基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测

冯雨晴; 李奚

doi:10.3788/fgxb20194010.1215

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基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测

材料合成及性能 | 更新时间：2020-08-12

- 基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测
- Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells
- 发光学报 2019年40卷第10期页码：1215-1219
- 作者机构：
  
  长春工业大学化学与生命科学学院,吉林长春,130012
- 作者简介：
  
  [ "冯雨晴(1992-),女,河南鹤壁人,硕士研究生,2015年于长春工业大学获得学士学位,主要从事纳米材料生物学应用方面的研究。E-mail:806644833@qq.com" ]
  [ "李奚(1979-),女,江苏常州人,博士,教授,博士研究生导师,2007年于中国科学院长春应用化学研究所获得博士学位,主要从事纳米化学生物学的研究。E-mail:xli@ccut.edu.cn" ]
- 基金信息：
  
  吉林省教育厅"十三五"科学技术项目（JJKH20170543KJ）资助
- DOI：10.3788/fgxb20194010.1215
  中图分类号： Q599
- 收稿日期：2019-05-27，
  
  修回日期：2019-06-30，
  
  纸质出版日期：2019-10-05
- 稿件说明：
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冯雨晴, 李奚,. 基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测[J]. 发光学报, 2019,40(10): 1215-1219

FENG Yu-qing, LI Xi,. Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells[J]. Chinese Journal of Luminescence, 2019,40(10): 1215-1219
冯雨晴, 李奚,. 基于SERS技术的靶向修饰金纳米探针用于Hela细胞的检测[J]. 发光学报, 2019,40(10): 1215-1219 DOI： 10.3788/fgxb20194010.1215.

FENG Yu-qing, LI Xi,. Surface Enhanced Raman Scattering Probes Based onTargeted Gold Nanorods for Detecting Hela Cells[J]. Chinese Journal of Luminescence, 2019,40(10): 1215-1219 DOI： 10.3788/fgxb20194010.1215.

摘要

表皮生长因子受体（EGFR）是一种肿瘤表面标记性蛋白。本文报道了基于anti-EGFR功能化金纳米棒探针AuNRs probes的表面增强拉曼散射（SERS），用于EGFR阳性肿瘤细胞的检测。通过AuNRs probes上anti-EGFR特异性结合到EGFR阳性癌细胞上，可使修饰于金纳米棒表面的拉曼活性染料4-巯基苯甲酸（4-MBA）位于1 100 cm

-1

和1 600 cm

-1

的特征峰强度得到信号增强。该SERS探针由于具有生物兼容性好、细胞拉曼信号稳定、特异性高等优点而具有巨大的临床应用前景。

Abstract

Epidermal growth factor receptor(EGFR)is overexpressed on tumor surface. A surface enhanced Raman scattering(SERS) probe based on epidermal growth factor receptor antibody(anti-EGFR) conjugated Au nanorods(AuNRs probes) was used for EGFR positive cancer cell detection. When AuNRs probes were specific bound to EGFR positive cell

SERS signals from Raman active molecule 4-mercaptobenzoic acid(4-MBA) modified on AuNRs were two to three times more than those cells incubated with 4-MBA labeled AuNRs(AuNRs-MBA). This kind of SERS probe has great potential in the diagnosis of EGFR positive tumor due to its specific targeting

high SERS sensitivity and biocompatibility.

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references

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