利用延迟荧光光谱F730/F685的逆境胁迫生理检测方法

李瑛; 许文海

doi:10.3788/fgxb20113207.0740

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利用延迟荧光光谱F₇₃₀/F₆₈₅的逆境胁迫生理检测方法

光谱分析 | 更新时间：2020-08-12

- 利用延迟荧光光谱F₇₃₀/F₆₈₅的逆境胁迫生理检测方法
- Detection of Stress Physiology of Plant Using F₇₃₀/F₆₈₅ Ratio of Delayed Fluorescence Spectrum
- 发光学报 2011年32卷第7期页码：740-747
- 作者机构：
  
  大连海事大学信息科学技术学院,辽宁大连,116026
- 作者简介：
- 基金信息：
  
  国家自然科学基金(60774056)();教育部新教师基金(200801511013)资助项目()
- DOI：10.3788/fgxb20113207.0740
  中图分类号： Q632
- 收稿日期：2011-02-15，
  
  修回日期：2011-04-20，
  
  网络出版日期：2011-07-22，
  
  纸质出版日期：2011-07-22
- 稿件说明：
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李瑛, 许文海. 利用延迟荧光光谱F730/F685的逆境胁迫生理检测方法[J]. 发光学报, 2011,32(7): 740-747

LI Ying, XU Wen-hai. Detection of Stress Physiology of Plant Using F730/F685 Ratio of Delayed Fluorescence Spectrum[J]. Chinese Journal of Luminescence, 2011,32(7): 740-747
李瑛, 许文海. 利用延迟荧光光谱F730/F685的逆境胁迫生理检测方法[J]. 发光学报, 2011,32(7): 740-747 DOI： 10.3788/fgxb20113207.0740.

LI Ying, XU Wen-hai. Detection of Stress Physiology of Plant Using F730/F685 Ratio of Delayed Fluorescence Spectrum[J]. Chinese Journal of Luminescence, 2011,32(7): 740-747 DOI： 10.3788/fgxb20113207.0740.

摘要

以玉米98-2为样品

实验分析了高温、盐及紫外UV-B胁迫下

样品离体叶片延迟荧光发射光谱的变化。标记685 nm主峰带的最高峰值为

685

730 nm次峰带的最高峰值为

730

实验发现不同的逆境胁迫对比值

730

685

均产生了显著的影响。结合样品在相同逆境下叶绿素荧光参数

以及DF强度的检测实验

结果表明

无论是在哪种逆境胁迫下

无论是对胁迫程度还是胁迫时间的响应特性上

730

685

、DF强度与

均有着较好的一致性

线性拟合相似度大于0.960 9。因此

通过对本文实验结果的分析认为

延迟荧光光谱峰值比

730

685

的逆境胁迫响应与叶片受胁迫影响的程度密切相关

且可能是一种检测植物逆境光合生理的新方法。

Abstract

Delayed fluorescece (DF) is intrinsically related to the photosynthesis process

and it is a good method of researching the effect of various chemical and physical factors in vivo and in vitro on photosynthetic performance of plants. In this paper

the detection of DF emission spectra under the stress (heat

salt and UV-B) have been investigated in maize (98-2) sample. The maximal values of DF emission spectra at 685 nm and 730 nm were marked as

685

and

730

respectively. After comparative measurement of

730

685

ratio

DF intensity and

the linear fitting results shown that the two parameters has a good linear correlation (

0.960 9). The experimental results suggest that the degree of plant photosynthetic physiology damage whether caused by different concentration or different stress time can be effectively measured by

730

685

. Moreover

the responses of

730

685

ratio of DF emission spectra to the stress could be a new and useful judgment to identify and evaluate the stress damage of the samples.

关键词

Keywords

references

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