低分子量聚乙烯亚胺/金纳米棒纳米载体的制备及毒性研究

王倩; 刘丽炜; 朱泠西; 邹鹏; 胡思怡; 卜承飞

doi:10.3788/fgxb20153611.1271

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低分子量聚乙烯亚胺/金纳米棒纳米载体的制备及毒性研究

材料合成及性能 | 更新时间：2020-08-12

- 低分子量聚乙烯亚胺/金纳米棒纳米载体的制备及毒性研究
- Synthesization and Toxicity of Low-molecular-weight PEI/Gold Nanords Nanosystem
- 发光学报 2015年36卷第11期页码：1271-1277
- 作者机构：
  
  长春理工大学理学院国际纳米光子学与生物光子学联合研究中心,吉林长春,130022
- 作者简介：
- 基金信息：
  
  国家自然科学基金(11204020)();吉林省国际纳米光子学与生物光子学重点实验室支撑项目(20140622009JC)();总装备部预研基金(62201070711
- DOI：10.3788/fgxb20153611.1271
  中图分类号： O482.31
- 纸质出版日期：2015-11-10，
  
  收稿日期：2015-5-25，
  
  修回日期：2015-9-28，
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王倩, 刘丽炜, 朱泠西等. 低分子量聚乙烯亚胺/金纳米棒纳米载体的制备及毒性研究[J]. 发光学报, 2015,36(11): 1271-1277

WANG Qian, LIU Li-wei, ZHU Ling-xi etc. Synthesization and Toxicity of Low-molecular-weight PEI/Gold Nanords Nanosystem[J]. Chinese Journal of Luminescence, 2015,36(11): 1271-1277
王倩, 刘丽炜, 朱泠西等. 低分子量聚乙烯亚胺/金纳米棒纳米载体的制备及毒性研究[J]. 发光学报, 2015,36(11): 1271-1277 DOI： 10.3788/fgxb20153611.1271.

WANG Qian, LIU Li-wei, ZHU Ling-xi etc. Synthesization and Toxicity of Low-molecular-weight PEI/Gold Nanords Nanosystem[J]. Chinese Journal of Luminescence, 2015,36(11): 1271-1277 DOI： 10.3788/fgxb20153611.1271.

摘要

以表面修饰巯基十一烷酸的金纳米棒 (GNRs/MUA)为骨架

将低分子量的聚乙烯亚胺(PEI)连接到GNRs/MUA表面

构建GNRs/MUA/PEI纳米载体.首先采用MUA对GNRs进行表面修饰

减少由于GNRs表面的十六烷基三甲基溴化铵(CTAB)所造成的生物毒性.然后采用低分子量 PEI 进一步修饰

同时利用GNRs巨大的比表面积进一步放大 PEI 的携带基因能力

这样既能够降低阳离子聚合物的毒性

又能够提高整个体系的转染效率.利用透射电子显微镜(TEM)、紫外可见吸收光谱(UV-Vis)、Zeta电位等对纳米载体进行了表征.结果显示

MUA与PEI已成功修饰到GNRs表面

并很好地保留了GNRs的光学性质

其表面电位发生正负交替变化.采用噻唑蓝(MTT)比色法对纳米载体进行细胞毒性研究

结果显示GNRs/MUA/PEI(1.8 kDa)非病毒纳米载体

细胞存活率在控制聚合物浓度为300 g/mL时仍然稳定在75%以上

明显高于商品化的PEI(25 kDa).

Abstract

Based on the gold nanorods modified by 11-mercaptopropanic acid (MUA) as the skeleton

the low molecular weight polyethylenimine PEI was conjugated to the surface of GNRs/MUA to form GNRs/MUA/PEI nanocomplexes. Firstly

surface modification of gold nanorods was carried out by using MUA to reduce the toxicity of gold nanorods caused by CTAB surfactants

and then further modified by low molecular weight PEI. The large surface area of gold nanorods allows them to carry more genes

which can simultaneously reduce the toxicity of cationic polymer and improve the transfection efficiency of the whole system. GNRs/MUA/PEI were characterized by transmission electron microscopy (TEM)

UV-Vis absorption spectra

and Zeta potential. The results show that MUA and PEI are conjugated to GNRs successfully which cause charge reversal on the surface of GNRs

and the optical properties of GNRs are well preserved.

In vitro

cytotoxic effects of GNRs/MUA/PEI (1.8 kDa) are quantified by MTT assay

and the results suggest that the cell viability is above 75% at the concentration of 300 g/mL

much higher than the commercialized PEI(25 kDa).

关键词

金纳米棒聚乙烯亚胺非病毒载体细胞毒性

Keywords

gold nanorodspolyethyleniminenonviral vectorscytotoxicity

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