金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测

孙桂敏; 杨培慧; 孙俊环; 蔡继业

doi:10.3788/fgxb20113206.0636

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金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测

光谱分析 | 更新时间：2020-08-12

- 金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测
- Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM
- 发光学报 2011年32卷第6期页码：636-641
- 作者机构：
  
  1. 暨南大学化学系,广东广州,510632
  2. 暨南大学光电信息与传感技术广东普通高校重点实验室,广东广州,510632
  3. 重庆市农业科学院重庆,401329
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30828023,30872404)();973重大项目(2010CB833603)资助项目()
- DOI：10.3788/fgxb20113206.0636
  中图分类号： O482.31
- 收稿日期：2011-01-25，
  
  修回日期：2011-02-24，
  
  网络出版日期：2011-06-22，
  
  纸质出版日期：2011-06-22
- 稿件说明：
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孙桂敏, 杨培慧, 孙俊环, 蔡继业. 金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测[J]. 发光学报, 2011,32(6): 636-641

SUN Gui-min, YANG Pei-hui, SUN Jun-huan, CAI Ji-ye. Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM[J]. Chinese Journal of Luminescence, 2011,32(6): 636-641
孙桂敏, 杨培慧, 孙俊环, 蔡继业. 金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测[J]. 发光学报, 2011,32(6): 636-641 DOI： 10.3788/fgxb20113206.0636.

SUN Gui-min, YANG Pei-hui, SUN Jun-huan, CAI Ji-ye. Fluorescence Imaging of Hepatocellular Carcinoma Cells Labeled with Gold Nanorods and Detection by AFM[J]. Chinese Journal of Luminescence, 2011,32(6): 636-641 DOI： 10.3788/fgxb20113206.0636.

摘要

金纳米棒具有独特的光学性质

在生物医学领域有着广泛而重要的应用前景。本文制备了长径比为8 ∶ 1的金纳米棒

其在480 nm波长激发下

在560 nm和707 nm波长处有两个荧光发射峰。基于金纳米棒的荧光性质

将其标记于HepG2人肝癌细胞表面

利用激光扫描共聚焦显微镜对标记后的细胞进行荧光成像。在488 nm激发下

获得了绿色和红色两种颜色的荧光图像

并进一步用高分辨率的原子力显微镜探测了GNRs标记后细胞形貌及机械性质的变化。

Abstract

Due to the unique and tunable optical properties

gold nanorods have potential and important applications in the biomedical field. In this paper

gold nanorods with the aspect ratio 8 were prepared

an outstanding fluorescence feature of the gold nanorods excited at 480 nm was observed in terms of a strong fluorescence emission at 707 nm and a relatively weak fluorescence at 560 nm. Based on their fluorescence properties

HepG2 cells were labeled by this type of gold nanorods

and were studied by using laser scanning confocal microscope. Green and red fluorescence images of gold nanorods were collected under the excitation of 488 nm. Furthermore

high-resolution atomic force microscopy was used to obtain the morphology and mechanical properties changes of the HepGz cells.

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Keywords

references

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