基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定

季仁东; 赵志敏; 张吉华; 陈梦岚; 朱星玥

doi:10.3788/fgxb20143508.1014

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基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定
- Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe
- 发光学报 2014年35卷第8期页码：1014-1017
- 作者机构：
  
  1. 南京航空航天大学理学院,江苏南京,210016
  2. 淮阴工学院电子与电气工程学院,江苏淮安,223003
- 作者简介：
- 基金信息：
  
  国家自然科学基金（10722043）();教育部博士点基金（20093218110024）();江苏省普通高校研究生科研创新计划（CXLX12_0143）();南京航空
- DOI：10.3788/fgxb20143508.1014
  中图分类号： O433.4
- 收稿日期：2014-05-13，
  
  修回日期：2014-06-03，
  
  纸质出版日期：2014-08-03
- 稿件说明：
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季仁东, 赵志敏, 张吉华等. 基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定[J]. 发光学报, 2014,35(8): 1014-1017

JI Ren-dong, ZHAO Zhi-min, ZHANG Ji-hua etc. Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2014,35(8): 1014-1017
季仁东, 赵志敏, 张吉华等. 基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定[J]. 发光学报, 2014,35(8): 1014-1017 DOI： 10.3788/fgxb20143508.1014.

JI Ren-dong, ZHAO Zhi-min, ZHANG Ji-hua etc. Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2014,35(8): 1014-1017 DOI： 10.3788/fgxb20143508.1014.

摘要

对牛血清白蛋白（BSA）与盐酸特拉唑嗪（THD）混合体系进行了荧光发射光谱和同步光谱实验研究。BSA溶液在340 nm处有明显荧光特征峰，当加入THD后导致牛血清白蛋白发生内源荧光猝灭。据此建立了以BSA为探针的THD含量测定方法，通过该方法所得的模型函数相关系数都高于0.99。对所得模型函数进行了实验验证，其中发射光谱模型函数回收率为96.04%~103.16%，同步光谱模型函数回收率为100.41%~ 105.58%，相对标准偏差分别为3.70%和2.42%。荧光发射光谱和同步光谱方法的检出限分别为0.6410

-8

mol/L和0.6610

-8

mol/L，定量限分别为0.2110

-7

mol/L和0.2210

-7

mol/L。

Abstract

The fluorescence emission and synchronous spectra of the bovine serum albumin (BSA) and terazosin hydrochloride (THD) mixed system were studied. The most intensive characteristic peak (340 nm) was found in the spectrum of BSA solution

and the addition of THD resulted in the fluorescence quenching of BSA. Based on this phenomenon

the determination method for THD was established

in which BSA was used as the fluorescent probe. The correlation coefficients of the model functions are all more than 0.99. The recoveries of emission and synchronous spectra are 96.04%~103.16% and 100.41%~105.58%

the relative standard deviations (RSD) are 3.70% and 2.42%

the limits of detection are 0.6410

-8

mol/L and 0.6610

-8

mol/L

and the limits of quantification are 0.2110

-7

mol/L and 0.2210

-7

mol/L

respectively.

关键词

Keywords

references

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Ji R D, Zhao Z M, Zhang L, et al. Study on experiment of fluorescence spectra detection of pesticide residue about imidacloprid in apple juices [J]. Spectrosc. Spect. Anal.(光谱学与光谱分析), 2013, 33(3):668-671 (in Chinese).

Poulli K I, Mousdis G A, Georgiou C A. Synchronous fluorescence spectroscopy for quantitative determination of virgin olive oil adulteration with sunflower oil [J]. Anal. Bioanal. Chem., 2006, 386(5):1571-1575.

Wang T H, Zhao Z M, Wei B Z, et al. Spectroscopic investigations on the binding of dibazol to bovine serum albumin [J]. J. Mol. Struct., 2010, 970(1-3):128-133.

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