Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation

Xue-hua SUN; Yu QIANG; Du-ting HAO; Rui TIAN

doi:10.37188/CJL.20210271

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Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation

Luminescence Applications and Interdisciplinary Fields | 更新时间：2021-12-17

- Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation
  增强出版
- Chinese Journal of Luminescence Vol. 42, Issue 12, Pages: 1951-1960(2021)
- 作者机构：
  
  延安大学化学与化工学院　延安市绿色合成材料与化学安全检测重点实验室，陕西　延安　716000
- 作者简介：
- 基金信息：
  
  the Natural Science Special Project of Shaanxi Province(2020JQ-789);Key Scientific Research Projects of Shaanxi Provincial Department of Education(20JS156)
- DOI：10.37188/CJL.20210271
  CLC： O657.31
- Published：2021-12，
  
  Received：26 August 2021，
  
  Revised：15 September 2021，
扫描看全文
Xue-hua SUN, Yu QIANG, Du-ting HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation. [J]. Chinese Journal of Luminescence 42(12):1951-1960(2021)
DOI：

Xue-hua SUN, Yu QIANG, Du-ting HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation. [J]. Chinese Journal of Luminescence 42(12):1951-1960(2021) DOI： 10.37188/CJL.20210271.

摘要

通过一步水热法合成了荧光量子产率高达34%的锌掺杂碳量子点(Zinc doped carbon quantum dots

Zn-CQDs)，并对Zn-CQDs的结构性能进行分析。基于Cu

与H

产生羟基自由基可有效猝灭Zn-CQDs，构建了Zn-CQDs-Cu

荧光探针检测过氧化氢和胆固醇浓度。实验发现，在pH=7.60的N-2-羟乙基哌嗪-N'-2-乙磺酸(HEPES)缓冲溶液中，于50 ℃孵化40 min时，体系的(

-F

)

与H

浓度在1.0×10

-5

~1.0×10

-6

mol/L范围内，与胆固醇浓度在3.0×10

-5

~9.0×10

-7

mol/L范围内分别呈良好的线性关系，检出限分别为7.2×10

-7

mol/L和6.8×10

-7

mol/L。该方法用于水样中H

和牛奶中胆固醇含量的测定，回收率分别可达98.55%~105.4%和98.00%~103.0%，结果满意。该方法也为检测H

生成反应的其他代谢物提供了有效的检测思路。

Abstract

Zinc doped carbon quantum dots (Zn-CQDs) with fluorescence quantum yield up to 34% were synthesized by one step hydrothermal method

and the structural properties of Zn-CQDs were analyzed. Based on the fact that hydrogen peroxide produced by Cu

and H

can effectively quench Zn-CQDs

a Zn-CQDs-Cu

fluorescent probe was constructed to detect hydrogen peroxide and indirectly detect cholesterol concentration. The effects of buffer type

reaction time and temperature on the determination of H

and cholesterol were investigated. The results showed that (

-F

)

of the system has good linear relationship with the concentration of H

when incubated at 50 ℃ for 40 min in HEPES buffer solution with pH=7.06. The linear range was 1.0×10

-5

~1.0×10

-6

mol/L. The detection limit was 7.2×10

-7

mol/L. The (

-F

)

of the system also has good linear relationship with concentration of cholesterol. The linear range was 3.0×10

-5

-9.0×10

-7

mol/L

and the detection limit was 6.8×10

-7

mol/L. The method was used for the determination of H

in water samples and cholesterol content in milk

the detection recovery rate can reach 98.55%-105.4% and 98.00%-103.0% respectively

and the result was satisfactory. It can also provide effective detection ideas for the detection of other metabolites in the H

reaction.

关键词

锌掺杂碳量子点过氧化氢胆固醇荧光猝灭

Keywords

zinc doped carbon quantum dotshydrogen peroxidecholesterolfluorescence quenching

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Related Author

Xue-Hua SUN

Yu QIANG

Du-Ting HAO

Rui TIAN

Jing CHEN

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Yu-hao HUANG

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Related Institution

Yan'an Key Laboratory of Green Synthetic Materials and Chemical safety testing ，College of Chemistry and Chemical Engineering ， Yan'an University

School of Optics and Electronic Information, Huazhong University of Science and Technology

State Key Laboratory on Integrated Optoelectronics, Jilin University

School of Chemistry and Chemical Engineering, Yangtze Normal University

School of Environment and Civil Engineering, Dongguan University of Technology

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