", "data": [ { "name": "text", "data": "4" } ] } }, { "name": "text", "data": "-" }, { "name": "xref", "data": { "text": "5", "type": "bibr", "rid": "R5", "data": [ { "name": "text", "data": "5" } ] } } ], "rid": [ "R4", "R5" ], "text": "4-5", "type": "bibr" } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "，在PhSH的检测中具有广阔的应用前景。然而，由于PhSH和生物硫醇具有相似的物理和化学性质，开发可区分PhSH和生物硫醇的荧光探针极具挑战性" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "6", "type": "bibr", "rid": "R6", "data": [ { "name": "text", "data": "6" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。Wang课题组以2,4 -二硝基苯磺酰胺（2,4-dinitrobenzenesulfonyl, DNS）为识别基团开发了首个可特异性区分PhSH和生物硫醇的荧光探针" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "7", "type": "bibr", "rid": "R7", "data": [ { "name": "text", "data": "7" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。此后，科研工作者以2,4-二硝基苯磺酰胺作为识别基团、半花菁" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "8", "type": "bibr", "rid": "R8", "data": [ { "name": "text", "data": "8" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "或罗丹明" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "9", "type": "bibr", "rid": "R9", "data": [ { "name": "text", "data": "9" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "等染料作为报告基团，开发了许多荧光增强型探针。然而，它们的荧光团大多数为有机小分子，光稳定性较差。因此，寻找较好的荧光团是一项长久且关键的工作。荧光碳点（Carbon dots,CDs）具有合成步骤简单、光稳定性高、毒性低等特点" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "blockXref", "data": { "data": [ { "name": "xref", "data": { "text": "10", "type": "bibr", "rid": "R10", "data": [ { "name": "text", "data": "10" } ] } }, { "name": "text", "data": "-" }, { "name": "xref", "data": { "text": "14", "type": "bibr", "rid": "R14", "data": [ { "name": "text", "data": "14" } ] } } ], "rid": [ "R10", "R11", "R12", "R13", "R14" ], "text": "10-14", "type": "bibr" } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。近年来，很多课题组以CDs为荧光团，开发了很多基于CDs的荧光探针" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "blockXref", "data": { "data": [ { "name": "xref", "data": { "text": "15", "type": "bibr", "rid": "R15", "data": [ { "name": "text", "data": "15" } ] } }, { "name": "text", "data": "-" }, { "name": "xref", "data": { "text": "17", "type": "bibr", "rid": "R17", "data": [ { "name": "text", "data": "17" } ] } } ], "rid": [ "R15", "R16", "R17" ], "text": "15-17", "type": "bibr" } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。然而，可检测环境样品中PhSH的CDs基荧光纳米探针的开发还处于初级阶段。因此，开发一种高选择性、快速响应的CDs基纳米荧光探针用于环境中PhSH的检测是一个亟待解决的问题。" } ] }, { "name": "p", "data": [ { "name": "text", "data": "在前期工作中，我们以黄绿色荧光CDs（Y-G-CDs）（量子产率为28.6%）为荧光团、DNS为识别基团，成功构建了一个检测生物体内硒代半胱氨酸（Selenocysteine,Sec）的CDs基纳米探针（" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "）" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "18", "type": "bibr", "rid": "R18", "data": [ { "name": "text", "data": "18" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。在该工作中，我们并未探讨" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "检测环境中PhSH的应用潜力。考虑到PhSH和Sec具有相似的物理化学性质（PhSH和Sec的p" }, { "name": "italic", "data": [ { "name": "text", "data": "K" } ] }, { "name": "text", "data": "a值分别为6.5和5.2，分子结构中均含有巯基），我们设想将" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "应用于环境中PhSH的检测。结果表明探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "对PhSH表现出明显的比色/荧光增强反应，且反应速度非常快（反应时间为4 min）。" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "溶液本身几乎没有荧光，加入PhSH后，荧光强度增强，且颜色发生显著变化（从无色变成黄色），很容易用肉眼观察到，这意味着" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "可用于PhSH的比色/荧光双模式检测。该纳米荧光探针对PhSH的检测不受其他干扰物质的影响，具有良好的选择性。此外，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "可用于环境水样中PhSH的测定（回收率为94.0% ~ 101.8%），并可制备成试纸条实现PhSH的半定量可视化检测，结果令人满意。" } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2　实　　验" } ], "level": "1", "id": "s2" } }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2.1　材料和仪器" } ], "level": "2", "id": "s2a" } }, { "name": "p", "data": [ { "name": "text", "data": "间氨基酚（" }, { "name": "italic", "data": [ { "name": "text", "data": "m" } ] }, { "name": "text", "data": "-aminophenol," }, { "name": "italic", "data": [ { "name": "text", "data": "m" } ] }, { "name": "text", "data": "AP）、PhSH、2, 4-二硝基苯磺酰氯、L-半胱氨酸（L-cysteine,L-Cys）、高半胱氨酸（Homocysteine,Hcy）、谷胱甘肽（Glutathione,GSH）购自阿拉丁有限公司（上海，中国)，其他所有试剂均为分析纯。" } ] }, { "name": "p", "data": [ { "name": "text", "data": "傅立叶变换红外（Fourier transform infrared, FT-IR）光谱在Vertex 70 FT-IR光谱仪（Bruker，德国）上进行采集。X射线光电子能谱（X-ray photoelectron spectroscopy,XPS）在ESCALAB 250Xi上得到。使用日立FL-4600荧光分光光度计和岛津UV-2600紫外分光光度计分别测量荧光光谱和紫外-可见吸收光谱。溶液pH值通过Sartorius PB-10 pH计测量。" } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2.2　Y-G-CDs制备" } ], "level": "2", "id": "s2b" } }, { "name": "p", "data": [ { "name": "text", "data": "向0.1 g " }, { "name": "italic", "data": [ { "name": "text", "data": "m" } ] }, { "name": "text", "data": "AP的10 mL C" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "H" }, { "name": "sub", "data": [ { "name": "text", "data": "5" } ] }, { "name": "text", "data": "OH溶液中加入15 μL浓盐酸及5 μL浓硝酸，然后将溶液转移到25 mL聚四氟乙烯内衬的不锈钢高压釜中。首先，180 ℃下加热12 h，得到红褐色悬浮液。然后，以CH" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "Cl" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "/ CH" }, { "name": "sub", "data": [ { "name": "text", "data": "3" } ] }, { "name": "text", "data": "OH（5∶1，" }, { "name": "italic", "data": [ { "name": "text", "data": "V" } ] }, { "name": "text", "data": "/" }, { "name": "italic", "data": [ { "name": "text", "data": "V" } ] }, { "name": "text", "data": "）作为洗脱剂，柱色谱纯化粗产物，最终得到纯化的Y-G-CDs样品。" } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2.3　CD-DNS制备" } ], "level": "2", "id": "s2c" } }, { "name": "p", "data": [ { "name": "text", "data": "将20 mg Y-G-CDs加入到含有100 μL三乙胺和2 mL DMF的30 mL CH" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "Cl" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "溶液中，逐滴加入2,4-二硝基苯磺酰氯溶液（200 mg溶于6 mL无水CH" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "Cl" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "中），室温下反应2 h。随后，水洗、无水MgSO" }, { "name": "sub", "data": [ { "name": "text", "data": "4" } ] }, { "name": "text", "data": "干燥、过滤并进行低压蒸馏。粗产物利用柱色谱分离，洗脱剂为CH" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "Cl" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "/CH" }, { "name": "sub", "data": [ { "name": "text", "data": "3" } ] }, { "name": "text", "data": "OH（10∶1，" }, { "name": "italic", "data": [ { "name": "text", "data": "V" } ] }, { "name": "text", "data": "/" }, { "name": "italic", "data": [ { "name": "text", "data": "V" } ] }, { "name": "text", "data": "），旋干后得到深棕色固体" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "。" } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2.4　不同pH值缓冲溶液配制" } ], "level": "2", "id": "s2d" } }, { "name": "p", "data": [ { "name": "text", "data": "pH值在4.9 ~ 9.2范围内的缓冲溶液分别由磷酸氢二钠-柠檬酸缓冲液（pH值范围为2.2 ~ 8.0）和甘氨酸-氢氧化钠缓冲液（pH值范围为8.5 ~ 9.2）制备得到。" } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2.5　探针CD-DNS对分析物的光谱响应" } ], "level": "2", "id": "s2e" } }, { "name": "p", "data": [ { "name": "text", "data": "准确称取10 mg的探针，用丙酮溶解，并定容至10 mL，得到1 mg/mL" }, { "name": "bold", "data": [ { "name": "text", "data": " CD-DNS" } ] }, { "name": "text", "data": "储备液。将" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "溶液（100 μL,1 mg/mL）、约4 mL 磷酸盐缓冲溶液（PBS）和不同体积的PhSH原液（1 mmol/L）加入到10 mL比色管中，最后用PBS稀释至5 mL。充分混合后，记录荧光和紫外-可见吸收光谱。" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "/" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "em" } ] }, { "name": "text", "data": "=450/520 nm，激发和发射光的狭缝宽度均为5 nm。" } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "2.6　CD-DNS法测定环境水样中的PhSH" } ], "level": "2", "id": "s2f" } }, { "name": "p", "data": [ { "name": "text", "data": "采用标准添加法验证" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "对环境水样中PhSH的检测能力。以自来水（本实验室）、湖水（陕西理工大学校园湖水）和河水（汉江汉中段）3个实际环境样品为研究对象，分别采集了3个50 mL的样品。水样在试验前利用孔径为0.22 μm的水相微孔滤膜进行过滤，用PBS缓冲液调节pH值为7.8。将探针和不同浓度（0，1，2，4 μmol/L）的PhSH原液加入等分水样中，室温孵育4 min，记录溶液的荧光强度。" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "/" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "em" } ] }, { "name": "text", "data": "=450/520 nm，激发和发射光的狭缝宽度均为5 nm。" } ] } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "3　结果与讨论" } ], "level": "1", "id": "s3" } }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "3.1　CD-DNS对PhSH响应的可行性" } ], "level": "2", "id": "s3a" } }, { "name": "p", "data": [ { "name": "text", "data": "首先，我们利用加入PhSH前后" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "的荧光和紫外-可见吸收光谱的变化来验证" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "检测PhSH的可行性。如" }, { "name": "xref", "data": { "text": "图1", "type": "fig", "rid": "F2", "data": [ { "name": "text", "data": "图1" } ] } }, { "name": "text", "data": "所示，游离的" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "几乎没有荧光，且440 nm处的紫外吸收强度很弱。加入PhSH后，探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "溶液在520 nm处的荧光强度随着PhSH的加入而增加（365 nm紫外灯照射下，溶液颜色由无色变为黄绿色），而且，探针在440 nm波长下的紫外吸收强度也显著增强（日光灯照射下，溶液颜色由无色变为黄色）。实验结果表明" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "具有比色/荧光双信号检测PhSH的能力。此外，室温下，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "储备液具有很好的稳定性，36 h内其荧光强度不会发生变化（图S1），而且" }, { "name": "bold", "data": [ { "name": "text", "data": "CD⁃DNS" } ] }, { "name": "text", "data": "溶液放置较长时间后，其对PhSH的响应效果和发光颜色没有明显改变（图S2）。" } ] }, { "name": "fig", "data": { "id": "F1", "caption": [ { "lang": "zh", "label": [ { "name": "text", "data": "图1" } ], "title": [ { "name": "text", "data": "探针（20 μg/mL）与PhSH（0，5 μmol/L）在20 mmol/L缓冲液（pH=7.4，25 ℃，" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "=450 nm）中反应后的荧光发射光谱（a）和紫外-可见吸收光谱（b），插图分别为探针（ⅰ）与探针+PhSH（ⅱ）的荧光图像（（a）中插图）和可见光图像（（b）中插图）。" } ] }, { "lang": "en", "label": [ { "name": "text", "data": "Fig. 11" } ], "title": [ { "name": "text", "data": "Fluorescence emission spectra（a） and UV-Vis absorption spectra（b） of the probe（20 μg/mL） reacted with PhSH（0， 5 μmol/L） in 20 mmol/L buffer solution（pH=7.4， 25 ℃， " }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "=450 nm）. Insets are fluorescent images（inset in （a）） and visible light images（inset in （b）） of probe（ⅰ） and probe +PhSH（ⅱ） respectively." } ] } ], "subcaption": [], "note": [], "graphics": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879986&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879986&type=", "big": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879986&type=", "width": "144.58093262", "height": "39.80593872", "fontsize": "" } ] } }, { "name": "p", "data": [ { "name": "text", "data": "接下来，我们分析了探针与PhSH的响应机理。根据前人的报道结果可知当识别基团为DNS时，该探针可选择性区分PhSH和生物硫醇" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "7", "type": "bibr", "rid": "R7", "data": [ { "name": "text", "data": "7" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。因此，本工作拟利用缩合反应，将识别基团2,4-二硝基苯磺酰氯键合在Y-G-CDs表面得到可选择性检测PhSH的探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "。运用XPS和FT-IR对Y-G-CDs的表面组成和化学状态进行表征，说明Y-G-CDs的表面含有—OH和—NH" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "官能团（图S3）" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "18", "type": "bibr", "rid": "R18", "data": [ { "name": "text", "data": "18" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。同时，利用XPS和FT-IR对探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "的元素组成进行表征" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "18", "type": "bibr", "rid": "R18", "data": [ { "name": "text", "data": "18" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。与Y-G-CDs相比，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "包含一个新元素（S2p，164 eV）。在高分辨谱中，N1s的XPS数据分析显示硝基N的含量显著增加。此外，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "的FT-IR光谱表明" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "含有—NO" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "（1 539 cm" }, { "name": "sup", "data": [ { "name": "text", "data": "-1" } ] }, { "name": "text", "data": "和1 345 cm" }, { "name": "sup", "data": [ { "name": "text", "data": "-1" } ] }, { "name": "text", "data": "）和—SO" }, { "name": "sub", "data": [ { "name": "text", "data": "2" } ] }, { "name": "text", "data": "—（1 386 cm" }, { "name": "sup", "data": [ { "name": "text", "data": "-1" } ] }, { "name": "text", "data": "和1 193 cm" }, { "name": "sup", "data": [ { "name": "text", "data": "-1" } ] }, { "name": "text", "data": "）（图S4）。综上所述，DNS被成功连接到了Y-G-CDs表面，得到CDs基纳米探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "。由于Y-G-CDs的表面状态发生变化，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "自身几乎没有荧光，最大吸收波长为355 nm，与Y-G-CDs相比蓝移了85 nm（" }, { "name": "xref", "data": { "text": "图1", "type": "fig", "rid": "F2", "data": [ { "name": "text", "data": "图1" } ] } }, { "name": "text", "data": "）。加入PhSH后，探针在520 nm和440 nm处分别出现新的荧光发射峰和紫外-可见吸收峰，且位置与Y-G-CDs的相一致。实验结果表明，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "可与PhSH发生亲核取代反应，2,4-二硝基苯磺酰胺键断裂，释放出荧光团（" }, { "name": "xref", "data": { "text": "图2", "type": "fig", "rid": "F1", "data": [ { "name": "text", "data": "图2" } ] } }, { "name": "text", "data": "）。因此Y-G-CDs的荧光发射和紫外吸收恢复，最终实现PhSH的高选择性检测。" } ] }, { "name": "fig", "data": { "id": "F2", "caption": [ { "lang": "zh", "label": [ { "name": "text", "data": "图2" } ], "title": [ { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "检测PhSH机理示意图" } ] }, { "lang": "en", "label": [ { "name": "text", "data": "Fig. 2" } ], "title": [ { "name": "text", "data": "Schematic diagram of mechanism for sensing of PhSH by " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] } ] } ], "subcaption": [], "note": [], "graphics": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879990&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879995&type=", "big": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35879993&type=", "width": "144.58093262", "height": "39.80593872", "fontsize": "" } ] } } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "3.2　检测条件优化" } ], "level": "2", "id": "s3b" } }, { "name": "p", "data": [ { "name": "text", "data": "为获得检测PhSH的最佳条件，本文采用单因素试验法考察了pH值对反应的影响。如图 3（a）所示，当添加PhSH时，在4.9 ~ 9.2 的pH范围内，探针溶液在520 nm处的荧光强度先增大后减小，pH=7.8时达到最大值，这是由于强酸条件不利于探针与PhSH间的亲核取代反应，而碱性条件下Y-G-CDs的荧光强度较弱的原因" }, { "name": "sup", "data": [ { "name": "text", "data": "[" }, { "name": "xref", "data": { "text": "18", "type": "bibr", "rid": "R18", "data": [ { "name": "text", "data": "18" } ] } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "。因此，我们选择7.8为最佳反应pH值。此外，我们测定了" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "在加入PhSH后，探针在440 nm处吸光度随时间的变化。如图3（b）所示，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "与PhSH反应迅速，当反应时间为4 min时，反应基本结束，反应速度快，可降低氧化，提高PhSH检测的准确性。因此，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "对PhSH的响应时间控制在4 min。" } ] }, { "name": "fig", "data": { "id": "F3", "caption": [ { "lang": "zh", "label": [ { "name": "text", "data": "图3" } ], "title": [ { "name": "text", "data": "在缓冲溶液（20 mmol/L，λex=450 nm）中，CD-DNS（20 μg/mL）对PhSH（5 μmol/L）的pH依赖性荧光响应（a）和随时间变化的吸收响应（b）。" } ] }, { "lang": "en", "label": [ { "name": "text", "data": "Fig. 3" } ], "title": [ { "name": "text", "data": "pH-dependent fluorescence（a） and time-coursed absorption responses（b） of CD-DNS（20 μg/mL） towards PhSH（5μmol/L）in buffer solution（20 mmol/L，λex=450 nm）" } ] } ], "subcaption": [], "note": [], "graphics": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880004&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880004&type=", "big": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880004&type=", "width": "144.58093262", "height": "39.80593872", "fontsize": "" } ] } } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "3.3　CD-DNS对PhSH的响应" } ], "level": "2", "id": "s3c" } }, { "name": "p", "data": [ { "name": "text", "data": "在最佳条件下，我们测定了探针与不同浓度的PhSH反应后的荧光光谱和紫外-可见吸收光谱。如" }, { "name": "xref", "data": { "text": "图4", "type": "fig", "rid": "F6", "data": [ { "name": "text", "data": "图4" } ] } }, { "name": "text", "data": "（a）所示，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "的荧光强度随PhSH浓度增加而增大，且它们之间呈良好的线性关系，线性范围为0~5 μmol/L，检测限为5.9 nmol/L。线性回归方程为：" }, { "name": "italic", "data": [ { "name": "text", "data": "Y" } ] }, { "name": "text", "data": "=196.95" }, { "name": "italic", "data": [ { "name": "text", "data": "X" } ] }, { "name": "text", "data": "+63.34(" }, { "name": "italic", "data": [ { "name": "text", "data": "r" } ] }, { "name": "text", "data": "=0.997 0)，" }, { "name": "italic", "data": [ { "name": "text", "data": "Y" } ] }, { "name": "text", "data": "为" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "的荧光强度，" }, { "name": "italic", "data": [ { "name": "text", "data": "X" } ] }, { "name": "text", "data": "为PhSH的浓度（" }, { "name": "xref", "data": { "text": "图4", "type": "fig", "rid": "F6", "data": [ { "name": "text", "data": "图4" } ] } }, { "name": "text", "data": "（b））。此外，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "在440 nm处的紫外吸收随浓度的增加而逐渐增大（图S5A），线性回归方程为：" }, { "name": "italic", "data": [ { "name": "text", "data": "Y" } ] }, { "name": "text", "data": "=0.0574" }, { "name": "italic", "data": [ { "name": "text", "data": "X" } ] }, { "name": "text", "data": "+0.0888(" }, { "name": "italic", "data": [ { "name": "text", "data": "r" } ] }, { "name": "text", "data": "=0.9973)，" }, { "name": "italic", "data": [ { "name": "text", "data": "Y" } ] }, { "name": "text", "data": "为" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "的吸光度，" }, { "name": "italic", "data": [ { "name": "text", "data": "X" } ] }, { "name": "text", "data": "为PhSH的浓度（图S5（b）），检测限为105 nmol/L。这些结果表明，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "可实现PhSH含量的比色/荧光双模式检测。" } ] }, { "name": "fig", "data": { "id": "F4", "caption": [ { "lang": "zh", "label": [ { "name": "text", "data": "图4" } ], "title": [ { "name": "text", "data": "（a）在缓冲溶液（20 mmol/L，pH=7.8，" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "=450 nm）中，加入不同浓度（0 ~ 5 μmol/L）PhSH时，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "的荧光发射光谱；（b）" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "在520 nm处的荧光强度与不同浓度PhSH的线性关系。" } ] }, { "lang": "en", "label": [ { "name": "text", "data": "Fig. 4" } ], "title": [ { "name": "text", "data": "（a）Fluorescence emission spectra of " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": " with different concentrations（0-5 μmol/L） of PhSH. （b）The linear relationship between fluorescence intensity of " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": " at 520 nm and PhSH at different concentrations." } ] } ], "subcaption": [], "note": [], "graphics": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880012&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880012&type=", "big": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880012&type=", "width": "144.58093262", "height": "39.80593872", "fontsize": "" } ] } } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "3.4　探针对PhSH的选择性" } ], "level": "2", "id": "s3d" } }, { "name": "p", "data": [ { "name": "text", "data": "在复杂的生物和环境样品中，一种新型荧光探针对分析物的选择性响应非常关键。因此，我们通过观察" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "对不同阳离子（Mg" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Ca" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Cu" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Fe" }, { "name": "sup", "data": [ { "name": "text", "data": "3+" } ] }, { "name": "text", "data": "、Fe" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Al" }, { "name": "sup", "data": [ { "name": "text", "data": "3+" } ] }, { "name": "text", "data": "、Mn" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Pb" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Zn" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Ag" }, { "name": "sup", "data": [ { "name": "text", "data": "+" } ] }, { "name": "text", "data": "、Ba" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "、Cd" }, { "name": "sup", "data": [ { "name": "text", "data": "2+" } ] }, { "name": "text", "data": "，浓度均为500 μmol/L）、阴离子（NO" }, { "name": "sub", "data": [ { "name": "text", "data": "3" } ] }, { "name": "sup", "data": [ { "name": "text", "data": "-" } ] }, { "name": "text", "data": "、SO" }, { "name": "sub", "data": [ { "name": "text", "data": "4" } ] }, { "name": "sup", "data": [ { "name": "text", "data": "2-" } ] }, { "name": "text", "data": "、CO" }, { "name": "sub", "data": [ { "name": "text", "data": "3" } ] }, { "name": "sup", "data": [ { "name": "text", "data": "2‒" } ] }, { "name": "text", "data": "、SO" }, { "name": "sub", "data": [ { "name": "text", "data": "3" } ] }, { "name": "sup", "data": [ { "name": "text", "data": "2‒" } ] }, { "name": "text", "data": "、F" }, { "name": "sup", "data": [ { "name": "text", "data": "‒" } ] }, { "name": "text", "data": "、Cl" }, { "name": "sup", "data": [ { "name": "text", "data": "-" } ] }, { "name": "text", "data": "、Br" }, { "name": "sup", "data": [ { "name": "text", "data": "‒" } ] }, { "name": "text", "data": "、SCN" }, { "name": "sup", "data": [ { "name": "text", "data": "‒" } ] }, { "name": "text", "data": "，浓度均为500 μmol/L）、氨基酸（Ala、Trp、Leu、Phe、Thr，浓度均为500 μmol/L）、生物硫醇（Cys、Hcy、GSH，浓度均为500 μmol/L）和PhSH（5 μmol/L）的荧光强度和吸光度响应来评价其对PhSH的选择性。如" }, { "name": "xref", "data": { "text": "图5", "type": "fig", "rid": "F7", "data": [ { "name": "text", "data": "图5" } ] } }, { "name": "text", "data": "所示，除Cys、Hcy和GSH引起探针较弱的比色/荧光强度增强外，其他分析物没有引起探针任何比色/荧光信号的变化。