A Ratiometric Fluorescent Polystyrene Microsphere Hybrid Probe for Highly Selective Detection of Anthrax Markers

HU Runze; XU Chen; FANG Zhou; LI Ying; MIN Hua

doi:10.37188/CJL.20230049

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A Ratiometric Fluorescent Polystyrene Microsphere Hybrid Probe for Highly Selective Detection of Anthrax Markers

Luminescence Applications and Interdisciplinary Fields | 更新时间：2023-09-28

- A Ratiometric Fluorescent Polystyrene Microsphere Hybrid Probe for Highly Selective Detection of Anthrax Markers
  增强出版
- Chinese Journal of Luminescence Vol. 44, Issue 9, Pages: 1693-1704(2023)
- 作者机构：
  
  1.上海理工大学材料与化学学院，上海　200093
  2.上海理工大学科技发展研究院技术转移中心，上海　200093
- 作者简介：
- 基金信息：
  
  国家自然科学基金(21101107;51173107);污染控制与资源化国家重点实验室基金项目(PCRRF19017)
- DOI：10.37188/CJL.20230049
  CLC： TB333
- Published：05 September 2023，
  
  Received：01 March 2023，
  
  Revised：13 March 2023，
- 稿件说明：
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胡润泽,徐陈,方舟等.一种比率型荧光聚苯乙烯微球杂化探针用于炭疽病毒标志物的高选择性检测[J].发光学报,2023,44(09):1693-1704.

HU Runze,XU Chen,FANG Zhou,et al.A Ratiometric Fluorescent Polystyrene Microsphere Hybrid Probe for Highly Selective Detection of Anthrax Markers[J].Chinese Journal of Luminescence,2023,44(09):1693-1704.
胡润泽,徐陈,方舟等.一种比率型荧光聚苯乙烯微球杂化探针用于炭疽病毒标志物的高选择性检测[J].发光学报,2023,44(09):1693-1704. DOI： 10.37188/CJL.20230049.

HU Runze,XU Chen,FANG Zhou,et al.A Ratiometric Fluorescent Polystyrene Microsphere Hybrid Probe for Highly Selective Detection of Anthrax Markers[J].Chinese Journal of Luminescence,2023,44(09):1693-1704. DOI： 10.37188/CJL.20230049.

摘要

采用包埋法将通过二苯甲酰甲烷（DBM）和1⁃10无水邻菲啰啉（Phen）制得的稀土配合物Eu（DBM）

Phen包埋进羧基化聚苯乙烯微球中，再通过配位作用引入镧系发光中心Tb

，获得具有双发射中心的荧光聚苯乙烯微球杂化探针Tb⁃PS@Eu（DBM）

Phen。利用SEM、TEM、FT⁃IR、XPS、UV⁃Vis、PL等表征方法对探针分子的结构和性能进行分析。研究结果表明，Tb⁃PS@Eu（DBM）

Phen具有优异的稳定性、分散性和荧光性能。此外，通过进一步研究探针分子对2，6⁃吡啶二甲酸（DPA）的荧光传感性能，发现DPA能够对Tb⁃PS@Eu（DBM）

Phen的荧光产生明显的增强效果，这可能是由于DPA和聚苯乙烯微球表面的铽离子配位，进而使配体⁃稀土之间的能量传递过程受到影响，从而造成Tb⁃PS@Eu（DBM）

Phen的荧光增强。同时，Tb⁃PS@Eu（DBM）

Phen对DPA具有较强的选择性和抗干扰能力，有望用作检测识别DPA的荧光探针。

Abstract

Eu（DBM）

Phen was firstly encapsulated into carboxylated polystyrene microspheres by the encapsulation method， and then the lanthanide luminescence center Tb

was introduced by coordination to obtain the fluorescent polystyrene microsphere hybrid probe Tb-PS@Eu（DBM）

Phen with dual emission centers. The results indicated that Tb-PS@Eu（DBM）

Phen has excellent stability， dispersibility and fluorescence properties. In addition， by further investigating the fluorescence sensing properties of the probe molecule on 2，6-pyridinedicarboxylic acid （DPA）， it was found that Tb-PS@Eu（DBM）

Phen could produce a significant enhancement with the present of DPA， which might be due to the coordination of terbium ions on the surface of DPA and polystyrene microspheres， which in turn affected the energy transfer process between the ligand-rare earths， resulting in Tb-PS@Eu-（DBM）

Phen's fluorescence enhancement. Meanwhile， Tb-PS@Eu（DBM）

Phen has strong selectivity and anti-interference ability for DPA， which is expected to be used as a potential fluorescent probe for the recognition of DPA.

关键词

稀土配合物荧光微球DPA比率荧光传感

Keywords

lanthanide complexesfluorescent microspheresDPAratiometric fluorescent sensing

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