一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测

王芹; 刘智峰; 李利华; 黄佩; 孙艳丽; 葛举; 张晟瑞

doi:10.37188/CJL.20220044

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一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测

发光学应用及交叉前沿 | 更新时间：2022-10-19

- 一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测
  增强出版
- A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample
- 发光学报 2022年43卷第6期页码：986-994
- 作者机构：
  
  陕西理工大学化学与环境科学学院，陕西省催化基础与应用重点实验室，陕西汉中　723000
- 作者简介：
  
  [ "王芹（1985‒），女，安徽阜阳人，博士，副教授，2012年于兰州大学获得博士学位，主要从事纳米荧光探针的设计合成及其性能的研究。" ]
  [ "张晟瑞（1985‒），男，甘肃平凉人，博士，教授，2018年于西北大学获得博士学位，主要从事荧光探针的设计合成及其性能的研究。" ]
- 基金信息：
  
  国家自然科学基金(22177066);陕西省创新能力支撑计划(2021KJXX-51);陕西省教育厅科学研究计划(20JK0575);陕西省自然科学基础研究计划(2021JQ-751);秦巴生物资源与生态环境国家重点实验室（培育）“市校共建”科研专项(SXJ-2103;SXJ-2105)
- DOI：10.37188/CJL.20220044
  中图分类号： O657.3
- 纸质出版日期：2022-06-05，
  
  收稿日期：2022-02-10，
  
  修回日期：2022-03-08，
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王芹,刘智峰,李利华等.一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测[J].发光学报,2022,43(06):986-994.

WANG Qin,LIU Zhi-feng,LI Li-hua,et al.A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample[J].Chinese Journal of Luminescence,2022,43(06):986-994.
王芹,刘智峰,李利华等.一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测[J].发光学报,2022,43(06):986-994. DOI： 10.37188/CJL.20220044.

WANG Qin,LIU Zhi-feng,LI Li-hua,et al.A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample[J].Chinese Journal of Luminescence,2022,43(06):986-994. DOI： 10.37188/CJL.20220044.

摘要

苯硫酚（Thiophenol，PhSH）是一种剧毒物质，对环境和健康危害极大，因此，对环境中PhSH的快速检测具有重要意义。本工作中，碳点基纳米探针（

CD-DNS

）以2，4-二硝基苯磺酰胺为识别基团，实现了环境样品中PhSH的高选择性检测。探针

CD-DNS

溶液本身的荧光强度较弱，加入PhSH后，

CD-DNS

与其发生亲核取代反应，2，4-二硝基苯磺酰胺键断裂，释放出荧光团，导致探针的荧光强度和吸光度显著增强。进一步研究表明，

CD-DNS

在520 nm处的荧光强度和440 nm处的吸光度随着PhSH浓度的增加呈线性增加，线性范围为0~5 μmol/L。该探针对PhSH的检测具有反应速度快（4 min）、选择性好等特点。此外，

CD-DNS

可用于环境水样中PhSH的检测，且可制备成试纸条实现PhSH的半定量可视化检测。实验结果表明，碳点基纳米探针

CD-DNS

在环境领域具有广阔的潜在应用前景。

Abstract

Thiophenol（PhSH） is a highly toxic substance， which does great harm to environment and health. Therefore

the rapid detection of PHSH in the environment is of great significance. In this work， the carbon dot（CD）-based nanoprobe

CD-DNS

used 2，4-dinitrobenzenesulfonyl as the recognition group was performed to achieve a highly selective detection of PhSH in the environmental samples. The CD-based nanoprobe

CD-DNS

solution exhibited very weak fluorescence and absorbance. After adding PhSH， the 2，4-dinitrobenesulfonamide moiety of

CD-DNS

could be readily cleaved

via

nucleophilic substitution reaction， leading to a dramatic increase of fluorescence intensity and absorbance of the probe. Further studies showed that the fluorescence intensity at 520 nm and absorbance at 440 nm of the probe showed a good linear relationship with the PhSH concentration， with a linear range of 0-5 μmol/L. The established detection probe displayed fast reaction（4 min） and high selectivity towards PhSH. In addition，

CD-DNS

could be used for the detection of PhSH in environmental water samples， and also could be prepared into a strip to realize the semi-quantitative visual detection of PhSH. The experimental results show that the CD-based nanoprobe

CD-DNS

has a potential application prospect in the environmental field.

关键词

碳点纳米探针比色/荧光增强型苯硫酚环境样品

Keywords

carbon dotsnanoprobecolorimetric/fluorescent turn-onthiophenolenvironmental samples

references

SUN Q，YANG S H，WU L，et al. A highly sensitive and selective fluorescent probe for thiophenol designed via a twist-blockage strategy ［J］. Anal. Chem.， 2016，88（4）：2266-2272. doi: 10.1021/acs.analchem.5b04029http://dx.doi.org/10.1021/acs.analchem.5b04029

WANG T W，CHAMBERLAIN E，SHI H L，et al. Identification of hydrolytic metabolites of dyfonate in alkaline aqueous solutions by using high performance liquid chromatography-UV detection and gas chromatography-mass spectrometry ［J］. Int. J. Environ. Anal. Chem.， 2010，90（12）：948-961. doi: 10.1080/03067310902874889http://dx.doi.org/10.1080/03067310902874889

HE J X，AKAO T，TANI T. Development of a simple HPLC method for determination of paeoniflorin-metabolizing activity of intestinal bacteria in rat feces ［J］. Chem. Pharm. Bull.， 2002，50（9）：1233-1237. doi: 10.1248/cpb.50.1233http://dx.doi.org/10.1248/cpb.50.1233

刘红文，朱隆民，娄霄峰，等. 用于细胞和组织中弗林蛋白酶特异性成像的双光子荧光探针研究［J］. 化学学报，2020，78（11）：1240-1245. doi: 10.6023/A20070323http://dx.doi.org/10.6023/A20070323

LIU H W，ZHU L M，LOU X F，et al. A two-photon fluorescent probe for specific imaging of furin activity in living cells and tissues ［J］. Acta Chim. Sinica， 2020，78（11）：1240-1245. （in Chinese）. doi: 10.6023/A20070323http://dx.doi.org/10.6023/A20070323

罗凡，郑东滨，阳志强，等. 新型姜黄素衍生物荧光探针的合成及对半胱氨酸的选择性识别［J］. 发光学报， 2021，42（11）：1810-1817. doi: 10.37188/CJL.20210211http://dx.doi.org/10.37188/CJL.20210211

LUO F，ZHENG D B，YANG Z Q，et al. A novel curcumin derivatives fluorescent probe for selective recognition of cysteine ［J］. Chin. J. Lumin.， 2021，42（11）：1810-1817. （in Chinese）. doi: 10.37188/CJL.20210211http://dx.doi.org/10.37188/CJL.20210211

SHAO X M，KANG R X，ZHANG Y L，et al. Highly selective and sensitive 1-amino BODIPY-based red fluorescent probe for thiophenols with high off-to-on contrast ratio ［J］. Anal. Chem.， 2015，87（1）：399-405. doi: 10.1021/ac5028947http://dx.doi.org/10.1021/ac5028947

JIANG W，FU Q Q，FAN H Y，et al. A highly selective fluorescent probe for thiophenols ［J］. Angew. Chem. Int. Ed.， 2007，46（44）：8445-8448. doi: 10.1002/anie.200702271http://dx.doi.org/10.1002/anie.200702271

ZHANG S R，WANG Q，WU F F，et al. Merocyanine-based turn-on fluorescent probe for the sensitive and selective determination of thiophenols via a pKa shift mechanism ［J］. Talanta， 2020，216：120965-1-7. doi: 10.1016/j.talanta.2020.120965http://dx.doi.org/10.1016/j.talanta.2020.120965

WU J J，YE Z，WU F，et al. A rhodamine-based fluorescent probe for colorimetric and fluorescence lighting-up determination of toxic thiophenols in environmental water and living cells ［J］. Talanta， 2018，181：239-247. doi: 10.1016/j.talanta.2018.01.028http://dx.doi.org/10.1016/j.talanta.2018.01.028

PERUMAL S，ATCHUDAN R，EDISON T N J I，et al. Sustainable synthesis of multifunctional carbon dots using biomass and their applications：a mini-review ［J］. J. Environ. Chem. Eng.， 2021，9（4）：105802. doi: 10.1016/j.jece.2021.105802http://dx.doi.org/10.1016/j.jece.2021.105802

WAREING T C，GENTILE P，PHAN A N. Biomass-based carbon dots：current development and future perspectives ［J］. ACS Nano， 2021，15（10）：15471-15501. doi: 10.1021/acsnano.1c03886http://dx.doi.org/10.1021/acsnano.1c03886

陈丽娟，刘仁勇，赵丹，等. 聚乙烯亚胺修饰的碳点荧光探针用于检测汞离子［J］. 分析化学， 2020，48（8）：1067-1074. doi: 10.19756/j.issn.0253-3820.191528http://dx.doi.org/10.19756/j.issn.0253-3820.191528

CHEN L J，LIU R Y，ZHAO D，et al. A novel fluorescent probe for mercury ion detection based on polyethyleneimine modified carbon dots ［J］. Chin. J. Anal. Chem.， 2020，48（8）：1067-1074. （in Chinese）. doi: 10.19756/j.issn.0253-3820.191528http://dx.doi.org/10.19756/j.issn.0253-3820.191528

刘俊，张熙荣，熊焕明. 荧光碳点在指纹检测中的应用［J］. 发光学报， 2021，42（8）：1095-1113. doi: 10.37188/CJL.20200334http://dx.doi.org/10.37188/CJL.20200334

LIU J，ZHANG X R，XIONG H M. Application of fluorescent carbon dots in fingerprint detection ［J］. Chin. J. Lumin.， 2021，42（8）：1095-1113. （in Chinese）. doi: 10.37188/CJL.20200334http://dx.doi.org/10.37188/CJL.20200334

宋金萍，马琦，梁晓敏，等. 高量子产率P，N掺杂碳点用于苦味酸检测及荧光上染研究［J］. 分析试验室， 2021，40（11）：1265-1268.