相比之下，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "在PhSH存在下表现出明显的比色/荧光增强。结果表明，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "可用于样品中PhSH的高选择性检测。" } ] }, { "name": "fig", "data": { "id": "F5", "caption": [ { "lang": "zh", "label": [ { "name": "text", "data": "图5" } ], "title": [ { "name": "text", "data": "在缓冲溶液（20 mmol/L，pH=7.8，" }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "=450 nm）中，" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "（20 μg/mL）在不同离子、氨基酸、PhSH存在下的荧光强度（a）和吸光度（b）。" } ] }, { "lang": "en", "label": [ { "name": "text", "data": "Fig.5" } ], "title": [ { "name": "text", "data": "Fluorescence intensity（a） and absorbance（b） of " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "（20 μg/mL） in the presence of different ions， amino acids and PhSH in buffer solution（20 mmol/L，pH=7.8， " }, { "name": "italic", "data": [ { "name": "text", "data": "λ" } ] }, { "name": "sub", "data": [ { "name": "text", "data": "ex" } ] }, { "name": "text", "data": "=450 nm）." } ] } ], "subcaption": [], "note": [], "graphics": [ { "print": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880018&type=", "small": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880018&type=", "big": "http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=35880018&type=", "width": "144.58093262", "height": "39.80593872", "fontsize": "" } ] } } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "3.5　环境水样中PhSH的检测" } ], "level": "2", "id": "s3e" } }, { "name": "p", "data": [ { "name": "text", "data": "由于PhSH对环境具有很高的毒性，因此对环境水样中的PhSH进行监测是必不可少的。本文采用标准添加法验证" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "测定环境自来水、湖水和汉江水样中PhSH浓度的能力，证实其在环境科学中的实用价值。所有的水样都先过滤，调节pH值至7.8。向这些水样中添加不同浓度的PhSH（0，1，2，4 μmol/L），然后加入" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "，反应4 min后进行检测。如" }, { "name": "xref", "data": { "text": "表1", "type": "table", "rid": "T1", "data": [ { "name": "text", "data": "表1" } ] } }, { "name": "text", "data": "所示，回收率在94.0% ~ 101.8%之间。为了进一步考察这个方法的实用性，我们运用试纸条来检测水样中的PhSH。首先，将滤纸浸泡在含有1 mg/mL " }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "的丙酮溶液中，在空气中干燥之后，形成试纸条。然后，在室温下，将该试纸条放入不同浓度的PhSH溶液中，反应4 min后，取出在空气中干燥，观察颜色变化。如" }, { "name": "xref", "data": { "text": "图6", "type": "fig", "rid": "F8", "data": [ { "name": "text", "data": "图6" } ] } }, { "name": "text", "data": "所示，随着PhSH溶液浓度的增加，在日光灯下，试纸条的颜色从浅黄色逐渐变化到深黄色；而在365 nm紫外光照射下，试纸条颜色由无色逐渐变成亮黄绿色。以上结果表明" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "拥有检测实际环境样品中PhSH的能力。" } ] }, { "name": "table", "data": { "id": "T1", "caption": [ { "lang": "zh", "label": [ { "name": "text", "data": "表1" } ], "title": [ { "name": "text", "data": "CD-DNS检测环境水样中的PhSH（" }, { "name": "italic", "data": [ { "name": "text", "data": "n" } ] }, { "name": "text", "data": "=3）" } ] }, { "lang": "en", "label": [ { "name": "text", "data": "Tab.1" } ], "title": [ { "name": "text", "data": "Detection of PhSH in environmental water samples by " }, { "name": "bold", "data": [ { "name": "text", "data": "CD" } ] }, { "name": "text", "data": "-" }, { "name": "bold", "data": [ { "name": "text", "data": "DNS" } ] }, { "name": "text", "data": "（" }, { "name": "italic", "data": [ { "name": "text", "data": "n" } ] }, { "name": "text", "data": "=3）" } ] } ], "note": [ { "lang": "zh", "data": [ { "name": "p", "data": [ { "name": "sup", "data": [ { "name": "text", "data": "a" } ] }, { "name": "text", "data": "三次测量平均值；" }, { "name": "sup", "data": [ { "name": "text", "data": "b" } ] }, { "name": "text", "data": "标准偏差。" } ] } ] } ], "table": [ { "head": [ [ { "align": "center", "style": "border-top:solid;border-bottom:solid;", "data": [ { "name": "text", "data": "样品" } ] }, { "align": "center", "style": "border-top:solid;border-bottom:solid;", "data": [ { "name": "p", "data": [ { "name": 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"R21", "R22" ], "text": "19-22", "type": "bibr" } }, { "name": "text", "data": "]" } ] }, { "name": "text", "data": "，但该方法的线性范围还需在后续的工作中继续改进。" } ] } ] } ] }, { "name": "sec", "data": [ { "name": "sectitle", "data": { "title": [ { "name": "text", "data": "4　结　　论" } ], "level": "1", "id": "s4" } }, { "name": "p", "data": [ { "name": "text", "data": "本工作成功地将CDs基纳米探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "应用于环境样品中PhSH的比色/荧光双模式检测。加入PhSH后，该探针表现出显著的显色反应和荧光“开启”响应，具有反应速度快（4 min）、选择性好的特点。此外，探针" }, { "name": "bold", "data": [ { "name": "text", "data": "CD-DNS" } ] }, { "name": "text", "data": "可成功用于监测环境水中PhSH的含量，回收率较好（94.0% ~ 101.8%），且可制备成试纸条实现PhSH的半定量可视化检测。该实验方法为设计构建新型CDs基纳米探针提供了新思路，拓宽了纳米探针在环境样品中的应用前景。" } ] }, { "name": "p", "data": [ { "name": "text", "data": "本文补充文件及专家审稿意见和作者回复信下 载地址：" }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "http://cjl.lightpublishing.cn/thesisDetails#10. 37188/ CJL.20220044." } ], "href": "http://cjl.lightpublishing.cn/thesisDetails#10.37188/CJL.20220044." } } ] } ] } ], "footnote": [], "reflist": { "title": [ { "name": "text", "data": "参考文献" } ], "data": [ { "id": "R1", "label": "1", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "SUN Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "YANG S H" }, { "name": "text", "data": "，" }, { "name": "text", "data": "WU L" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A highly sensitive and selective fluorescent probe for thiophenol designed " }, { "name": "italic", "data": [ { "name": "text", "data": "via" } ] }, { "name": "text", "data": " a twist-blockage strategy" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Anal. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2016" }, { "name": "text", "data": "，" }, { "name": "text", "data": "88" }, { "name": "text", "data": "（" }, { "name": "text", "data": "4" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "2266" }, { "name": "text", "data": "-" }, { "name": "text", "data": "2272" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1021/acs.analchem.5b04029" } ], "href": "http://dx.doi.org/10.1021/acs.analchem.5b04029" } } ], "title": "A highly sensitive and selective fluorescent probe for thiophenol designed via a twist-blockage strategy" } ] }, { "id": "R2", "label": "2", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "WANG T W" }, { "name": "text", "data": "，" }, { "name": "text", "data": "CHAMBERLAIN E" }, { "name": "text", "data": "，" }, { "name": "text", "data": "SHI H L" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Identification of hydrolytic metabolites of dyfonate in alkaline aqueous solutions by using high performance liquid chromatography-UV detection and gas chromatography-mass spectrometry" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Int. J. Environ. Anal. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2010" }, { "name": "text", "data": "，" }, { "name": "text", "data": "90" }, { "name": "text", "data": "（" }, { "name": "text", "data": "12" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "948" }, { "name": "text", "data": "-" }, { "name": "text", "data": "961" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1080/03067310902874889" } ], "href": "http://dx.doi.org/10.1080/03067310902874889" } } ], "title": "Identification of hydrolytic metabolites of dyfonate in alkaline aqueous solutions by using high performance liquid chromatography-UV detection and gas chromatography-mass spectrometry" } ] }, { "id": "R3", "label": "3", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "HE J X" }, { "name": "text", "data": "，" }, { "name": "text", "data": "AKAO T" }, { "name": "text", "data": "，" }, { "name": "text", "data": "TANI T" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Development of a simple HPLC method for determination of paeoniflorin-metabolizing activity of intestinal bacteria in rat feces" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chem. Pharm. Bull." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2002" }, { "name": "text", "data": "，" }, { "name": "text", "data": "50" }, { "name": "text", "data": "（" }, { "name": "text", "data": "9" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1233" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1237" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1248/cpb.50.1233" } ], "href": "http://dx.doi.org/10.1248/cpb.50.1233" } } ], "title": "Development of a simple HPLC method for determination of paeoniflorin-metabolizing activity of intestinal bacteria in rat feces" } ] }, { "id": "R4", "label": "4", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "刘红文" }, { "name": "text", "data": "，" }, { "name": "text", "data": "朱隆民" }, { "name": "text", "data": "，" }, { "name": "text", "data": "娄霄峰" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "用于细胞和组织中弗林蛋白酶特异性成像的双光子荧光探针研究" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "化学学报" }, { "name": "text", "data": "，" }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "78" }, { "name": "text", "data": "（" }, { "name": "text", "data": "11" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1240" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1245" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.6023/A20070323" } ], "href": "http://dx.doi.org/10.6023/A20070323" } } ], "title": "用于细胞和组织中弗林蛋白酶特异性成像的双光子荧光探针研究" }, { "lang": "en", "text": [ { "name": "text", "data": "LIU H W" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHU L M" }, { "name": "text", "data": "，" }, { "name": "text", "data": "LOU X F" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A two-photon fluorescent probe for specific imaging of furin activity in living cells and tissues" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Acta Chim. Sinica" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "78" }, { "name": "text", "data": "（" }, { "name": "text", "data": "11" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1240" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1245" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.6023/A20070323" } ], "href": "http://dx.doi.org/10.6023/A20070323" } } ], "title": "A two-photon fluorescent probe for specific imaging of furin activity in living cells and tissues" } ] }, { "id": "R5", "label": "5", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "罗凡" }, { "name": "text", "data": "，" }, { "name": "text", "data": "郑东滨" }, { "name": "text", "data": "，" }, { "name": "text", "data": "阳志强" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "新型姜黄素衍生物荧光探针的合成及对半胱氨酸的选择性识别" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "发光学报" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "11" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1810" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1817" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20210211" } ], "href": "http://dx.doi.org/10.37188/CJL.20210211" } } ], "title": "新型姜黄素衍生物荧光探针的合成及对半胱氨酸的选择性识别" }, { "lang": "en", "text": [ { "name": "text", "data": "LUO F" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHENG D B" }, { "name": "text", "data": "，" }, { "name": "text", "data": "YANG Z Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A novel curcumin derivatives fluorescent probe for selective recognition of cysteine" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Lumin." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "11" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1810" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1817" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20210211" } ], "href": "http://dx.doi.org/10.37188/CJL.20210211" } } ], "title": "A novel curcumin derivatives fluorescent probe for selective recognition of cysteine" } ] }, { "id": "R6", "label": "6", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "SHAO X M" }, { "name": "text", "data": "，" }, { "name": "text", "data": "KANG R X" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHANG Y L" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Highly selective and sensitive 1-amino BODIPY-based red fluorescent probe for thiophenols with high off-to-on contrast ratio" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Anal. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2015" }, { "name": "text", "data": "，" }, { "name": "text", "data": "87" }, { "name": "text", "data": "（" }, { "name": "text", "data": "1" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "399" }, { "name": "text", "data": "-" }, { "name": "text", "data": "405" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1021/ac5028947" } ], "href": "http://dx.doi.org/10.1021/ac5028947" } } ], "title": "Highly selective and sensitive 1-amino BODIPY-based red fluorescent probe for thiophenols with high off-to-on contrast ratio" } ] }, { "id": "R7", "label": "7", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "JIANG W" }, { "name": "text", "data": "，" }, { "name": "text", "data": "FU Q Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "FAN H Y" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A highly selective fluorescent probe for thiophenols" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Angew. Chem. Int. Ed." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2007" }, { "name": "text", "data": "，" }, { "name": "text", "data": "46" }, { "name": "text", "data": "（" }, { "name": "text", "data": "44" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "8445" }, { "name": "text", "data": "-" }, { "name": "text", "data": "8448" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1002/anie.200702271" } ], "href": "http://dx.doi.org/10.1002/anie.200702271" } } ], "title": "A highly selective fluorescent probe for thiophenols" } ] }, { "id": "R8", "label": "8", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "ZHANG S R" }, { "name": "text", "data": "，" }, { "name": "text", "data": "WANG Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "WU F F" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Merocyanine-based turn-on fluorescent probe for the sensitive and selective determination of thiophenols " }, { "name": "italic", "data": [ { "name": "text", "data": "via" } ] }, { "name": "text", "data": " a p" }, { "name": "italic", "data": [ { "name": "text", "data": "K" } ] }, { "name": "text", "data": "a shift mechanism" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Talanta" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "216" }, { "name": "text", "data": "：" }, { "name": "text", "data": "120965-1" }, { "name": "text", "data": "-" }, { "name": "text", "data": "7" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1016/j.talanta.2020.120965" } ], "href": "http://dx.doi.org/10.1016/j.talanta.2020.120965" } } ], "title": "Merocyanine-based turn-on fluorescent probe for the sensitive and selective determination of thiophenols via a pKa shift mechanism" } ] }, { "id": "R9", "label": "9", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "WU J J" }, { "name": "text", "data": "，" }, { "name": "text", "data": "YE Z" }, { "name": "text", "data": "，" }, { "name": "text", "data": "WU F" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A rhodamine-based fluorescent probe for colorimetric and fluorescence lighting-up determination of toxic thiophenols in environmental water and living cells" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Talanta" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2018" }, { "name": "text", "data": "，" }, { "name": "text", "data": "181" }, { "name": "text", "data": "：" }, { "name": "text", "data": "239" }, { "name": "text", "data": "-" }, { "name": "text", "data": "247" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1016/j.talanta.2018.01.028" } ], "href": "http://dx.doi.org/10.1016/j.talanta.2018.01.028" } } ], "title": "A rhodamine-based fluorescent probe for colorimetric and fluorescence lighting-up determination of toxic thiophenols in environmental water and living cells" } ] }, { "id": "R10", "label": "10", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "PERUMAL S" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ATCHUDAN R" }, { "name": "text", "data": "，" }, { "name": "text", "data": "EDISON T N J I" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Sustainable synthesis of multifunctional carbon dots using biomass and their applications：a mini-review" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "J. Environ. Chem. Eng." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "9" }, { "name": "text", "data": "（" }, { "name": "text", "data": "4" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "105802" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1016/j.jece.2021.105802" } ], "href": "http://dx.doi.org/10.1016/j.jece.2021.105802" } } ], "title": "Sustainable synthesis of multifunctional carbon dots using biomass and their applications：a mini-review" } ] }, { "id": "R11", "label": "11", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "WAREING T C" }, { "name": "text", "data": "，" }, { "name": "text", "data": "GENTILE P" }, { "name": "text", "data": "，" }, { "name": "text", "data": "PHAN A N" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Biomass-based carbon dots：current development and future perspectives" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "ACS Nano" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "15" }, { "name": "text", "data": "（" }, { "name": "text", "data": "10" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "15471" }, { "name": "text", "data": "-" }, { "name": "text", "data": "15501" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1021/acsnano.1c03886" } ], "href": "http://dx.doi.org/10.1021/acsnano.1c03886" } } ], "title": "Biomass-based carbon dots：current development and future perspectives" } ] }, { "id": "R12", "label": "12", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "陈丽娟" }, { "name": "text", "data": "，" }, { "name": "text", "data": "刘仁勇" }, { "name": "text", "data": "，" }, { "name": "text", "data": "赵丹" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "聚乙烯亚胺修饰的碳点荧光探针用于检测汞离子" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "分析化学" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "48" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1067" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1074" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.19756/j.issn.0253-3820.191528" } ], "href": "http://dx.doi.org/10.19756/j.issn.0253-3820.191528" } } ], "title": "聚乙烯亚胺修饰的碳点荧光探针用于检测汞离子" }, { "lang": "en", "text": [ { "name": "text", "data": "CHEN L J" }, { "name": "text", "data": "，" }, { "name": "text", "data": "LIU R Y" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHAO D" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A novel fluorescent probe for mercury ion detection based on polyethyleneimine modified carbon dots" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Anal. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "48" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1067" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1074" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.19756/j.issn.0253-3820.191528" } ], "href": "http://dx.doi.org/10.19756/j.issn.0253-3820.191528" } } ], "title": "A novel fluorescent probe for mercury ion detection based on polyethyleneimine modified carbon dots" } ] }, { "id": "R13", "label": "13", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "刘俊" }, { "name": "text", "data": "，" }, { "name": "text", "data": "张熙荣" }, { "name": "text", "data": "，" }, { "name": "text", "data": "熊焕明" }, { "name": "text", "data": ". " }, { "name": "text", "data": "荧光碳点在指纹检测中的应用" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "发光学报" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1095" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1113" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20200334" } ], "href": "http://dx.doi.org/10.37188/CJL.20200334" } } ], "title": "荧光碳点在指纹检测中的应用" }, { "lang": "en", "text": [ { "name": "text", "data": "LIU J" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHANG X R" }, { "name": "text", "data": "，" }, { "name": "text", "data": "XIONG H M" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Application of fluorescent carbon dots in fingerprint detection" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Lumin." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1095" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1113" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20200334" } ], "href": "http://dx.doi.org/10.37188/CJL.20200334" } } ], "title": "Application of fluorescent carbon dots in fingerprint detection" } ] }, { "id": "R14", "label": "14", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "宋金萍" }, { "name": "text", "data": "，" }, { "name": "text", "data": "马琦" }, { "name": "text", "data": "，" }, { "name": "text", "data": "梁晓敏" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "高量子产率P，N掺杂碳点用于苦味酸检测及荧光上染研究" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "分析试验室" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "40" }, { "name": "text", "data": "（" }, { "name": "text", "data": "11" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1265" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1268" }, { "name": "text", "data": "." } ], "title": "高量子产率P，N掺杂碳点用于苦味酸检测及荧光上染研究" }, { "lang": "en", "text": [ { "name": "text", "data": "SONG J P" }, { "name": "text", "data": "，" }, { "name": "text", "data": "MA Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "LIANG X M" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "P，N-doped carbon dots with high quantum yield for the detection of picric acid and fluorescent dying study" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Anal. Lab." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "40" }, { "name": "text", "data": "（" }, { "name": "text", "data": "11" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1265" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1268" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" } ], "title": "P，N-doped carbon dots with high quantum yield for the detection of picric acid and fluorescent dying study" } ] }, { "id": "R15", "label": "15", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "李庆芝" }, { "name": "text", "data": "，" }, { "name": "text", "data": "周奕华" }, { "name": "text", "data": "，" }, { "name": "text", "data": "陈袁" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "比率型碳点荧光传感器检测机理与应用研究进展" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "发光学报" }, { "name": "text", "data": "，" }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "41" }, { "name": "text", "data": "（" }, { "name": "text", "data": "5" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "579" }, { "name": "text", "data": "-" }, { "name": "text", "data": "591" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.3788/fgxb20204105.0579" } ], "href": "http://dx.doi.org/10.3788/fgxb20204105.0579" } } ], "title": "比率型碳点荧光传感器检测机理与应用研究进展" }, { "lang": "en", "text": [ { "name": "text", "data": "LI Q Z" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHOU Y H" }, { "name": "text", "data": "，" }, { "name": "text", "data": "CHEN Y" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Research progress on detection mechanism and application of carbon dots-based ratiometric fluorescence sensor" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Lumin." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "41" }, { "name": "text", "data": "（" }, { "name": "text", "data": "5" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "579" }, { "name": "text", "data": "-" }, { "name": "text", "data": "591" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.3788/fgxb20204105.0579" } ], "href": "http://dx.doi.org/10.3788/fgxb20204105.0579" } } ], "title": "Research progress on detection mechanism and application of carbon dots-based ratiometric fluorescence sensor" } ] }, { "id": "R16", "label": "16", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "张震" }, { "name": "text", "data": "，" }, { "name": "text", "data": "曲丹" }, { "name": "text", "data": "，" }, { "name": "text", "data": "安丽" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "荧光碳点的制备、发光机理及应用" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "发光学报" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1125" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1140" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20210061" } ], "href": "http://dx.doi.org/10.37188/CJL.20210061" } } ], "title": "荧光碳点的制备、发光机理及应用" }, { "lang": "en", "text": [ { "name": "text", "data": "ZHANG Z" }, { "name": "text", "data": "，" }, { "name": "text", "data": "QU D" }, { "name": "text", "data": "，" }, { "name": "text", "data": "AN L" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Preparation，luminescence mechanism and application of fluorescent carbon dots" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Lumin." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1125" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1140" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20210061" } ], "href": "http://dx.doi.org/10.37188/CJL.20210061" } } ], "title": "Preparation，luminescence mechanism and application of fluorescent carbon dots" } ] }, { "id": "R17", "label": "17", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "南福春" }, { "name": "text", "data": "，" }, { "name": "text", "data": "薛小矿" }, { "name": "text", "data": "，" }, { "name": "text", "data": "葛介超" }, { "name": "text", "data": "，" }, { "name": "text", "data": "等" }, { "name": "text", "data": ". " }, { "name": "text", "data": "红光/近红外光响应碳点在肿瘤治疗中的应用进展" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "发光学报" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1155" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1171" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20210163" } ], "href": "http://dx.doi.org/10.37188/CJL.20210163" } } ], "title": "红光/近红外光响应碳点在肿瘤治疗中的应用进展" }, { "lang": "en", "text": [ { "name": "text", "data": "NAN F C" }, { "name": "text", "data": "，" }, { "name": "text", "data": "XUE X K" }, { "name": "text", "data": "，" }, { "name": "text", "data": "GE J C" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Recent advances of red/near infrared light responsive carbon dots for tumor therapy" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Lumin." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "42" }, { "name": "text", "data": "（" }, { "name": "text", "data": "8" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1155" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1171" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.37188/CJL.20210163" } ], "href": "http://dx.doi.org/10.37188/CJL.20210163" } } ], "title": "Recent advances of red/near infrared light responsive carbon dots for tumor therapy" } ] }, { "id": "R18", "label": "18", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "WANG Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHANG S R" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHONG Y G" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Preparation of yellow-green-emissive carbon dots and their application in constructing a fluorescent turn-on nanoprobe for imaging of selenol in living cells" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Anal. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2017" }, { "name": "text", "data": "，" }, { "name": "text", "data": "89" }, { "name": "text", "data": "（" }, { "name": "text", "data": "3" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1734" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1741" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1021/acs.analchem.6b03983" } ], "href": "http://dx.doi.org/10.1021/acs.analchem.6b03983" } } ], "title": "Preparation of yellow-green-emissive carbon dots and their application in constructing a fluorescent turn-on nanoprobe for imaging of selenol in living cells" } ] }, { "id": "R19", "label": "19", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "WU Y Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "SHI A P" }, { "name": "text", "data": "，" }, { "name": "text", "data": "LIU H Y" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol " }, { "name": "italic", "data": [ { "name": "text", "data": "in" } ] }, { "name": "text", "data": " " }, { "name": "italic", "data": [ { "name": "text", "data": "vitro" } ] }, { "name": "text", "data": " and " }, { "name": "italic", "data": [ { "name": "text", "data": "in" } ] }, { "name": "text", "data": " " }, { "name": "italic", "data": [ { "name": "text", "data": "vivo" } ] }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "New J. