Fluorescent Detection for Cysteine/Homocysteine Using Hg<sup>2+</sup> Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

Lu LU; Lü XU; Xin ZHANG; Xiao-hong ZHOU; Le-ping YU

doi:10.37188/CJL.20210389

您当前的位置：

首页 >

文章列表页 >

Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

Luminescence Applications and Interdisciplinary Fields | 更新时间：2022-04-07

- Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots
  增强出版
- Chinese Journal of Luminescence Vol. 43, Issue 3, Pages: 440-452(2022)
- 作者机构：
  
  1.无锡商业职业技术学院　汽车技术学院，江苏　无锡　214153
  2.盐城工学院　材料科学与工程学院，江苏　盐城　224002
- 作者简介：
- 基金信息：
  
  Jiangsu Province Natural Science Foundation(BK20191041);Natural Science Foundation of the Jiangsu Higher Education Institutions of China(19KJB480011);Science Foundation of Wuxi Vocational Institute of Commerce school project(KJXJ21604);Production and Education Integration Project of Wuxi Vocational Institute of Commerce school project(XQ202113)
- DOI：10.37188/CJL.20210389
  CLC： O482.31
- Published：2022-03，
  
  Received：14 December 2021，
  
  Revised：29 December 2021，
- 稿件说明：
移动端阅览
LU LU, LÜ XU, XIN ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots. [J]. Chinese journal of luminescence, 2022, 43(3): 440-452.
DOI：

LU LU, LÜ XU, XIN ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots. [J]. Chinese journal of luminescence, 2022, 43(3): 440-452. DOI： 10.37188/CJL.20210389.

摘要

以叶酸和N-(膦酰基甲基)亚氨基二乙酸为原料，制备了具有丰富氮含量(10.0%)和高荧光量子产率(23.64%)的N

P-CDs。由于Hg

与N

P-CDs之间的电子转移作用，N

P-CDs的荧光将被猝灭，基于此构建了半胱氨酸/同型半胱氨酸(Cys/Hcy)增强型纳米探针。利用Hg

和硫醇基团之间更强的相互作用，实现Cys/Hcy的检测。Cys的测定在0.1~150 μmol/L范围内存在线性关系，Hcy的测定在0.1~100 μmol/L范围内存在线性关系，检测限分别为30 nmol/L和50 nmol/L。此外，本文所制备的N

P-CDs具有较强的抗基质干扰能力，可用于自来水和尿液等真实样品检测；因其超低的细胞毒性和出色的细胞渗透性，可用于检测活细胞内的Hg

/Cys，在生物传感方面具有较为广阔的应用前景。

Abstract

A new kind of N

P-CDs with rich nitrogen content(10.0%) and high fluorescence quantum yield(23.64%) was successfully prepared using folic acid and N-(phosphonomethyl)iminodiacetic acid as precursors. Due to the charge transfer between Hg

and N

P-CDs

the fluorescence of N

P-CDs was quenched. Based on this

a cysteine/homocysteine(Cys/Hcy) enhanced nanoprobe was constructed using the stronger interaction between Hg

and -SH. Linear relationships were established within the range of 0.1-150 μmol/L for Cys and 0.1-100 μmol/L for Hcy

respectively. The limit detections of Cys and Hcy were 30 nmol/L and 50 nmol/L. In addition

the as-prepared N

P-CDs with strong anti-matrix interference ability could be used for real samples such as tap water and urine. N

P-CDs were applied to sensing Hg

/Cys within living cells favorably based on their ultralow cytotoxicity and outstanding cell permeability

indicating the potential applications in bioimaging and biosensing.

关键词

NP-CDs荧光探针半胱氨酸检测同型半胱氨酸检测细胞成像

Keywords

N P-CDsfluorescent probescysteine detectionhomocysteine detectioncell image

references

MILLS E L, RYAN D G, PRAG H A, et al. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1 [J]. Nature, 2018, 556(7699):113-117.

