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1. 华侨大学 生物医学学院,福建 泉州,362021
2. 华侨大学 分子药物教育部工程研究中心,福建 泉州,362021
收稿日期:2013-06-21,
修回日期:2013-08-17,
纸质出版日期:2013-11-10
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解丽娟, 李建民. 7-二乙胺基-3-(2'-吡啶)香豆素的新用途:溶酶体荧光探针[J]. 发光学报, 2013,34(11): 1538-1543
XIE Li-juan, LI Jian-min. A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin: As Lysosome Fluorescence Probe[J]. Chinese Journal of Luminescence, 2013,34(11): 1538-1543
解丽娟, 李建民. 7-二乙胺基-3-(2'-吡啶)香豆素的新用途:溶酶体荧光探针[J]. 发光学报, 2013,34(11): 1538-1543 DOI: 10.3788/fgxb20133411.1538.
XIE Li-juan, LI Jian-min. A New Use of 7-(diethylamino)-3-(pyridin-2-yl)coumarin: As Lysosome Fluorescence Probe[J]. Chinese Journal of Luminescence, 2013,34(11): 1538-1543 DOI: 10.3788/fgxb20133411.1538.
开发溶酶体荧光探针对于研究与溶酶体相关的细胞生理、病理活动具有重要的理论意义及实际应用价值。7-二乙胺基-3-(2'-吡啶)香豆素 (L3) 是一种弱碱性化合物
易于累积在酸性溶酶体中
有作为溶酶体荧光探针的潜质。本文详细研究了pH值对L3光谱性质的影响及其溶酶体定位成像能力。研究结果表明
该化合物具有一定的溶酶体定位能力
可以作为溶酶体荧光探针先导化合物。
The study of lysosomal fluorescence probe has important theoretical significance and practical value for cell physiological and pathological research associated with lysosome. As a weak basic compound which prefer to the acid lysosome
7-(diethylamino)-3-(pyridin-2-yl)coumarin (L3) may be a potential lysosome -targetable fluorescent probe. The relationship between pH value and the spectroscopic properties of L3 were investigated as well as lysosome-targetable fluorescent imaging in this study. The results show that L3 can be used as a lead compounds for lysosome-targetable fluorescent probe.
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