纳米银粒子增强铕（Ⅲ）配合物荧光对鸭蛋蛋清中强力霉素残留量的检测

洪茜; 赵进辉; 袁海超; 彭义杰; 李耀; 刘木华

doi:10.3788/fgxb20143510.1252

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纳米银粒子增强铕（Ⅲ）配合物荧光对鸭蛋蛋清中强力霉素残留量的检测

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 纳米银粒子增强铕（Ⅲ）配合物荧光对鸭蛋蛋清中强力霉素残留量的检测
- Silver Nanoparticles Enhanced Fluorescence of Europium (Ⅲ) Complex for Determination of Doxycycline Content in Duck Egg White
- 发光学报 2014年35卷第10期页码：1252-1257
- 作者机构：
  
  江西农业大学工学院生物光电及应用重点实验室,江西南昌,330045
- 作者简介：
- 基金信息：
  
  国家自然科学基金（31101295）;江西省科技厅对外科技合作计划（20132BDH80005）;江西省科技厅科技支撑（2012BBG70058）;江西省教育厅科技计划（GJJ12244）资助项目
- DOI：10.3788/fgxb20143510.1252
  中图分类号： O657.31
- 收稿日期：2014-07-17，
  
  修回日期：2014-08-02，
  
  纸质出版日期：2014-10-03
- 稿件说明：
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洪茜, 赵进辉, 袁海超等. 纳米银粒子增强铕（Ⅲ）配合物荧光对鸭蛋蛋清中强力霉素残留量的检测[J]. 发光学报, 2014,35(10): 1252-1257

HONG Qian, ZHAO Jin-hui, YUAN Hai-chao etc. Silver Nanoparticles Enhanced Fluorescence of Europium (Ⅲ) Complex for Determination of Doxycycline Content in Duck Egg White[J]. Chinese Journal of Luminescence, 2014,35(10): 1252-1257
洪茜, 赵进辉, 袁海超等. 纳米银粒子增强铕（Ⅲ）配合物荧光对鸭蛋蛋清中强力霉素残留量的检测[J]. 发光学报, 2014,35(10): 1252-1257 DOI： 10.3788/fgxb20143510.1252.

HONG Qian, ZHAO Jin-hui, YUAN Hai-chao etc. Silver Nanoparticles Enhanced Fluorescence of Europium (Ⅲ) Complex for Determination of Doxycycline Content in Duck Egg White[J]. Chinese Journal of Luminescence, 2014,35(10): 1252-1257 DOI： 10.3788/fgxb20143510.1252.

摘要

强力霉素能与铕（Ⅲ）相互作用生成EuDC配合物并发射出铕（Ⅲ）的荧光特征峰，且该荧光峰能够通过纳米银粒子的金属增强荧光效应得到进一步增强。本文将这一方法应用于鸭蛋蛋清中强力霉素残留量的检测。首先，利用三维荧光光谱法确定了检测鸭蛋蛋清中强力霉素残留量的最佳激发波长和最佳发射波长分别为390 nm和617 nm。然后，在最佳激发波长下，通过单因素实验确定了实验的最佳条件。最后，通过对浓度范围为0.5~30 mg/L的样本进行分析，发现鸭蛋蛋清中强力霉素的浓度与荧光强度呈现良好的线性关系，并得到检测限为0.5 mg/L。研究结果表明，通过纳米银粒子增强铕（Ⅲ）的荧光可以实现鸭蛋蛋清中强力霉素的快速检测。

Abstract

Doxycycline can coordinate with europium (Ⅲ) to form EuDC complex which emits characteristic fluorescence peak of europium (Ⅲ). The fluorescence of EuDC complex can be further enhanced by silver nanoparticles through the metal-enhanced fluorescence effect. This method was applied to determine the doxycycline content in duck egg white in this essay. Firstly

390 nm and 617 nm were selected as the optimum excitation wavelength and emission wavelength by using three-dimensional fluorescence spectrometry. Secondly

the optimum experiment conditions were determined by single factor experiment under the optimum excitation wavelength. Finally

the samples of concentration range of 0.5~30 mg/L were analyzed

and the fluorescence intensity displayed a good linear relationship to the doxycycline concentrations in duck egg white with a detection limit of 0.5 mg/L. The experiment results indicate that the method of silver nanoparticles enhanced fluorescence of europium (Ⅲ) can achieve the rapid determination of doxycycline content in duck egg white.

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references

Shen L M, Yao J L, Gu R A. Electrochemical synthesis and spectroscopic characterization of gold nanoparticles[J]. Spectrosc. Spect. Anal.(光谱学与光谱分析), 2005, 25(12):1998-2001 (in Chinese).

