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1. 郑州大学 药学院,河南 郑州,450001
2. 郑州大学 化学与分子工程学院,河南 郑州,450001
3. 河南工业大学 化学化工学院,河南 郑州,450001
收稿日期:2013-01-05,
修回日期:2013-03-22,
纸质出版日期:2013-05-10
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雷利芳, 游静, 曾华金, 杨冉, 屈凌波. 微孔板结合化学发光法快速测定覆盆子中总糖蛋白的抗氧化活性[J]. 发光学报, 2013,34(5): 650-655
LEI Li-fang, YOU Jing, ZENG Hua-jin, YANG Ran, QU Ling-bo. Rapid Determination of Antioxidant Activities of Glycoprotein of <em>Rubus chingii Hu</em>. by Chemiluminescence Method Coupled with Microwell Plate[J]. Chinese Journal of Luminescence, 2013,34(5): 650-655
雷利芳, 游静, 曾华金, 杨冉, 屈凌波. 微孔板结合化学发光法快速测定覆盆子中总糖蛋白的抗氧化活性[J]. 发光学报, 2013,34(5): 650-655 DOI: 10.3788/fgxb20133405.0650.
LEI Li-fang, YOU Jing, ZENG Hua-jin, YANG Ran, QU Ling-bo. Rapid Determination of Antioxidant Activities of Glycoprotein of <em>Rubus chingii Hu</em>. by Chemiluminescence Method Coupled with Microwell Plate[J]. Chinese Journal of Luminescence, 2013,34(5): 650-655 DOI: 10.3788/fgxb20133405.0650.
在碱性条件下
覆盆子总糖蛋白对鲁米诺-过氢化氢、鲁米诺-邻苯三酚-氢氧化钠和鲁米诺-硫酸铜-过氧化氢化学发光体系具有明显的抑制作用。基于这一特点
本文将微孔板与化学发光法结合
在优化条件下
建立了覆盆子总糖蛋白清除H
2
O
2
、O
2-
和OH自由基能力的快速检测方法
结果表明该方法具有样品用量少、操作简单、检测速度快等优点
适用于药物中有效成分的活性检测及筛选。
Based on the phenomenon that glycoprotein can obviously inhibit the chemical luminescence (CL) signal of luminol-H
2
O
2
system
luminol-pyrogallol-sodium hydroxide system and luminol-H
2
O
2
-copper sulfate system in basic medium
a simple and rapid chemiluminescence method int egrated with microwell plate was developed for evaluating of the scavenge effects of glycoprotein of
Rubus chingii Hu
. on hydrogen peroxide (H
2
O
2
)
superoxide anion (O
2-
) and hydroxyl radical (OH). The experiment data indicated the proposed method described in this paper has proved to be fairly simple
reproducible and rapid and would be a valuable tool for fast screening of antioxidant activity of herbal.
Shuichi K, Hiroyuki A, Hirohide T. Isolation of antidiabetic components from white-skinned sweet potato(Ipomoea batatas L.) [J]. Biosci., Biotechnol. Biochem., 2001, 65(1):109-114.[2] Li Y N, Zhao M M, Peng Z Y, et al. Study on the isolation and purification of sweet potato glycoprotein from the cultivar-Beijing 2 and its antilipemic function [J]. Food Science (食品科学), 2003, 4(1):118-121 (in Chinese).[3] Lim K T, Son Y O, Lee J C, et al. Effects of glycoprotein from Ulmus davidiana Nakay on hydroxyl radical induced cytotoxicity and on activation of nuclear factor-B and activator protein-1 in cultured mouse primary thymocytes [J]. Korean J. Food Sci. Biotechnol., 2002, 11(1):172-178.[4] Oh P S, Lim K T. Antioxidant activity of dioscorea batatas decne glycoprotein [J]. Eur. Food Res. Technol., 2008, 226(3): 507-515.[5] Miyazaki Y, Kusano S, Doi H, et al. Effects on immune response of antidiabetic ingredients from white-skinned sweet potato (Ipomoea batatas L.) [J]. Nutrition, 2005, 21(3):358-362.[6] Sasaki T, Uchida H. Antitumor activity and immunomodulatory effect of glycoprotein fraction from scall oppartinopecten yessoensis [J]. Comp. Biochem. Phys., 1992, 103(1):102-106.[7] Li X W, Zhang K C, Xu G X, et al. Experimental research on prescription for anti-aging and prolonging life span [J]. J. Hunan College of Traditional Chinese Medicine (湖南中医药大学学报), 1991, 11(3):33-36 (in Chinese).[8] Niu F G, Wang J P, Wang F, et al. Study on antioxidation effect in vivo of glycoprotein from extract of Rubus chingii Hu[J]. Science and Technology of Food Industry (食品工业科技), 2010, 31(12):134-136 (in Chinese).[9] Wang F, Yang H J, Duan Y F. Study on the extraction technology of glycoprotein from Rubus chingii Hu.[J]. J. Anhui Agricultural Sci.(安徽农业科学), 2009, 37(8):3756-3758 (in Chinese).
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