基于生物延迟发光评价玉米萌发期抗旱性的方法

高宇; 习岗; 刘锴; 杨运经

doi:10.3788/fgxb20143502.0243

您当前的位置：

首页 >

文章列表页 >

基于生物延迟发光评价玉米萌发期抗旱性的方法

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 基于生物延迟发光评价玉米萌发期抗旱性的方法
- Evaluation Method of Maize Drought Resistance During Germination Based on Delayed Luminescence
- 发光学报 2014年35卷第2期页码：243-250
- 作者机构：
  
  1. 西安理工大学应用物理系, 陕西西安 710048
  2. 西北农林科技大学应用物理系, 陕西杨凌 712100
- 作者简介：
- 基金信息：
  
  国家自然科学基金（50977079，51277151）资助项目()
- DOI：10.3788/fgxb20143502.0243
  中图分类号： Q632
- 收稿日期：2013-09-11，
  
  修回日期：2013-10-20，
  
  纸质出版日期：2014-02-03
- 稿件说明：
移动端阅览
高宇, 习岗, 刘锴等. 基于生物延迟发光评价玉米萌发期抗旱性的方法[J]. 发光学报, 2014,35(2): 243-250

GAO Yu, XI Gang, LIU Kai etc. Evaluation Method of Maize Drought Resistance During Germination Based on Delayed Luminescence[J]. Chinese Journal of Luminescence, 2014,35(2): 243-250
高宇, 习岗, 刘锴等. 基于生物延迟发光评价玉米萌发期抗旱性的方法[J]. 发光学报, 2014,35(2): 243-250 DOI： 10.3788/fgxb20143502.0243.

GAO Yu, XI Gang, LIU Kai etc. Evaluation Method of Maize Drought Resistance During Germination Based on Delayed Luminescence[J]. Chinese Journal of Luminescence, 2014,35(2): 243-250 DOI： 10.3788/fgxb20143502.0243.

摘要

玉米种子萌发期抗旱性评价是节水农业研究中的难点和热点问题之一，生物延迟发光分析技术的应用有可能解决这一问题。采用生物延迟发光评价方法研究了玉米种子萌发期的抗旱性能力，探讨了在渗透势-0.1 MPa和-0.3 MPa的PEG-6000溶液中萌发的玉米品种万瑞168号和堰单8号延迟发光的变化规律。结果表明，万瑞168号和堰单8号玉米品种的延迟发光积分强度都随着萌发进程逐渐升高，-0.1 MPa和-0.3 MPa的PEG-6000溶液形成的干旱胁迫对两个玉米品种延迟发光积分强度的升高有不同的抑制作用，胁迫强度越大，两个品种延迟发光积分强度的差异就越大。研究还发现，在干旱胁迫下萌发的万瑞168号和堰单8号玉米延迟发光相对变化率

与种子萌发抗旱指数和储藏物质转运率的变化是一致的，依据干旱胁迫下种子萌发过程中延迟发光积分强度相对变化率

的大小可以评价玉米种子萌发期抗旱性的强弱。

Abstract

Evaluation of drought resistance during germination of maize is one of the basic problems that have not been solved in modern water-saving agriculture

while biological delayed luminescence technology may solve this problem. In order to explore the method of using delayed luminescence to evaluate drought resistance in maize germinating stage

the delayed luminescence of two maize varieties Wanrui No.168 and Yandan No.8 germinated in PEG-6000 solution with osmotic potential of-0.1 MPa and-0.3 MPa was measured. The results show that the integrated intensity of delayed luminescence of Wanrui No. 168 and Yandan No. 8 increase gradually with the germination process. Drought stress with osmotic potential of-0.1 MPa and-0.3 MPa inhibits the increase in integrated intensity of delayed luminescence of Wanrui No.168 and Yandan No.8

and the stronger the stress

the greater the difference of integrated intensity of delayed luminescence between two maize varieties. The relative change rate of delayed luminescence is defined as

. The study found that the change of

of Wanrui No. 168 and Yandan No. 8 was the same as the change of seed germination drought index and storage material transport rate under drought stress. It indicated that the size of

during germination under drought stress could be used to evaluate drought resistance of maize during seed germination.

关键词

Keywords

references

Wu P T, Feng H, Niu W Q, et al. Technical trend and R&D focus of modern water-saving agriculture [J]. Eng. Sci.(中国工程科学), 2007, 9(2):12-18 (in Chinese). [2] Chen Z B. Research advance and development trends of water use efficiency in plant biology [J]. Sci. Agr. Sinica (中国农业科学), 2007, 40(7):1456-1462 (in Chinese). [3] Sun C X, Wu Z J, Zhang Z P, et al. System analysis of drought resistance identification parameters in maize [J]. System Sciences and Comprehensive Studies in Agriculture (农业系统科学与综合研究), 2004, 20(1):43-47 (in Chinese). [4] Sun J W, Ji T H, Yang Z G, et al. Study on identification of the drought resistant in maize seedling emergence stage [J]. Chin. Agr. Sci. Bull.(中国农学通报), 2009, 25(3):104-107 (in Chinese). [5] Zhou W, Hou J H, Gao Z J. Selection of the drought resistance indexes in maize seedling emergence stage [J]. J.Maize Sci.(玉米科学), 2008, 16(5):66-69 (in Chinese). [6] Zhao M L. Studies on identification indices for drought resistance in different growing periods of corn [J]. Chin. Agr. Sci. Bull.(中国农学通报), 2009, 25(12):66-68 (in Chinese). [7] Leng Y F, Zhang B, Zhao J R, et al. Identification of drought resistance of transgenic maize during seed germination stage [J]. Agr. Res. Arid Areas (干旱地区农业研究), 2013, 31(1):177-182 (in Chinese). [8] Tang P S, Xiao J P. Certain modes of control of life process operation by respiratory metabolism in plant cells [J]. J. Integr. Plant Biol., 1991, 33(10):729-737. [9] Shan L. Development trend of dryland farming technologies [J]. Sci. Agr. Sinica (中国农业科学), 2002, 35(7):848-855 (in Chinese). [10] Xi G, Zhang Z Y. Using physics to study plant resistance to adversity [J]. Phys.(物理), 1997, 26(3):162-166 (in Chinese). [11] Zhang X H, Li F J, Shen L, et al. Application of ultraweak luminescence technique in research of plant stress physiology [J]. Plant Physiol. Commun.(植物生理学通讯), 2009, 45(9):931-935 (in Chinese). [12] Burke J J. Evaluation of source leaf responses to water-deficit stresses in cotton using a novel stress bioassay [J]. Plant Physiol., 2007, 143(1):108-121. [13] Burke J J, Franks C D, Burow G, et al. Selection system for the stay-green drought tolerance trait in sorghum germplasm [J]. Agronomy J., 2010, 102(4):1118-1122. [14] Mansfield J W. Biophoton distress flares signal the onset of the hypersensitive reaction [J]. Trends in Plant Sci., 2005, 10(7):307-309. [15] Yoon Y Z, Kim J, Lee B C. Changes in ultraweak photon emission and heart rate variability of epinephrine-injected rats [J]. General Physiol. Biophys., 2005, 24(2):147-159. [16] Yoshinaga N, Kato K, Kageyama C. Ultraweak photon emission from herbivory-injured maize plants [J]. Naturwissenschaften, 2006, 93(1):38-41. [17] Hidehiro I, Toshiyuki I, Wang G X, et al. Spontaneous ultraweak photon emission from rice (Oryza sativa L.) and paddy weeds treated with a sulfonylurea herbicide [J]. Pesticide Biochem. Physiol., 2007, 89(2):158-162. [18] Yu Y, Popp F A, Sibylle S. Further analysis of delayed luminescence of plants [J]. Photochem. Photobiol. B: Biol., 2005, 78(3):235-244. [19] Gu Q. Biophotonics [M]. 2nd ed. Beijing: Science Press, 2012:25-33 (in Chinese). [20] Xi G, Yang Y J, Li S H, et al. Double-exponential model of ultraweak photon emission of soybean callus and its significance [J]. Chin. J. Lumin.(发光学报), 2011, 32(1):87-93 (in Chinese). [21] Wang H M. Effect of salt stress with different time on delayed luminescence from leaves [J]. Chin. J. Lumin.(发光学报), 2009, 30(4):545-548 (in Chinese). [22] Li Y, Xu W H. Detection of stress physiology of plant using F730/F685 ratio of delayed fluorescence spectrum [J]. Chin. J. Lumin.(发光学报), 2011, 32(7):740-747 (in Chinese). [23] Xi G, Li S H, Zhang Y. Analysis of delayed luminescence dynamic processes of spinach leaves stressed by H2O2 [J]. Chin. J. Lumin.(发光学报), 2010, 31(6):952-956 (in Chinese). [24] Xi G, Zhang X H, Liu K, et al. Evaluation method of UV-B radiation injury on plant cell based on photonic technology [J]. Chin. J. Lumin.(发光学报), 2011, 32(4):398-403 (in Chinese). [25] Michel B E, Kaufmann M R. The osmotic potential of polyethylene glycol 6000 [J]. Plant Physiol., 1973, 51(5):914-916. [26] Bouslama M, Schapaugh W T. Stress tolerance in soybeans.Ⅰ .Evaluation of three screening techniques for heat and drought tolerance [J]. Crop Sci., 1984, 24(5):933- 937. [27] Li S H, Xi G, Fan L L, et al. Changes of ultra-weak photon emission of wheat seed during germination and its significance under osmotic stress [J]. Acta Photonica Sinica (光子学报), 2011, 40(2):282-288 (in Chinese). [28] Xi G, Liu K, Zhang X H, et al. Dynamic analysis of ultra-weak photon radiation of soybean callus induced by UV-B radiation [J]. Acta Photonica Sinica (光子学报), 2010, 39(8):1449-1454 (in Chinese).

浏览量

136

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于高光谱图像信息融合的红提糖度无损检测