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "44" }, { "name": "text", "data": "（" }, { "name": "text", "data": "40" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "17360" }, { "name": "text", "data": "-" }, { "name": "text", "data": "17367" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1039/d0nj03370g" } ], "href": "http://dx.doi.org/10.1039/d0nj03370g" } } ], "title": "A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol in vitro and in vivo" } ] }, { "id": "R20", "label": "20", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "WU Q Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "WANG J B" }, { "name": "text", "data": "，" }, { "name": "text", "data": "LIANG W L" }, { "name": "text", "data": ". " }, { "name": "text", "data": "A red-to-near-infrared fluorescent probe for the detection of thiophenol based on a novel hydroxylflavone-quinoline-amino molecular system with large Stokes shift" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Dyes Pigm." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "190" }, { "name": "text", "data": "：" }, { "name": "text", "data": "109289-1" }, { "name": "text", "data": "-" }, { "name": "text", "data": "8" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1016/j.dyepig.2021.109289" } ], "href": "http://dx.doi.org/10.1016/j.dyepig.2021.109289" } } ], "title": "A red-to-near-infrared fluorescent probe for the detection of thiophenol based on a novel hydroxylflavone-quinoline-amino molecular system with large Stokes shift" } ] }, { "id": "R21", "label": "21", "citation": [ { "lang": "en", "text": [ { "name": "text", "data": "XU T" }, { "name": "text", "data": "，" }, { "name": "text", "data": "ZHAO S J" }, { "name": "text", "data": "，" }, { "name": "text", "data": "WU X L" }, { "name": "text", "data": "，" }, { "name": "text", "data": "et al" }, { "name": "text", "data": ". " }, { "name": "text", "data": "β-cyclodextrin-promoted colorimetric and fluorescence turn-on probe for discriminating highly toxic thiophenol from biothiols" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "ACS Sustainable Chem. Eng." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2020" }, { "name": "text", "data": "，" }, { "name": "text", "data": "8" }, { "name": "text", "data": "（" }, { "name": "text", "data": "16" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "6413" }, { "name": "text", "data": "-" }, { "name": "text", "data": "6421" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.1021/acssuschemeng.0c00766" } ], "href": "http://dx.doi.org/10.1021/acssuschemeng.0c00766" } } ], "title": "β-cyclodextrin-promoted colorimetric and fluorescence turn-on probe for discriminating highly toxic thiophenol from biothiols" } ] }, { "id": "R22", "label": "22", "citation": [ { "lang": "zh", "text": [ { "name": "text", "data": "张改清" }, { "name": "text", "data": "，" }, { "name": "text", "data": "阴彩霞" }, { "name": "text", "data": ". " }, { "name": "text", "data": "基于二氰基异佛尔酮的荧光探针在检测苯硫酚中的应用" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "无机化学学报" }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "37" }, { "name": "text", "data": "（" }, { "name": "text", "data": "7" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1245" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1250" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.11862/CJIC.2021.161" } ], "href": "http://dx.doi.org/10.11862/CJIC.2021.161" } } ], "title": "基于二氰基异佛尔酮的荧光探针在检测苯硫酚中的应用" }, { "lang": "en", "text": [ { "name": "text", "data": "ZHANG G Q" }, { "name": "text", "data": "，" }, { "name": "text", "data": "YIN C X" }, { "name": "text", "data": ". " }, { "name": "text", "data": "Application of fluorescent probe based on dicyanoisophorone in detection of thiophenol" }, { "name": "text", "data": " ［J］. " }, { "name": "text", "data": "Chin. J. Inorg. Chem." }, { "name": "text", "data": "， " }, { "name": "text", "data": "2021" }, { "name": "text", "data": "，" }, { "name": "text", "data": "37" }, { "name": "text", "data": "（" }, { "name": "text", "data": "7" }, { "name": "text", "data": "）：" }, { "name": "text", "data": "1245" }, { "name": "text", "data": "-" }, { "name": "text", "data": "1250" }, { "name": "text", "data": ". " }, { "name": "text", "data": "（in Chinese）" }, { "name": "text", "data": ". " }, { "name": "text", "data": " doi: " }, { "name": "extlink", "data": { "text": [ { "name": "text", "data": "10.11862/CJIC.2021.161" } ], "href": "http://dx.doi.org/10.11862/CJIC.2021.161" } } ], "title": "Application of fluorescent probe based on dicyanoisophorone in detection of thiophenol" } ] } ] }, "response": [], "contributions": [], "acknowledgements": [], "conflict": [], "supportedby": [], "articlemeta": { "doi": "10.37188/CJL.20220044", "clc": [ [ { "name": "text", "data": "O657.3" } ] ], "dc": [ { "name": "text", "data": "A" } ], "publisherid": "1000-7032（2022）06-0986-09", "citeme": [ { "data": [ { "name": "text", "data": "王芹,刘智峰,李利华等.一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测[J].发光学报,2022,43(06):986-994." } ], "text": "王芹,刘智峰,李利华等.一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测[J].发光学报,2022,43(06):986-994." }, { "data": [ { "name": "text", "data": "WANG Qin,LIU Zhi-feng,LI Li-hua,et al.A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample[J].Chinese Journal of Luminescence,2022,43(06):986-994." } ], "text": "WANG Qin,LIU Zhi-feng,LI Li-hua,et al.A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample[J].Chinese Journal of Luminescence,2022,43(06):986-994." } ], "fundinggroup": [ { "lang": "zh", "text": [ { "name": "text", "data": "国家自然科学基金（22177066）； 陕西省创新能力支撑计划（2021KJXX-51）； 陕西省教育厅科学研究计划（20JK0575）； 陕西省自然科学基础研究计划（2021JQ-751）； 秦巴生物资源与生态环境国家重点实验室（培育）“市校共建”科研专项（SXJ-2103，SXJ-2105）资助项目" } ] }, { "lang": "en", "text": [ { "name": "text", "data": "Support by National Natural Science Foundation of China(22177066)；Innovation Capability Support Program of Shaanxi(2021KJXX-51)；Scientific Research Program Funded by Education Department of Shaanxi Provincial Government(20JK0575)；Natural Science Basic Research Program of Shaanxi(2021JQ-751)；Co-construction Project of Hanzhong City and Shaanxi University of Technology(SXJ-2103，SXJ-2105)" } ] } ], "history": { "received": "2022-02-10", "revised": "2022-03-08", "ppub": "2022-06-05", "opub": "2022-10-19" } }, "appendix": [], "type": "research-article", "ethics": [], "supplementary": [], "journalTitle": "发光学报", "issue": "6", "volume": "43", "originalSource": [] }