A Novel Near-infrared Fluorescent Probe for Detection of Aβ40 Aggregates

XU Yun-ze; YAO Yu-si; LYU Guang-lei; LI Chun-xia

doi:10.37188/CJL.20220228

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A Novel Near-infrared Fluorescent Probe for Detection of Aβ40 Aggregates

Luminescence Applications and Interdisciplinary Fields | 更新时间：2023-10-27

- A Novel Near-infrared Fluorescent Probe for Detection of Aβ40 Aggregates
  增强出版
- Chinese Journal of Luminescence Vol. 43, Issue 10, Pages: 1620-1627(2022)
- 作者机构：
  
  1.山东大学前沿交叉科学青岛研究院，分子科学与工程研究院，山东青岛　266237
  2.浙江师范大学化学与生命科学学院，浙江金华　321004
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(51872263);Zhejiang Provincial Natural Science Foundation of China(LZ19E020001);Taishan Scholars Project(LQ19B050003)
- DOI：10.37188/CJL.20220228
  CLC： TP394.1;TH691.9
- Published：05 October 2022，
  
  Received：05 June 2022，
  
  Revised：18 June 2022，
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徐蕴泽,尧雨斯,吕光磊等.一种新型近红外荧光探针用于Aβ40聚集体的检测[J].发光学报,2022,43(10):1620-162710.37188/CJL.20220228.

XU Yun-ze,YAO Yu-si,LYU Guang-lei,et al.A Novel Near-infrared Fluorescent Probe for Detection of Aβ40 Aggregates[J].Chinese Journal of Luminescence,2022,43(10):1620-162710.37188/CJL.20220228.
徐蕴泽,尧雨斯,吕光磊等.一种新型近红外荧光探针用于Aβ40聚集体的检测[J].发光学报,2022,43(10):1620-162710.37188/CJL.20220228. DOI：

XU Yun-ze,YAO Yu-si,LYU Guang-lei,et al.A Novel Near-infrared Fluorescent Probe for Detection of Aβ40 Aggregates[J].Chinese Journal of Luminescence,2022,43(10):1620-162710.37188/CJL.20220228. DOI：

摘要

β⁃淀粉样蛋白（Aβ）形成的老年斑块是阿尔茨海默症（Alzheimer's disease，AD）最重要的病理性标志物之一，在AD的发病中起到了非常重要的作用。在AD的发病前期，Aβ40聚集体是最为主要且大量存在的蛋白形式。因此，对Aβ40聚集体的检测有利于实现对AD的早期诊断。本工作设计合成了一种具有D⁃π⁃A结构的小分子近红外荧光探针

⁃

用于Aβ40聚集体的荧光检测。探针

⁃

具有良好的荧光发射波长（

=640 nm），对Aβ40聚集体表现出了较好的结合能力（

=3.072 μmol/L），能够实现在溶液中对Aβ40聚集体的特异性检测。除此之外，该探针合成简单，成本低廉，灵敏度较高，响应速度极快。

Abstract

β-Amyloid（Aβ） senile plaques are one of the most important pathological markers of Alzheimer's disease（AD）， which plays a quite important role in the pathogenesis of AD. In the early stage of AD， Aβ40 aggregates are the most predominant and abundant. Therefore， the detection of Aβ40 aggregates is conducive to the early diagnosis of AD. In this work， a small molecule fluorescent probe

with a D-π-A structure was designed and synthesized for the fluorescence detection of Aβ40 aggregates. The probe

displays superior fluorescence properties（

=640 nm） and shows remarkable binding capacity for Aβ40 aggregates（

=3.072 μmol/L）， enabling the specific detection of Aβ40 aggregates in solution. In addition， the probe has series of advantages， including simple synthesis， low cost， high sensitivity and fast response.

关键词

荧光探针Aβ阿尔茨海默症近红外

Keywords

fluorescent probeAβAlzheimer's diseasenear-infrared （NIR）

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