一种线粒体靶向香豆素基pH荧光探针合成及性能

陈俊; 冯燕; 孟祥明

doi:10.37188/CJL.20200363

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一种线粒体靶向香豆素基pH荧光探针合成及性能

材料合成及性能 | 更新时间：2021-04-23

- 一种线粒体靶向香豆素基pH荧光探针合成及性能
- Synthesis and Properties of A Mitochondria-targeted Coumarin-based pH Fluorescent Probe
- 发光学报 2021年42卷第4期页码：462-469
- 作者机构：
  
  安徽大学化学化工学院，安徽　合肥　 230601
- 作者简介：
  
  [ "陈俊(1994-)，男，河南信阳人，硕士研究生，2018年于河南科技学院获得学士学位，主要从事荧光探针的合成及性能的研究。E-mail: 649901225@qq.com" ]
  [ "孟祥明(1980-)，男，安徽六安人，博士，教授，博士研究生导师，2007年于中国科学技术大学获得博士学位，主要从事荧光化学传感器的设计合成及新型医药农药的合成工艺开发的研究。E-mail: mengxm@ahu.edu.cn" ]
- 基金信息：
  
  国家自然科学基金(21778001;21672001)
- DOI：10.37188/CJL.20200363
  中图分类号： O482.31
- 纸质出版日期：2021-04-01，
  
  收稿日期：2020-11-29，
  
  修回日期：2020-12-31，
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陈俊, 冯燕, 孟祥明. 一种线粒体靶向香豆素基pH荧光探针合成及性能[J]. 发光学报, 2021,42(4):462-469.

Jun CHEN, Yan FENG, Xiang-ming MENG. Synthesis and Properties of A Mitochondria-targeted Coumarin-based pH Fluorescent Probe[J]. Chinese Journal of Luminescence, 2021,42(4):462-469.
陈俊, 冯燕, 孟祥明. 一种线粒体靶向香豆素基pH荧光探针合成及性能[J]. 发光学报, 2021,42(4):462-469. DOI： 10.37188/CJL.20200363.

Jun CHEN, Yan FENG, Xiang-ming MENG. Synthesis and Properties of A Mitochondria-targeted Coumarin-based pH Fluorescent Probe[J]. Chinese Journal of Luminescence, 2021,42(4):462-469. DOI： 10.37188/CJL.20200363.

摘要

线粒体作为一种重要的细胞器，其功能包括能量供应、信号传导、细胞分化以及控制细胞生理周期和细胞生长等。线粒体的任何损伤及随后的功能障碍都可能导致一系列人类疾病。其中线粒体pH直接影响着其生化过程，正常的pH水平是线粒体功能正常运作的基础。本文以7-羟基香豆素为原料，合成了一种对线粒体特异性标记的pH荧光探针(CMPH)。并对该探针的光学性质、细胞毒性和线粒体靶向能力进行了研究。结果表明，探针CMPH具有较大的斯托克斯位移，能对pH特异性荧光响应。pH在6.58.2范围内时，探针的荧光强度和pH值具有良好的线性关系。细胞毒性测试表明探针CMPH细胞毒性低。共聚焦荧光成像结果证明，该探针能有效靶向线粒体并能监测线粒体pH的变化。我们的目的是为线粒体pH变化的监测提供一种新的成像工具。

Abstract

Mitochondrion is an important organelle whose functions include energy supply

signal transduction

cell differentiation

cell death

and the control of cell physiological cycle and cell growth. Any damage to mitochondria and subsequent dysfunction could lead to a range of human diseases. Mitochondrial pH directly affects its biochemical process

and the normal pH level is the basis for the normal operation of mitochondrial function. In this paper

a mitochondria-targeted pH fluorescent probe(CMPH) was synthesized by choosing 7-hydroxycoumarin as the raw material. Its optical properties

cytotoxicity and mitochondria-targeted ability were studied. The results showed that probe CMPH had a large Stokes shift

can specifically give a fluorescent response to pH

and its fluorescent intensities had a good linear relationship to pH values in the range of pH=6.5-8.2. Cytotoxicity testing indicated that probe CMPH had low cytotoxicity. Confocal fluorescence imaging demonstrated that CMPH can efficiently target mitochondria and monitor the change of mitochondrial pH. Our purpose is to provide a new imaging tool for monitoring the change of mitochondrial pH.

关键词

荧光探针香豆素pH线粒体

Keywords

fluorescent probecoumarinpHmitochondria

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