Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water

LI Ruxue; HUANG Mingyue; ZHOU Peng; DUAN Chunying

doi:10.37188/CJL.20230133

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Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water

Luminescence Applications and Interdisciplinary Fields | 更新时间：2023-11-01

- Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water
  增强出版
- Chinese Journal of Luminescence Vol. 44, Issue 10, Pages: 1872-1880(2023)
- 作者机构：
  
  大连理工大学化学学院，辽宁大连　116024
- 作者简介：
- 基金信息：
  
  the National Major Scientific Instruments and Equipment Develop Project of China(2012YQ090197);the National Science Foundation of China(52070032)
- DOI：10.37188/CJL.20230133
  CLC： O482.31
- Published：05 October 2023，
  
  Received：17 May 2023，
  
  Revised：04 June 2023，
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李如雪,黄明玥,周硼等.基于双发射碳点的荧光探针构建及对水中铜离子的检测[J].发光学报,2023,44(10):1872-1880.

LI Ruxue,HUANG Mingyue,ZHOU Peng,et al.Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water[J].Chinese Journal of Luminescence,2023,44(10):1872-1880.
李如雪,黄明玥,周硼等.基于双发射碳点的荧光探针构建及对水中铜离子的检测[J].发光学报,2023,44(10):1872-1880. DOI： 10.37188/CJL.20230133.

LI Ruxue,HUANG Mingyue,ZHOU Peng,et al.Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water[J].Chinese Journal of Luminescence,2023,44(10):1872-1880. DOI： 10.37188/CJL.20230133.

摘要

为克服测试时环境变化对荧光检测的影响，我们设计合成了比率型荧光探针，利用双波长的荧光发射来有效消除背景干扰。本文以邻苯二胺、四硼酸钠和1⁃甲基⁃3⁃烯丙基咪唑溴盐为反应前体，利用一步水热法合成了基于碳点双发射的荧光探针L⁃CDs，并实现了对铜离子（Cu

）的双信号响应。L⁃CDs表现出荧光双发射现象，当激发波长为380 nm时，呈现出440 nm和570 nm的双发射峰。Cu

可使探针在440 nm处的荧光发射强度减弱，同时570 nm的荧光发射峰增强。Cu

浓度在0.04~0.244 mmol/L范围内时，与荧光比率信号（

570

440

）表现出良好的线性相关，检出限（LOD）为0.6 μmol/L。所构建的荧光探针可用于实际水样中Cu

的检测，回收率为99.4% ~ 101.8%。

Abstract

In order to overcome the influence of environmental change on fluorescence detection， a ratio fluorescence probe was designed and prepared， and dual-wavelength fluorescence emission was used to effectively eliminate background interference. Using o-phenylenediamine， sodium tetraborate and 1-methyl-3-allyl imidazolium bromide as precursors， a one-step hydrothermal method has been developed to synthesize a ratio fluorescent probe based on carbon dots （L-CDs）， and to achieve a double signal response to heavy metal ions （Cu

）. L-CDs exhibited fluorescence double emission phenomenon， and showed double emission peaks at 440 nm and 570 nm when the excitation wavelength was 380 nm. The addition of Cu

can reduce the fluorescence emission intensity at 440 nm and increase the fluorescence emission peak value at 570 nm. The concentration of Cu

in the range of 0.04-0.244 mmol/L showed a good linear correlation with the fluorescence ratio signal （

570

440

）， and the detection limit （LOD） was 0.6 μmol/L. The fluorescence probe can be used for the detection of Cu

in actual water samples with the recovery of 99.4%-101.4%.

关键词

碳量子点荧光探针铜离子比率探针

Keywords

carbon dotsfluorescence probecupper ionratio probe

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