改进荧光法研究氟洛芬与牛血清白蛋白的相互作用

种宝红; 刘保生; 李志云; 曹世娜

doi:10.3788/fgxb20133410.1380

您当前的位置：

首页 >

文章列表页 >

改进荧光法研究氟洛芬与牛血清白蛋白的相互作用

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 改进荧光法研究氟洛芬与牛血清白蛋白的相互作用
- Modification on The Interaction Between Bovine Serum Albumin and Fluprofen by Improved Fluorescence Spectroscopy
- 发光学报 2013年34卷第10期页码：1380-1385
- 作者机构：
  
  河北大学化学与环境科学学院, 河北保定 071002
- 作者简介：
- 基金信息：
  
  国家自然科学基金(20675024)();河北省重点基础研究项目(10967126D)资助()
- DOI：10.3788/fgxb20133410.1380
  中图分类号： O657.3
- 收稿日期：2013-06-07，
  
  修回日期：2013-07-24，
  
  纸质出版日期：2013-10-10
- 稿件说明：
移动端阅览
种宝红, 刘保生, 李志云, 曹世娜. 改进荧光法研究氟洛芬与牛血清白蛋白的相互作用[J]. 发光学报, 2013,34(10): 1380-1385

CHONG Bao-hong, LIU Bao-sheng, LI Zhi-yun, CAO Shi-na. Modification on The Interaction Between Bovine Serum Albumin and Fluprofen by Improved Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2013,34(10): 1380-1385
种宝红, 刘保生, 李志云, 曹世娜. 改进荧光法研究氟洛芬与牛血清白蛋白的相互作用[J]. 发光学报, 2013,34(10): 1380-1385 DOI： 10.3788/fgxb20133410.1380.

CHONG Bao-hong, LIU Bao-sheng, LI Zhi-yun, CAO Shi-na. Modification on The Interaction Between Bovine Serum Albumin and Fluprofen by Improved Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2013,34(10): 1380-1385 DOI： 10.3788/fgxb20133410.1380.

摘要

为解决经典荧光光谱法在研究药物与蛋白结合机理中存在的缺陷

以蛋白与兽用药氟洛芬(FF)的结合为例

在生理条件(pH=7.40 的Tris-HCl缓冲溶液)下

以氟洛芬为荧光检测对象、牛血清白蛋白(BSA)为猝灭剂

通过改进荧光研究方法研究了药物与蛋白结合机理。结果表明

FF与BSA之间发生了静态猝灭

主要通过静电引力结合

其结合位点数约为1

Hill系数

略大于1

表现为弱的正协同作用。同时运用紫外吸收光谱法对该方法进行了验证

表明改进荧光法能更准确和全面地表征蛋白和药物的相互作用。

Abstract

In the Tris-HCl buffer solution which pH was 7.40

the interaction of bovine serum albumin with fluprofen was investigated by classical fluorescence spectroscopy and improved fluorescence spectroscopy at 293

303 and 310 K

the conclusions of the two methods were consistent. The results showed that fluprofen could quench the intrinsic fluorescence of bovine serum albumin

and the quenching mechanism was a static quenching process. The static electricity forces attraction played an important role on the conjugation reaction between BSA and fluprofen

the number of binding site (

) in the binary system was approximately to 1. The values of Hill's coefficients were more than 1

which indicated very weakly positive cooperativity in bovine serum albumin-fluprofen system. The binding constant (

) that obtained by improved fluorescence spectroscopy was much larger than the one obtained by classical fluorescence spectroscopy

which indicated that it was more accurate and reasonable when regarding the drug as the research object. At last

the scientificalness of the new method based on improved fluorescence spectroscopy was verified by ultraviolet spectroscopy.

