NIE Fang, NIE Ya-kun, WANG Xiao-hui etc. Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide[J]. Chinese Journal of Luminescence, 2020,41(7): 826-833
NIE Fang, NIE Ya-kun, WANG Xiao-hui etc. Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide[J]. Chinese Journal of Luminescence, 2020,41(7): 826-833 DOI: 10.37188/fgxb20204107.0826.
Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide
) is the main marker of reactive oxygen species and closely related to various diseases such as neurodegenerative diseases. In this work
we presented an enzymatic nanosensor for fluorescence sensing of intracellular hydrogen peroxide. The nanosensor consisted of a biocompatible shell of poly-l-lysine and horseradish peroxidase(HRP)
and an oxygen sensing core from a porous polymeric matrix containing oxygen probes. H
2
O
2
was catalyzed by HRP to yield the product of oxygen
which was reported by the sensing core in the form of quenched fluorescence. The enzymatic nanosensors had a hydrodynamic size of about 270 nm
a zeta potential of -18 mV and good biocompatibility. Their fluorescence were highly sensitive to H
2
O
2
in both ratiometric and the time-resolved fluorescence. Moreover
the nanosensors can be efficiently internalized by live cells
thereby intracelluar H
2
O
2
was sensitively detected with TRF modality. These results suggest that our enzymatic nanosensors may be used for monitoring H
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