SONG J P，MA Q，LIANG X M，et al. P，N-doped carbon dots with high quantum yield for the detection of picric acid and fluorescent dying study ［J］. Chin. J. Anal. Lab.， 2021，40（11）：1265-1268. （in Chinese）

李庆芝，周奕华，陈袁，等. 比率型碳点荧光传感器检测机理与应用研究进展［J］. 发光学报，2020，41（5）：579-591. doi: 10.3788/fgxb20204105.0579http://dx.doi.org/10.3788/fgxb20204105.0579

LI Q Z，ZHOU Y H，CHEN Y，et al. Research progress on detection mechanism and application of carbon dots-based ratiometric fluorescence sensor ［J］. Chin. J. Lumin.， 2020，41（5）：579-591. （in Chinese）. doi: 10.3788/fgxb20204105.0579http://dx.doi.org/10.3788/fgxb20204105.0579

张震，曲丹，安丽，等. 荧光碳点的制备、发光机理及应用［J］. 发光学报， 2021，42（8）：1125-1140. doi: 10.37188/CJL.20210061http://dx.doi.org/10.37188/CJL.20210061

ZHANG Z，QU D，AN L，et al. Preparation，luminescence mechanism and application of fluorescent carbon dots ［J］. Chin. J. Lumin.， 2021，42（8）：1125-1140. （in Chinese）. doi: 10.37188/CJL.20210061http://dx.doi.org/10.37188/CJL.20210061

南福春，薛小矿，葛介超，等. 红光/近红外光响应碳点在肿瘤治疗中的应用进展［J］. 发光学报， 2021，42（8）：1155-1171. doi: 10.37188/CJL.20210163http://dx.doi.org/10.37188/CJL.20210163

NAN F C，XUE X K，GE J C，et al. Recent advances of red/near infrared light responsive carbon dots for tumor therapy ［J］. Chin. J. Lumin.， 2021，42（8）：1155-1171. （in Chinese）. doi: 10.37188/CJL.20210163http://dx.doi.org/10.37188/CJL.20210163

WANG Q，ZHANG S R，ZHONG Y G，et al. Preparation of yellow-green-emissive carbon dots and their application in constructing a fluorescent turn-on nanoprobe for imaging of selenol in living cells ［J］. Anal. Chem.， 2017，89（3）：1734-1741. doi: 10.1021/acs.analchem.6b03983http://dx.doi.org/10.1021/acs.analchem.6b03983

WU Y Q，SHI A P，LIU H Y，et al. A novel near-infrared xanthene-based fluorescent probe for detection of thiophenol in vitro and in vivo ［J］. New J. Chem.， 2020，44（40）：17360-17367. doi: 10.1039/d0nj03370ghttp://dx.doi.org/10.1039/d0nj03370g

WU Q Q，WANG J B，LIANG W L. A red-to-near-infrared fluorescent probe for the detection of thiophenol based on a novel hydroxylflavone-quinoline-amino molecular system with large Stokes shift ［J］. Dyes Pigm.， 2021，190：109289-1-8. doi: 10.1016/j.dyepig.2021.109289http://dx.doi.org/10.1016/j.dyepig.2021.109289

XU T，ZHAO S J，WU X L，et al. β-cyclodextrin-promoted colorimetric and fluorescence turn-on probe for discriminating highly toxic thiophenol from biothiols ［J］. ACS Sustainable Chem. Eng.， 2020，8（16）：6413-6421. doi: 10.1021/acssuschemeng.0c00766http://dx.doi.org/10.1021/acssuschemeng.0c00766

张改清，阴彩霞. 基于二氰基异佛尔酮的荧光探针在检测苯硫酚中的应用［J］. 无机化学学报， 2021，37（7）：1245-1250. doi: 10.11862/CJIC.2021.161http://dx.doi.org/10.11862/CJIC.2021.161

ZHANG G Q，YIN C X. Application of fluorescent probe based on dicyanoisophorone in detection of thiophenol ［J］. Chin. J. Inorg. Chem.， 2021，37（7）：1245-1250. （in Chinese）. doi: 10.11862/CJIC.2021.161http://dx.doi.org/10.11862/CJIC.2021.161

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