以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法

曹世娜; 刘保生; 李志云; 种宝红

doi:10.3788/fgxb20133407.0930

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以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法
- A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe
- 发光学报 2013年34卷第7期页码：930-934
- 作者机构：
  
  河北大学化学与环境科学学院,河北保定,071002
- 作者简介：
- 基金信息：
  
  国家自然科学基金(20675024)();河北省重点基础研究项目(10967126D)资助项目()
- DOI：10.3788/fgxb20133407.0930
  中图分类号： O657.3
- 收稿日期：2013-04-09，
  
  修回日期：2013-06-01，
  
  纸质出版日期：2013-07-10
- 稿件说明：
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曹世娜, 刘保生, 李志云, 种宝红. 以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法[J]. 发光学报, 2013,34(7): 930-934

CAO Shi-na, LIU Bao-sheng, LI Zhi-yun, CHONG Bao-hong. A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2013,34(7): 930-934
曹世娜, 刘保生, 李志云, 种宝红. 以牛血清白蛋白为荧光探针的格列吡嗪含量测定方法[J]. 发光学报, 2013,34(7): 930-934 DOI： 10.3788/fgxb20133407.0930.

CAO Shi-na, LIU Bao-sheng, LI Zhi-yun, CHONG Bao-hong. A Determination Method of Glipizide Content with Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2013,34(7): 930-934 DOI： 10.3788/fgxb20133407.0930.

摘要

在pH=7.40 的Tris-HCl缓冲溶液中

牛血清白蛋白(BSA)与格列吡嗪(Glipizide)的相互作用会使BSA荧光有规律地发生猝灭

据此建立了以BSA为探针的Glipizide含量测定方法。Glipizide浓度在0.25~20 mol/L范围内与荧光强度呈现良好的线性关系

方法的检出限为0.071 mol/L。对于片剂中Glipizide含量的测定

平行6次测定的相对标准偏差为1.29%~2.80%

回收率为86.79%~96.43%。

Abstract

In Tris-HCl buffer solution(pH=7.40)

the reaction between bovine serum albumin (BSA) and Glipizide can lead the fluorescence of BSA quenching regularly. Based on this pheno-menon

a new method that determining the content of Glipizide was established

in which BSA was used as the fluorescent probe. The concentration of Glipizide presented a nice liner relation with the fluorescence intensity from 0.25 to 20 mol/L. The detection limit was 0.071 mol/L. The method was applied to analysis the Glipizide content in drugs samples

and the RSD was from 1.29% to 2.80%

the recovery rate was from 86.79% to 96.43% with six parallel determinations.

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references

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