Nakamura K, Kuga H, Morisaki T, et al. Simulated microgravity culture system for a 3-D carcinoma tissue model [J]. Biotechniques, 2002, 33(5):1068-1070.[2] Wang X, Wei G, Yu W, et al. Scalable producing embryoid bodies by rotary cell culture system and constructing engineered cardiac tissue with ESDerived cardiomyocytes in vitro [J]. Biotechnol. Prog., 2006, 22(3):811-818.[3] Martin A, Zhou A, Gordon R E, et al. Thyroid organoid formation in simulated microgravity: Influence of keratinocyte growth factor [J]. Thyroid, 2000, 10(6):481-487.[4] Draux F, Jeannesson P, Beljebbar A, et al. Raman spectral imaging of single living cancer cells: A preliminary study [J]. Analyst, 2009, 134(3):542-548.[5] Kang L L, Huang Y X, Luo M. Confocal Raman microscopy on single living young and old erythrocytes [J]. Spectrosc. Spect. Anal.(光谱学与光谱分析), 2008, 28(10):2343-2347 (in Chinese).[6] Schrader B, Schulz H, Andreev G N, et al. On-destructive NIR-FT-Raman spectroscopy of plant and animal tissues, of food and works of art [J]. Talanta, 2000, 53(1):35-45.[7] Xie C A, Li Y Q. Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques [J]. Appl. Phys., 2003, 93(5):2982-2986.[8] Xie C A, Li Y Q, Tang W J. Study of dynamical process of heat denaturation in optically trapped single microorganisms by near-infrared Raman spectroscopy [J]. Appl. Phys., 2003, 94(9):6138-6142.[9] Potten C S, Loeffler M. Stem cells: Attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt [J]. Development, 1990, 110(4):1001-1020.[10] Fischbach C, Kong H J, Hsiong S X, et al. Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement [J]. Proc. Natl. Acad. Sci. USA, 2009, 106(2):399-404.[11] Kinasiewiczi A, Kawiak J, Werynski A. 3D matrigel culture improves differentiated functions of HepG2 cells in vitro [J]. Biocybern. Biomed. Eng., 2006, 26(4):47-55.[12] Fisher K E, Pop A, Koh W, et al. Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent [J]. Mol. Cancer, 2006, 5:69-72.