Deng S M, Zou Y J, Wang X D, et al. Quenching effect of silver nanoparticles on fluorescence of norfloxacin[J]. Chin. J. Spectrosc. Lab.(光谱实验室), 2012, 29(1):16-20 (in Chinese).

Liu Z, Mao X F, Wang H Y, et al. Quenching effect of silver nanoparticles on the fluorescence of eosine[J]. Chem. Reag.(化学试剂), 2009, 31(6):463-465 (in Chinese).

Hei T T. Organophosphate Biosensor Based on Fluorescence Enhancement with Metal Nanoparticles[D]. Xi'an: Shaanxi Normal University, 2011 (in Chinese).

Li H X, Huang H G. Fluorescence property of complexes of europium and samarium with tetracycline antibiotics and their application in fluorimetry[J]. Chin. J. Anal. Chem.(分析化学), 1995, 23(9):1036-1039 (in Chinese).

Yin D G, Zhang L, Liu B H, et al. Preparation and characterization of a novel type of dual-lanthanide-complexs doped Ag@silica fluorescent nanoparticles[J]. Acta Chim. Sinica (化学学报), 2010, 68(19):1965-1970 (in Chinese).

FuY, ZhangJ, Lakowicz J R. Metal-enhanced fluorescence of single green fluorescent protein(GFP)[J]. Biochem. Bioph. Res. Co., 2008, 376(4):712-717.

McWhinney B C, Wallis S C, Hillister T, et al. Analysis of 12 beta-lactam antibiotics in human plasma by HPLC with ultraviolet detection[J]. J. Chromatogr. B, 2010, 878(22):2039-2043.

Liu X Q, Feng Z, Yao L, et al. Analysis of tetracycline and neomycin antibiotic residues in eggs using throughout microbial assay[J]. Mod. Food Sci. Technol.(现代食品科技), 2011, 27(4):465-467 (in Chinese).

Chen T B, Deng W H, Lu W H, et al. Detection of residual antibiotics in honey with capillary electrophoresis[J]. Chin. J. Chromatogr.(色谱), 2011, 19(1):91-93 (in Chinese).

Bao L Y, Gu J L, Che B Q. Quantitative analysis of four antibiotics by high performance capillary electrophoresis[J]. J. Beijing Inst. Technol.(北京理工大学学报), 2001, 21(2):270-272 (in Chinese).

Li N B, Duan J P, Chen H Q, et al. Flow injection analysis of trace amounts of doxycycline with inhibited chemiluminescence detection[J]. Spectrosc. Spect. Anal.(光谱学与光谱分析), 2004, 24(1):15-17 (in Chinese).

Xiao H B, Zhao J H, Yuan H C, et al. Determination of tetracycline content in pork by synchronous fluorescence spectroscopy with CARS method[J]. Opt. Precision Eng.(光学精密工程), 2013, 21(10):2513-2519 (in Chinese).

Zhang Y P. Simultaneous Detection of Sulfamethoxazole and Danofloxacin Resdues in Milk by Fluorescence Spectroscopy[D]. Wuxi: Jiangnan University, 2013 (in Chinese).

Zhang L W, Luo L, Jiang C Q, et al. Spectrofluorimetric determination of trace doxycycline with diethyl-O--cyclodextrin-doxycycline-Eu3+ system[J]. Chin. J. Anal. Chem.(分析化学), 2008, 36(11):1547-1550 (in Chinese).

Tan H L, Chen Y. Silver nanoparticle enhanced fluorescence of europium(Ⅲ) for detection of tetracycline in milk[J]. Sens. Actuat. B, 2012, 173:262-267.

Qu L L, Li D W, Zhai W L, et al. Rapid on-site detection of aromatic amines in water by surface-enhanced raman spectrometry[J]. Environ. Chem.(环境化学), 2011, 30(8):1486-1492 (in Chinese).

Hou F H, Zhang H X, Chu Q K, et al. Fluorescence enhancement of Ag@SiO2 nano composite to carboxylate europium complexes[J]. Chin. J. Lumin.(发光学报), 2012, 33(11):1181-1186 (in Chinese).

Xie Y, Wang T, Wang H B. Luminescence properties of Y(VP)O4:Eu3+ phosphor doped with alkali earths and rare earths[J]. Chin. J. Liq. Cryst. Disp.(液晶与显示), 2011, 26(5):587-591 (in Chinese).

Chen Q. Determination of Tetracycline Antibiotics Residues in Animal-derived Food by HPLC[D]. Zhengzhou: Henan Agricultural University, 2009 (in Chinese).

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