SONG Y, LUO Y N, ZHU C Z, et al. Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials [J]. Biosens. Bioelectron., 2016, 76:195-212.

ZHU N S, GUO X L, PANG S R, et al. Mitochondria-immobilized unimolecular fluorescent probe for multiplexing imaging of living cancer cells [J]. Anal. Chem., 2020, 92(16):11103-11110.

XIE X X, PENG Z Q, HUA X Y, et al. Selectively monitoring glutathione in human serum and growth-associated living cells using gold nanoclusters [J]. Biosens. Bioelectron., 2020, 148:111829-1-11.

VAN LEEUWEN K A, NARDIN T, BARKER D, et al. A novel LC-HRMS method reveals cysteinyl and glutathionyl polysulfides in wine [J]. Talanta, 2020, 218:121105-1-9.

IVANOV A V, VIRUS E D, LUZYANIN B P, et al. Capillary electrophoresis coupled with 1,1'-thiocarbonyldiimidazole derivatization for the rapid detection of total homocysteine and cysteine in human plasma [J]. J. Chromatogr. B, 2015, 1004:30-36.

LIU L R, ZHU G B, ZENG W, et al. Silicon quantum dot-coated onto gold nanoparticles as an optical probe for colorimetric and fluorometric determination of cysteine [J]. Microchim. Acta, 2019, 186(2):98-1-9.

CHEN S H, HUANG Z Z, JIA Q. Electrostatically confined in-situ preparation of stable glutathione-capped copper nanoclusters for fluorescence detection of lysozyme [J]. Sens. Actuators B:Chem., 2020, 319:128305-1-8.

LI K B, QU W B, SHEN Q X, et al. 1,8-Naphthalimide-based dual-response fluorescent probe for highly discriminating detection of cys and H2S [J]. Dyes Pigm., 2020, 173:107918-1-6.

WANG Q, ZHOU M, ZHANG L. A dual mode photoelectrochemical sensor for nitrobenzene and L-cysteine based on 3D flower-like Cu2SnS3@SnS2 double interfacial heterojunction photoelectrode [J]. J. Hazard. Mater., 2020, 382:121026-1-10.

WU N T, YU A W, ZHANG L B, et al. Biocompatible nanoplatform based on mussel adhesive chemistry:effective assembly,dual mode sensing,and cellular imaging performance [J]. Adv. Mater. Interfaces, 2019, 6(17):1900732-1-10.

QIAO G X, LIU L, HAO X X, et al. Signal transduction from small particles:sulfur nanodots featuring mercury sensing,cell entry mechanism and in vitro tracking performance [J]. Chem. Eng. J., 2020, 382:122907.

WANG Z X, JIN X, GAO Y F, et al. Fluorometric and colorimetric determination of hypochlorite using carbon nanodots doped with boron and nitrogen [J]. Microchim. Acta, 2019, 186(6):328-1-9.

YANG F, HE X, WANG C X, et al. Controllable and eco-friendly synthesis of P-riched carbon quantum dots and its application for copper (Ⅱ) ion sensing [J]. Appl. Surf. Sci., 2018, 448:589-598.

WANG Z X, YU X H, LI F, et al. Multiplexed ratiometric photoluminescent detection of pyrophosphate using anisotropic boron-doped nitrogen-rich carbon rugby ball-like nanodots [J]. J. Mater. Chem. B, 2018, 6(12):1771-1781.

JIAO Y, GAO Y F, MENG Y Y, et al. One-step synthesis of label-free ratiometric fluorescence carbon dots for the detection of silver ions and glutathione and cellular imaging applications [J]. ACS Appl. Mater. Interfaces, 2019, 11(18):16822-16829.

QU J, ZHANG X, LIU Y F, et al. N,P-co-doped carbon dots as a dual-mode colorimetric/ratiometric fluorescent sensor for formaldehyde and cell imaging via an aminal reaction-induced aggregation process [J]. Microchim. Acta, 2020, 187(6):355-1-10.

