Fluorescent Detection for Cysteine/Homocysteine Using Hg<sup>2+</sup> Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

Lu LU; Lü XU; Xin ZHANG; Xiao-hong ZHOU; Le-ping YU

doi:10.37188/CJL.20210389

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Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

Luminescence Applications and Interdisciplinary Fields | 更新时间：2022-04-07

- Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots
  增强出版
- Chinese Journal of Luminescence Vol. 43, Issue 3, Pages: 440-452(2022)
- 作者机构：
  
  1.无锡商业职业技术学院　汽车技术学院，江苏　无锡　214153
  2.盐城工学院　材料科学与工程学院，江苏　盐城　224002
- 作者简介：
- 基金信息：
  
  Jiangsu Province Natural Science Foundation(BK20191041);Natural Science Foundation of the Jiangsu Higher Education Institutions of China(19KJB480011);Science Foundation of Wuxi Vocational Institute of Commerce school project(KJXJ21604);Production and Education Integration Project of Wuxi Vocational Institute of Commerce school project(XQ202113)
- DOI：10.37188/CJL.20210389
  CLC： O482.31
- Received：14 December 2021，
  
  Revised：29 December 2021，
  
  Published：2022-03
- 稿件说明：
移动端阅览
Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots[J]. Chinese journal of luminescence, 2022, 43(3): 440-452.
DOI：

Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots[J]. Chinese journal of luminescence, 2022, 43(3): 440-452. DOI： 10.37188/CJL.20210389.

摘要

以叶酸和N-(膦酰基甲基)亚氨基二乙酸为原料，制备了具有丰富氮含量(10.0%)和高荧光量子产率(23.64%)的N

P-CDs。由于Hg

与N

P-CDs之间的电子转移作用，N

P-CDs的荧光将被猝灭，基于此构建了半胱氨酸/同型半胱氨酸(Cys/Hcy)增强型纳米探针。利用Hg

和硫醇基团之间更强的相互作用，实现Cys/Hcy的检测。Cys的测定在0.1~150 μmol/L范围内存在线性关系，Hcy的测定在0.1~100 μmol/L范围内存在线性关系，检测限分别为30 nmol/L和50 nmol/L。此外，本文所制备的N

P-CDs具有较强的抗基质干扰能力，可用于自来水和尿液等真实样品检测；因其超低的细胞毒性和出色的细胞渗透性，可用于检测活细胞内的Hg

/Cys，在生物传感方面具有较为广阔的应用前景。

Abstract

A new kind of N

P-CDs with rich nitrogen content(10.0%) and high fluorescence quantum yield(23.64%) was successfully prepared using folic acid and N-(phosphonomethyl)iminodiacetic acid as precursors. Due to the charge transfer between Hg

and N

P-CDs

the fluorescence of N

P-CDs was quenched. Based on this

a cysteine/homocysteine(Cys/Hcy) enhanced nanoprobe was constructed using the stronger interaction between Hg

and -SH. Linear relationships were established within the range of 0.1-150 μmol/L for Cys and 0.1-100 μmol/L for Hcy

respectively. The limit detections of Cys and Hcy were 30 nmol/L and 50 nmol/L. In addition

the as-prepared N

P-CDs with strong anti-matrix interference ability could be used for real samples such as tap water and urine. N

P-CDs were applied to sensing Hg

/Cys within living cells favorably based on their ultralow cytotoxicity and outstanding cell permeability

indicating the potential applications in bioimaging and biosensing.

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Related Author

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Le-ping YU

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Related Institution

School of Materials Science and Engineering， Yancheng Institute of Technology

School of Automotive Technology， Wuxi Vocational institute of commerce

State Key Laboratory of Rare Earth Resources Utilization， Changchun Institute of Applied Chemistry， Chinese Academy of Sciences

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Inner Mongolia Key Laboratory of Fine Organic Synthesis, College of Chemistry and Chemical Engineering, Inner Mongolia University

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⁰

Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

DOI：10.37188/CJL.20210389

摘要

Abstract

关键词

Keywords

references

Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots