丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究

李小芳; 冯小强; 杨声; 王霞

doi:10.3788/fgxb20133412.1662

您当前的位置：

首页 >

文章列表页 >

丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究
- Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods
- 发光学报 2013年34卷第12期页码：1662-1666
- 作者机构：
  
  1. 天水师范学院生命科学与化学学院,甘肃天水,74100
  2. 定西师范高等专科学校,甘肃定西,743000
- 作者简介：
- 基金信息：
  
  天水师范学院“青蓝”人才工程资助项目
- DOI：10.3788/fgxb20133412.1662
  中图分类号： O614.121
- 收稿日期：2013-07-26，
  
  修回日期：2013-09-25，
  
  纸质出版日期：2013-12-10
- 稿件说明：
移动端阅览
李小芳, 冯小强, 杨声, 王霞. 丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究[J]. 发光学报, 2013,34(12): 1662-1666

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, WANG Xia. Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods[J]. Chinese Journal of Luminescence, 2013,34(12): 1662-1666
李小芳, 冯小强, 杨声, 王霞. 丁二酰化壳寡糖稀土配合物与鲱鱼精DNA相互作用的电化学及光谱学研究[J]. 发光学报, 2013,34(12): 1662-1666 DOI： 10.3788/fgxb20133412.1662.

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, WANG Xia. Interaction of Succinic-oligochitosan Rare Earth Complexes with Herring Sperm DNA by Electrochemical and Spectral Methods[J]. Chinese Journal of Luminescence, 2013,34(12): 1662-1666 DOI： 10.3788/fgxb20133412.1662.

摘要

采用紫外光谱和循环伏安法，研究了丁二酰化壳寡糖稀土配合物（BCS-La、BCS-Nd）与鲱鱼精DNA之间的作用方式。BCS-La、BCS-Nd的存在导致Fe（CN）

3-/4-

探针分子峰电流下降，式量电位正移，表明BCS-La、BCS-Nd和探针分子与DNA之间存在竞争性作用；BCS-La和BCS-Nd分子都是通过插入方式与DNA相互作用。在一定的扫描速率范围内（0.01~0.2 V/s），在BCS-La或BCS-Nd参与的条件下，Fe（CN）

3-/4-

在Au/DNA电极上的反应受吸附控制。BCS-La和BCS-Nd分别使得鲱鱼精DNA的特征峰产生明显的减色效应，最大吸收峰峰位红移，进一步表明BCS-La和BCS-Nd分别以插入方式与鲱鱼精DNA发生相互作用，导致DNA分子的构象变化。BCS-La与DNA的结合比为：

（BCS-La）：

（DNA）=2：1；

（BCS-Nd）：

（DNA）=6：1。

Abstract

The interaction between succinic-oligochitosan-rare earth complexes (BCS-La and BCS-Nd) and herring sperm DNA was investigated by cyclic voltammetry and UV-Vis spectra. The results indicated as follows. Firstly

the peak current of probe molecule Fe(CN)

3-/4-

at Au/DNA electrodes obviously decreased and the peak potential shifted positively because of the existences of complexes. All above these showed that there was a competitive effect between complexes and probe molecule with herring sperm DNA

which revealed that a binding mode intercalation was interacted between complexes and herring sperm DNA. The reaction of Fe(CN)

3-/4-

at Au/DNA electrodes was controlled by adsorption mechanism in the range of scan rate from 0.01 V/s to 0.2 V/s. Secondly

the absorption intensity of herring sperm DNA decreased with the adding of complexes

and the maximum absorption peak had a red shift

which further illustrated that a binding mode intercalation was interacted between complexes and herring sperm DNA

thus led to the change of DNA conformation. The combining ratio of

(BCS-La) and

(BCS-Nd) with DNA was 2:1 and 6:1

respectively.

关键词

Keywords

references

Smits K M, Schouten J S, Smits L J, et al. A review on the design and reporting of studies on drug-gene interaction[J]. J. Clin. Epidemiol., 2005, 58(7):651-654.[2] Li X F, Feng X Q, Yang S, et al. Synthesis and interaction of succinic-oligochitosan-Eu(Ⅲ) complex with bovine serum albumin[J]. Chin. J. Lumin.(发光学报), 2012, 33(8):905-909 (in Chinese).[3] Li X F, Feng X Q, Yang S, et al. Synthesis and interaction of succinic-oligochitosan-La (Ⅲ) complex with bovine serum albumin[J]. Chin. J. Spectrosc. Lab.(光谱实验室), 2013, 30(3):1211-1215 (in Chinese).[4] Hu Q, Zhang K J, Jin H L, et al. Electrochemical investigation on the interaction of benzene sulfonyl 5-fluorouracil derivatives with double-stranded DNA and G-quadruplex DNA[J]. Scientia Sinica(Chimica)(中国科学:化学), 2012, 42(6):792-798 (in Chinese).[5] Patiente N, Sierra S, Airaksinen A. Action of mutagenic agents and antiviral inhibitors on foot-and-mouth disease virus[J]. Virus Res., 2005, 107(2):183-193.[6] Hulme A T, Price S L, Tocher D A. A new polymorph of 5-fluorouracil found following computational crystal structure predictions[J]. J. Am. Chem. Soc., 2007, 127(4):1117-1121.[7] Jarugula V R, Boudinot F D. High-performance liquid chromatographic determination of 5-fluorouracil and its prodrugs, tegafur and 4-deoxy-5-fluorouracil in rat plasma[J]. J. Chromatogr. B, 1996, 677(1):199-204.[8] Erkkila K E, Odom D T, Barton J K. Recognition and reaction of metallointercalators with DNA[J]. Chem. Rev., 1999, 99(9):2777-2796.[9] Du J Y, Huang X H, Xu F, et al. Spectral study on the interaction mechanism between thionine and calf thymus DNA[J]. Spectrosc. Spect. Anal.(光谱学与光谱分析), 2005, 25(9):1435-1439 (in Chinese).[10] Li H B, Ta H G, Wang X M, et al. Study on interaction between hematoporphyrin dihydrochloride and herring sperm DNA by spectroscopy[J]. Acta Optica Sinica (光学学报), 2008, 28(10):2016-2019 (in Chinese).[11] Wang X M, Li H B, Liu H P, et al. Interaction between Sm(Ⅲ)(MTB)2 metal complex and herring sperm DNA[J]. Acta Chimica Sinica (化学学报), 2006, 64(20):2115-2119 (in Chinese).[12] Chen X D, Gao J, Ding H Q. Infrared spectroscopy for non-invasive blood glucose monitoring (Invited)[J]. Chin. Opt. (中国光学), 2012, 5(4):317-326 (in Chinese).

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

8-羟基喹啉-山梨酸稀土配合物与鲱鱼精DNA相互作用的光谱研究

铽与苯甲酸及其衍生物配合物的合成与发光特性

铕与苯甲酸及其衍生物配合物的光谱分析

光致发光气凝胶研究进展

稀土离子掺杂钙钛矿纳米晶的光学性质和应用