WU WEN-BO, ZHANG YUE-CHENG, LI CHENG-JIA, et al. Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe. [J]. Chinese journal of luminescence, 2020, 41(3): 331-338.
DOI:
WU WEN-BO, ZHANG YUE-CHENG, LI CHENG-JIA, et al. Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe. [J]. Chinese journal of luminescence, 2020, 41(3): 331-338. DOI: 10.3788/fgxb20204103.0331.
Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe
Green fluorescent castor carbon quantum dots(CO-CQDs) with excellent fluorescence properties were synthesized by one-step hydrothermal method with natural biomass peeled castor. By combining the as-synthesized CO-CQDs with eosin Y(EY)
a novel ratiometric fluorescence probe CO-CQDs/EY was formed. In the Na
2
HPO
4
-citric acid buffer solution of pH=4.00
under the excitation wavelength of 320 nm
the CO-CQDs/EY complex showed two independent fluorescence emission peaks at 405 nm and 540 nm. The fluorescence signals of 405 nm and 540 nm could be both quenched by Cr(Ⅵ). In the presence of L-ascorbic acid
the fluorescence signal at 540 nm was significantly increased
while the fluorescence intensity at 405 nm still remained unchanged. Therefore
a novel method for L-ascorbic acid analysis was developed based on this fluorescence ratiometric probe CO-CQDs/EY. This ratiometric method showed excellent specificity
sensitivity and accuracy. The optimum conditions of experiment were investigated and the mechanism of interaction was discussed. Under the optimized experimental conditions
the linear range of this method was 5.010
-8
-4.010
-6
mol/L
and the detection limit was calculated to be 3.710
-8
mol/L. Moreover
this method has been successfully applied to L-ascorbic acid analysis in drugs
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