ATCHUDAN R, EDISON T N J I, PERUMAL S, et al. Hydrophilic nitrogen-doped carbon dots from biowaste using dwarf banana peel for environmental and biological applications [J]. Fuel, 2020, 275:117821-1-10.

LI Y S, ZHAO L C, LI B L, et al. Evaluating nitrite content changes in some Chinese home cooking with a newely-developed CDs diazotization spectrophotometry [J]. Food Chem., 2020, 330:127151.

LI M, LI X N, JIANG M Y, et al. Engineering a ratiometric fluorescent sensor membrane containing carbon dots for efficient fluoride detection and removal [J]. Chem. Eng. J., 2020, 399:125741.

SUN D, LIU T T, WANG C F, et al. Hydrothermal synthesis of fluorescent carbon dots from gardenia fruit for sensitive on-off-on detection of Hg2+ and cysteine [J]. Spectrochim. Acta, Part A, 2020, 240:118598-1-8.

ZHANG C, LIU M L, LI T T, et al. One-pot hydrothermal synthesis of dual-emission fluorescent carbon dots for hypochlorous acid detection [J]. Dyes Pigm., 2020, 180:108507-1-7.

LI G J, GUAN Y H, YE F Y, et al. Cyanine-based fluorescent indicator for mercury ion and bioimaging application in living cells [J]. Spectrochim. Acta Part A, 2020, 239:118465-1-6.

KUMAR A, KUMAR S, CHAE P S. A novel anthrapyridone diamine-based probe for selective and distinctive Cu2+ and Hg2+ sensing in aqueous solution; utility as molecular logic gates [J]. Dyes Pigm., 2020, 181:108522-1-14.

PAJEWSKA-SZMYT M, BUSZEWSKI B, GADZAŁA-KOPCIUCH R. Carbon dots as rapid assays for detection of mercury(Ⅱ) ions based on turn-off mode and breast milk [J]. Spectrochim. Acta Part A, 2020, 236:118320-1-8.

SUN Y C, WANG L Z, ZHOU J H, et al. A new phenothiazine-based fluorescence sensor for imaging Hg2+ in living cells [J]. Appl. Organomet. Chem., 2020, 34(11):e5945-1-11.

YE Z G, ZHANG Y H, LI G X, et al. Fluorescent determination of mercury(Ⅱ) by green carbon quantum dots synthesized from eggshell membrane [J]. Anal. Lett., 2020, 53(18):2841-2853.

PAN Z X, XU Z X, CHEN J, et al. Coumarin thiourea-based fluorescent turn-on Hg2+ probe that can be utilized in a broad pH range http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=25657629&type=http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=25657635&type=http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=25657632&type=11 [J]. J. Fluoresc., 2020, 30(3):505-514.

GHOSH T, CHATTERJEE S, BHAYANI K, et al. A natural cyanobacterial protein C-phycoerythrin as an Hg2+ selective fluorescent probe in aqueous systems [J]. New J. Chem., 2020, 44(16):6601-6609.

CHU H T, YAO D, CHEN J Q, et al. Double-emission ratiometric fluorescent sensors composed of rare-earth-doped ZnS quantum dots for Hg2+ detection [J]. ACS Omega, 2020, 5(16):9558-9565.

REN H X, HUO F J, ZHANG Y B, et al. An NIR ESIPT-based fluorescent probe with large stokes shift for specific detection of Cys and its bioimaging in cells and mice [J]. Sens. Actuators B:Chem., 2020, 319:128248-1-8.

ZHOU P C, SHE M Y, LIU P, et al. Measuring the distribution and concentration of cysteine by fluorescent sensor for the visual study of paracetamol-induced pro-sarcopenic effect [J]. Sens. Actuators B: Chem., 2020, 318:128258.

