Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide

NIE Fang; NIE Ya-kun; WANG Xiao-hui; PENG Hong-shang

doi:10.37188/fgxb20204107.0826

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Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide

Device Fabrication and Physics | 更新时间：2020-09-10

- Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide
- Chinese Journal of Luminescence Vol. 41, Issue 7, Pages: 826-833(2020)
- 作者机构：
  
  1. 中央民族大学生命与环境科学学院北京,100081
  2. 中央民族大学理学院北京,100081
  3. 北京邮电大学电子工程学院北京,100876
- 作者简介：
- 基金信息：
  
  Supported by National Natural Science Foundation of China(61775245);MUC 111 Projec
- DOI：10.37188/fgxb20204107.0826
  CLC：
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NIE Fang, NIE Ya-kun, WANG Xiao-hui, et al. Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide. [J]. Chinese Journal of Luminescence 41(7):826-833(2020)
DOI：

NIE Fang, NIE Ya-kun, WANG Xiao-hui, et al. Ratiometric Fluorescent Enzymatic Nanosensors for Intracellular Hydrogen Peroxide. [J]. Chinese Journal of Luminescence 41(7):826-833(2020) DOI： 10.37188/fgxb20204107.0826.

摘要

过氧化氢（H,2,O,2,）是活性氧类的主要标志物，它与多种疾病如神经退行性疾病密切相关。本文设计了一种检测细胞内过氧化氢的荧光过氧化氢酶纳米传感器。这种纳米传感器由含多聚赖氨酸、辣根过氧化物酶的生物相容性外壳和含有氧探针的多孔聚合物基质的氧传感核组成。辣根过氧化物酶（HRP）催化H,2,O,2,生成氧，进而通过荧光氧气探针进行探测。该酶纳米传感器的流体动力学尺寸约为270 nm，zeta电位为-18 mV，具有良好的生物相容性。它的荧光比率和时间分辨荧光均对H,2,O,2,高度敏感。此外，该纳米传感器可以被活细胞有效地摄取，从而可以用TRF 方式灵敏地检测细胞内的H,2,O,2,浓度。结果表明，本实验制备的酶纳米传感器有望进一步用于监测H,2,O,2,相关的细胞生化反应，如氧化应激。

Abstract

Hydrogen peroxide(H,2,O,2,) is the main marker of reactive oxygen species and closely related to various diseases such as neurodegenerative diseases. In this work, we presented an enzymatic nanosensor for fluorescence sensing of intracellular hydrogen peroxide. The nanosensor consisted of a biocompatible shell of poly-l-lysine and horseradish peroxidase(HRP), and an oxygen sensing core from a porous polymeric matrix containing oxygen probes. H,2,O,2, was catalyzed by HRP to yield the product of oxygen, which was reported by the sensing core in the form of quenched fluorescence. The enzymatic nanosensors had a hydrodynamic size of about 270 nm, a zeta potential of -18 mV and good biocompatibility. Their fluorescence were highly sensitive to H,2,O,2, in both ratiometric and the time-resolved fluorescence. Moreover, the nanosensors can be efficiently internalized by live cells, thereby intracelluar H,2,O,2, was sensitively detected with TRF modality. These results suggest that our enzymatic nanosensors may be used for monitoring H,2,O,2,-related cellular events, such as oxidative stress.

关键词

过氧化氢纳米传感器时间分辨荧光比率荧光氧化应激

Keywords

H2O2nanosensortime-resolved fluorescence (TRF)ratiometric fluorescenceoxidative stress

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