关键词

Keywords

references

Liu Y M, Li G Z, Sun X F. Study on the interaction between colchicine and bovine serum albumins by fluorescence method [J]. Anal. Chem.(分析化学), 2004, 32(5):615-618 (in Chinese).[2] Smyk B. Fluorescence study of sinapic acid interatection with bovine serum albumin and egg albumin [J]. J. Fluoresc., 2003, 30(3):229-233.[3] Liu B S, Yang C, Yan X N, et al. Study on the conjugation mechamism of colistin sulfate with bovine serum albumin and effect of the metal ions on the reaction [J]. J. Lumin., 2012, 132(12):1133-1138.[4] Huang R, Xia Z N, Gong P. Study on the interaction of serum albumin and micromolecule compounds by modified fluorescence spectra [J]. Spectrosc. Spectral Anal.(光谱学与光谱分析), 2008, 28(1):161-164 (in Chinese).[5] Ding F, Chen J H, Jiang S X. Research progress in the fluprofen new antimicrobial agents [J]. China Animal Health Inspection (中国动物检疫), 2010, 27(2):66-68 (in Chinese).[6] Liu J Q, Tian J N, Zhang J Y, et al. Interaction of magnolol with bovine serum albumin: A fluorescence-quenching study [J]. Anal. Bioanal. Chem., 2003, 376(6):864-867.[7] Liu B S, Yan X N, Yang C, et al. Studies on the interaction between norfloxacin and ofloxacin in prescence of bovine serum albumin by fluorescence spectrum methods [J]. J. Hebei Univ.(Natural Science Edition) (河北大学学报: 自然科学版), 2011, 31(3):262-266 (in Chinese).[8] Li X Y. Interaction of metronidazole with bovine serum albumin by using fluorescence and resonance light scattering spectra [J]. Acta. Phys. Chim. Sinica (物理化学学报), 2007, 23(2):262-267 (in Chinese).[9] Feng X Z, Lin Z, Yang L J, et al. Investigation of the interaction between acridine orange and bovine serum albumin [J]. Talanta, 1998, 47(5):1223-1229.[10] Bi S Y, Sun Y T, Qiao C Y, et al. Binding of several anti-tumor drugs to bovine serum albumin: Fluorescence study [J]. J. Lumin., 2009, 129(5):541-547.[11] Liu Y, Xie M X, Kang J. Influence of total saponins of panax notoginseng on the conformation of BSA [J]. Acta. Chim. Sinica (分析化学), 2001, 61(8):1305-1310 (in Chinese).[12] Chang X J, Huang Y, He Q. Study on the interaction between Ir(Ⅳ)and gamma seroglobulinum humanum [J]. Acta. Chim. Sinica (化学学报), 2005, 63(3):223-228 (in Chinese).[13] Song Y M, Liu Z, Wang K J, et al. Interaction of the ternary complexes with anticoagulant property of iron, copper with human serum albumin [J]. Acta. Chim. Sinica (化学学报), 2010, 68(21):2191-2198 (in Chinese).[14] Liu B S, Liu Z C, Gao J. Interaction between erythromycin and bovine serum albumins by fluorescence method [J]. J. Hebei Univ.(Natural Science Edition) (河北大学学报: 自然科学版), 2005, 25(4):380-382 (in Chinese).[15] Li L W, Wang D D, Sun D Z, et al. Thermodynamic study on interaction between anti-tumor drug 5-fluorouracil and human serum albumin [J]. Acta. Chim. Sinica (化学学报), 2007, 65(27):2853-2857 (in Chinese).[16] Liu B S, Yang C, Wang J. Luminescence mechanism study of the conjugation reaction between cefpirome sulfate and bovine serum albumin [J]. Chin. J. Lumin.(发光学报), 2011, 32(3):293-299 (in Chinese).[17] Liang H, Bian H D, Tu C Q, et al. Binding equilibrium study between La(Ⅲ) and HSA or BSA [J]. Chem. J. Chin. Univ.(高等化学学报), 2001, 22(1):21-25 (in Chinese).

浏览量

156

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

光谱法及分子对接技术研究黄体酮与牛血清白蛋白的结合机制

胡椒酸乙酯与牛血清白蛋白的相互作用及金属离子的影响

头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响

丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用

头孢噻肟钠和氯霉素与牛血清白蛋白相互作用的荧光光谱分析