ZHONG K L, ZHOU S Y, YAN X M, et al. A novel D-π-A type NBD-based fluorescent probe for ultrafast and distinguishable detection of Hcy/Cys and its bioimaging application [J]. J. Lumin., 2020, 224:117330.

JIANG X Q, HUANG J B, CHEN T Y, et al. Synthesis of hemicellulose/deep eutectic solvent based carbon quantum dots for ultrasensitive detection of Ag+ and L-cysteine with “off-on” pattern [J]. Int. J. Biol. Macromol., 2020, 153:412-420.

YUAN C L, QIN X, XU Y J, et al. Carbon quantum dots originated from chicken blood as peroxidase mimics for colorimetric detection of biothiols [J]. J. Photochem. Photobiol. A: Chem., 2020, 396:112529.

LIU C Y, MIAO Y Q, ZHANG X J, et al. Colorimetric determination of cysteine by a paper-based assay system using aspartic acid modified gold nanoparticles [J]. Microchim. Acta, 2020, 187(6):362-1-8.

SUN Z B, HUA Y, GAO M J, et al. Highly selective fluorescent 4-(4-(diethylamino)-2-hydroxystyryl)-1-methylpyridine iodide and nitrobenzofurazan based probe for cysteine with application in living cells [J]. Anal. Lett., 2021, 54(3):417-429.

鲁诗言, 于淑娟, 陈国全, 等. 氮、磷掺杂碳点的合成及在Pd2+传感中的应用 [J]. 发光学报, 2021, 42(1):53-60.

LU S Y, YU S J, CHEN G Q, et al. Synthesis of nitrogen and phosphorus doped carbon dots and their application in Pd2+ sensing [J]. Chin. J. Lumin., 2021, 42(1):53-60. (in Chinese)

HUANG S, YANG E L, YAO J D, et al. Red emission nitrogen,boron,sulfur co-doped carbon dots for “on-off-on” fluorescent mode detection of Ag+ ions and L-cysteine in complex biological fluids and living cells [J]. Anal. Chim. Acta, 2018, 1035:192-202.

FARHADIAN S, SHAREGHI B, SABOURY A A, et al. Molecular aspects of the interaction of spermidine and α-chymotrypsin [J]. Int. J. Biol. Macromol., 2016, 92:523-532.

王宁, 王新旭, 杨海芬, 等. 基于氮磷共掺杂碳点的荧光探针检测汞离子 [J]. 化学研究与应用, 2019, 31(3):440-448.

WANG N, WANG X X, YANG H F, et al. A fluorescent probe for determination of mercury ion based on phosphorus and nitrogen co-doped carbon dot [J]. Chem. Res. Appl., 2019, 31(3):440-448. (in Chinese)

Views

404

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Progress in Research and Application of New Optical Functional Rare Earth Complexes

Research Progress of Fluorescent Probes for Monoamine Oxidases

Progress on Aggregation-induced Emission Probes for Mitochondria Target and Cancer Cell Identification

Near-infrared Fluorescent Probe Based on Methylene Blue for Specific Detection of Hypochlorous Acid

Related Author

Lu LU

Le-ping YU

Xin ZHANG

Xiao-hong ZHOU

Lyu XU

DENG Rui-ping

WANG Hao

WANG Hong-yu

Related Institution

School of Materials Science and Engineering， Yancheng Institute of Technology

School of Automotive Technology， Wuxi Vocational institute of commerce

State Key Laboratory of Rare Earth Resources Utilization， Changchun Institute of Applied Chemistry， Chinese Academy of Sciences

School of Materials Science and Engineering， Jilin Jianzhu University

Inner Mongolia Key Laboratory of Fine Organic Synthesis, College of Chemistry and Chemical Engineering, Inner Mongolia University

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176862 Email：fgxbt@126.com
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

DOI：10.37188/CJL.20210389

摘要

Abstract

关键词

Keywords

references

Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots