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Microwave Preparation of Common Lophatherum Herb Carbon Quantum Dots and Application in Cell Imaging
增强出版- Chinese Journal of Luminescence Vol. 43, Issue 12, Pages: 2001-2013(2022)
- 作者机构：
1.南京林业大学 材料科学与工程学院， 江苏 南京 210000
2.南京林业大学 江苏省林产品高效加工利用联合创新中心， 江苏 南京 210000
3.南京林业大学 绿色生物质燃料与化学品江苏省重点实验室， 江苏 南京 210000
4.2019年江苏省研究生工作站：靖江国林木业有限公司 （工作站编号：2019_099）， 江苏 靖江 214500
- 作者简介：
- 基金信息：National Natural Science Foundation of China(32071703)
DOI：10.37188/CJL.20220256
CLC： TB383.1Published：05 December 2022，
Received：04 July 2022，
Revised：25 July 2022，
扫 描 看 全 文
胡妙言,刘凯,高诗雨等.淡竹叶碳量子点的微波法制备及在细胞成像中的应用探究[J].发光学报,2022,43(12):2001-2019.
HU Miao-yan,LIU Kai,GAO Shi-yu,et al.Microwave Preparation of Common Lophatherum Herb Carbon Quantum Dots and Application in Cell Imaging[J].Chinese Journal of Luminescence,2022,43(12):2001-2019.
胡妙言,刘凯,高诗雨等.淡竹叶碳量子点的微波法制备及在细胞成像中的应用探究[J].发光学报,2022,43(12):2001-2019. DOI： 10.37188/CJL.20220256.
HU Miao-yan,LIU Kai,GAO Shi-yu,et al.Microwave Preparation of Common Lophatherum Herb Carbon Quantum Dots and Application in Cell Imaging[J].Chinese Journal of Luminescence,2022,43(12):2001-2019. DOI： 10.37188/CJL.20220256.
摘要
利用响应曲面法 （RSM）系统研究了微波作用时间 （
T
）、微波功率 （
W
）、淡竹叶与去离子水的料液比 （
R
）对微波法制备淡竹叶氮硅自掺杂碳量子点 （N/Si⁃CQDs）荧光量子产率 （QY）的影响。得到了上述工艺参数对QY的影响显著性次序以及对应的QY回归模型与最佳工艺参数，通过验证实验证明优化结果可靠。采用最佳工艺得到的N/Si⁃CQDs的平均粒径较小且分布均匀，在水中分散性良好，具有激发依赖的发射特性，荧光稳定性较高，对HEK293细胞具有较低细胞毒性，且可被细胞吸收而照亮细胞从而明确区分细胞质和细胞核，说明该碳量子点可用于细胞成像。该研究不仅为淡竹叶的高值化利用提供了一个新思路，而且对提高生物质碳量子点的微波法制备效率、促进其在细胞成像等生物医学领域的应用具有参考价值。
Abstract
In this study， response surface methodology（RSM） was used to study the effects of microwave action time （
T
）， microwave power（
W
） and the ratio of common lophatherum herb to deionized water （
R
）
on the fluorescence quantum yield （QY） of N/Si-CQDs prepared by microwave method. The significance order of the influence of the above process parameters on QY， and the corresponding QY regression model and the best process parameters are obtained， and the optimization results are proved to be reliable through validation experiments. The average particle size of N/Si-CQDs obtained by the best process is small and evenly distributed， with good dispersion in water， excitation dependent emission characteristics， high fluorescence stability， low cytotoxicity to HEK293 cells. And the N/Si CQDs can be absorbed by cells to illuminate cells， so as to clearly distinguish cytoplasm and nucleus， which shows that the N/Si-CQDs can be used for cell imaging. This study not only provides a new idea for the high-value utilization of bamboo leaves， but also has reference value for improving the efficiency of microwave preparation of biomass carbon quantum dots and promoting their application in biomedical fields such as cell imaging.
关键词
淡竹叶碳量子点微波法响应曲面法细胞成像
Keywords
common lophatherum herbcarbon quantum dotsmicrowave methodresponse surface methodcell imaging
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