A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample

WANG Qin; LIU Zhi-feng; LI Li-hua; HUANG Pei; SUN Yan-li; GE Ju; ZHANG Sheng-rui

doi:10.37188/CJL.20220044

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A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample

Luminescence Applications and Interdisciplinary Fields | 更新时间：2022-10-19

- A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample
  增强出版
- Chinese Journal of Luminescence Vol. 43, Issue 6, Pages: 986-994(2022)
- 作者机构：
  
  陕西理工大学化学与环境科学学院，陕西省催化基础与应用重点实验室，陕西汉中　723000
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China(22177066);Innovation Capability Support Program of Shaanxi(2021KJXX-51);Scientific Research Program Funded by Education Department of Shaanxi Provincial Government(20JK0575);Natural Science Foundation of Shaanxi Province(2021JQ-751);Co-construction Project of Hanzhong city and Shaanxi University of Technology(SXJ-2103;SXJ-2105)
- DOI：10.37188/CJL.20220044
  CLC： O657.3
- Received：10 February 2022，
  
  Revised：08 March 2022，
  
  Published：05 June 2022
- 稿件说明：
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王芹,刘智峰,李利华等.一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测[J].发光学报,2022,43(06):986-994.

WANG Qin,LIU Zhi-feng,LI Li-hua,et al.A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample[J].Chinese Journal of Luminescence,2022,43(06):986-994.
王芹,刘智峰,李利华等.一种比色/荧光增强型碳点基纳米探针用于环境中苯硫酚的高选择性检测[J].发光学报,2022,43(06):986-994. DOI： 10.37188/CJL.20220044.

WANG Qin,LIU Zhi-feng,LI Li-hua,et al.A Colorimetric/Fluorescent-enhanced Carbon Dots Based Nanoprobe for Highly Selective Detection of Thiophenol in Environmental Sample[J].Chinese Journal of Luminescence,2022,43(06):986-994. DOI： 10.37188/CJL.20220044.

摘要

苯硫酚（Thiophenol，PhSH）是一种剧毒物质，对环境和健康危害极大，因此，对环境中PhSH的快速检测具有重要意义。本工作中，碳点基纳米探针（

CD-DNS

）以2，4-二硝基苯磺酰胺为识别基团，实现了环境样品中PhSH的高选择性检测。探针

CD-DNS

溶液本身的荧光强度较弱，加入PhSH后，

CD-DNS

与其发生亲核取代反应，2，4-二硝基苯磺酰胺键断裂，释放出荧光团，导致探针的荧光强度和吸光度显著增强。进一步研究表明，

CD-DNS

在520 nm处的荧光强度和440 nm处的吸光度随着PhSH浓度的增加呈线性增加，线性范围为0~5 μmol/L。该探针对PhSH的检测具有反应速度快（4 min）、选择性好等特点。此外，

CD-DNS

可用于环境水样中PhSH的检测，且可制备成试纸条实现PhSH的半定量可视化检测。实验结果表明，碳点基纳米探针

CD-DNS

在环境领域具有广阔的潜在应用前景。

Abstract

Thiophenol（PhSH） is a highly toxic substance， which does great harm to environment and health. Therefore

the rapid detection of PHSH in the environment is of great significance. In this work， the carbon dot（CD）-based nanoprobe

CD-DNS

used 2，4-dinitrobenzenesulfonyl as the recognition group was performed to achieve a highly selective detection of PhSH in the environmental samples. The CD-based nanoprobe

CD-DNS

solution exhibited very weak fluorescence and absorbance. After adding PhSH， the 2，4-dinitrobenesulfonamide moiety of

CD-DNS

could be readily cleaved

via

nucleophilic substitution reaction， leading to a dramatic increase of fluorescence intensity and absorbance of the probe. Further studies showed that the fluorescence intensity at 520 nm and absorbance at 440 nm of the probe showed a good linear relationship with the PhSH concentration， with a linear range of 0-5 μmol/L. The established detection probe displayed fast reaction（4 min） and high selectivity towards PhSH. In addition，

CD-DNS

could be used for the detection of PhSH in environmental water samples， and also could be prepared into a strip to realize the semi-quantitative visual detection of PhSH. The experimental results show that the CD-based nanoprobe

CD-DNS

has a potential application prospect in the environmental field.

关键词

Keywords

references

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