Orthotopic Bladder Tumor Targeted Carbon Dots for Fluorescence Imaging-guided Phototherapy

Fu-chun NAN; Yang YANG; Xiao-zhi ZHAO; Jie-chao GE; Wei-min LIU; Hao-hui REN; Hong-qian GUO; Peng-fei WANG

doi:10.37188/CJL.20220017

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Orthotopic Bladder Tumor Targeted Carbon Dots for Fluorescence Imaging-guided Phototherapy

Luminescence Applications and Interdisciplinary Fields | 更新时间：2022-04-20

- Orthotopic Bladder Tumor Targeted Carbon Dots for Fluorescence Imaging-guided Phototherapy
  增强出版
- 发光学报 Vol. 43, Issue 4, Pages: 608-619(2022)
- 作者机构：
  
  1.中国科学院理化技术研究所　光化学转换与功能材料重点实验室，北京　100190
  2.中国科学院大学　未来技术学院，北京　100049
  3.南京大学医学院附属鼓楼医院　泌尿外科(南京大学泌尿外科学研究所)，江苏　南京　210008
- 作者简介：
  
  南福春(1992-)，男，山东潍坊人，博士研究生，2017年于青岛科技大学获得硕士学位，主要从事新型碳纳米材料在肿瘤诊疗中的研究。E-mail: nanfuchun@mail.ipc.ac.cn
  葛介超(1970-)，男，山东临沂人，博士，研究员，2008年于山东师范大学获得博士学位，主要从事新型光响应纳米材料的设计及其在抗肿瘤、病毒、细菌、炎症或创伤修复等领域应用的研究。E-mail: jchge2010@mail.ipc.ac.cn
  郭宏骞(1965-)，男，吉林长春人，博士，教授，1999年于北京大学获得博士学位，主要从事泌尿生殖系肿瘤、肾移植、微创泌尿外科的治疗研究。E-mail: dr.ghq@nju.edu.cn
- 基金信息：
  
  国家自然科学基金(51972315)
- DOI：10.37188/CJL.20220017
  CLC： O482.31
- Received：14 January 2022，
  
  Revised：2022-01-30，
  
  Published：01 April 2022
- 稿件说明：
移动端阅览
Fu-chun NAN, Yang YANG, Xiao-zhi ZHAO, et al. Orthotopic Bladder Tumor Targeted Carbon Dots for Fluorescence Imaging-guided Phototherapy[J]. 发光学报, 2022, 43(4): 608-619.
DOI：

Fu-chun NAN, Yang YANG, Xiao-zhi ZHAO, et al. Orthotopic Bladder Tumor Targeted Carbon Dots for Fluorescence Imaging-guided Phototherapy[J]. 发光学报, 2022, 43(4): 608-619. DOI： 10.37188/CJL.20220017.

摘要

膀胱癌是泌尿系统中最常见的肿瘤之一。目前，化疗与手术切除等治疗手段不能有效治愈该肿瘤，并且上述两种治疗方式会产生副作用，降低患者的生活质量。光疗则为膀胱癌提供了一种新的治疗手段。在本工作中，以靶向膀胱癌细胞的小肽PLZ4修饰多功能碳点，得到了兼具靶向膀胱癌细胞及光动力/光热治疗功能的PLZ-4碳点(PCDs)。细胞实验表明该PCDs能够用于膀胱癌细胞靶向成像，并且在635 nm激光照射下，能高效杀灭膀胱癌细胞。活体实验表明经过尾静脉注射后，PCDs能够有效地聚集在原位膀胱癌处，在635 nm激光照射下，原位膀胱癌能够被完全清除，表明该PCDs在荧光成像介导的光动力/光热治疗原位膀胱癌中有潜在的应用价值。

Abstract

Bladder cancer has become one of the most frequent malignant tumors in the urinary system. Chemotherapy and radical cystectomy could not effectively cure this illness and even lead to side effects and low-quality life

which seriously threaten the lives of patients. Phototherapy provides a new method for bladder cancer treatment. Here

multifunctional carbon dots theranostics was developed and modified by a bladder cancer-specific peptide(PLZ4). PLZ4-carbon dots(PCDs) integrate targeted delivery

photodynamic therapy(PDT)

and photothermal therapy(PTT) in one procedure.

In vitro

fluorescence imaging revealed that PCDs tend to aggregate in tumor cells than in normal cells

thus allowing them to effectively ablate MB-49 bladder cancer cells under a 635 nm laser.

In vivo

biodistribution showed that the PCDs specifically accumulated in bladder cancer tissues but exhibited negligible fluorescence in normal bladder tissues. After combined PDT/PTT

the orthotopic bladder tumor can be completely killed

thereby proving that PCDs have a potential as a candidate for bladder cancer treatment.

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Keywords

references

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Related Author

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Fuchun NAN

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Related Institution

a］ Key Laboratory of Photochemical Conversion and Optoelectronic Materials， Technical Institute of Physics and Chemistry， Chinese Academy of Sciences， Beijing， 100190， P. R. China［b］ School of Future Technology， University of Chinese Academy of Sciences， Beijing， 100049， China［c］ Department of Urology， Drum Tower Hospital， Medical School of Nanjing University， Institute of Urology， Nanjing University

中国科学院大学未来技术学院

南京大学医学院附属鼓楼医院泌尿外科（南京大学泌尿外科学研究所）

Institute of Nanochemistry and Nanobiology， Shanghai University

Department of Chemistry， College of Sciences， Shanghai University

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