基于光子技术的UV-B辐射对植物细胞伤害评价方法

习岗; 张晓辉; 刘锴; 李少华

doi:10.3788/fgxb20113204.0398

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基于光子技术的UV-B辐射对植物细胞伤害评价方法

光谱分析 | 更新时间：2020-08-12

- 基于光子技术的UV-B辐射对植物细胞伤害评价方法
- Evaluation Method of UV-B Radiation Damage on Plant Cell Based on Photonic Technology
- 发光学报 2011年32卷第4期页码：398-403
- 作者机构：
  
  1. 西安理工大学应用物理系,陕西西安,710048
  2. 西安理工大学电子系, 陕西西安 710048
- 作者简介：
- 基金信息：
  
  国家自然科学基金(50977079)();陕西省教育厅专项科研计划(09JK667)资助项目()
- DOI：10.3788/fgxb20113204.0398
  中图分类号： Q632;O482.31
- 收稿日期：2010-09-23，
  
  修回日期：2010-11-15，
  
  网络出版日期：2011-04-22，
  
  纸质出版日期：2011-04-22
- 稿件说明：
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习岗, 张晓辉, 刘锴, 李少华. 基于光子技术的UV-B辐射对植物细胞伤害评价方法[J]. 发光学报, 2011,32(4): 398-403

XI Gang, ZHANG Xiao-hui, LIU Kai, LI Shao-hua. Evaluation Method of UV-B Radiation Damage on Plant Cell Based on Photonic Technology[J]. Chinese Journal of Luminescence, 2011,32(4): 398-403
习岗, 张晓辉, 刘锴, 李少华. 基于光子技术的UV-B辐射对植物细胞伤害评价方法[J]. 发光学报, 2011,32(4): 398-403 DOI： 10.3788/fgxb20113204.0398.

XI Gang, ZHANG Xiao-hui, LIU Kai, LI Shao-hua. Evaluation Method of UV-B Radiation Damage on Plant Cell Based on Photonic Technology[J]. Chinese Journal of Luminescence, 2011,32(4): 398-403 DOI： 10.3788/fgxb20113204.0398.

摘要

用50 W/cm

UV-B连续辐射大豆愈伤组织16 h

发现大豆愈伤组织的自发发光强度逐渐增长

延迟发光呈现衰减的趋势。通过对延迟发光动力学过程的分析

得到了UV-B辐射下大豆愈伤组织延迟发光的特征参数积分强度

I(T)、

初始光子数

、

相干时间

和衰减参数

的变化规律。结果表明

大豆愈伤组织延迟发光初始光子数

、

相干时间

和衰减参数

随着UV-B辐射时间的延长逐渐减小

延迟发光积分强度

I(T)

与初始光子数

成极好的正相关。根据生物超弱光子辐射的意义定义了描述细胞生命状态有序程度的序参量

发现在50 W/cm

UV-B连续辐射下

大豆愈伤组织序参量逐渐减小

大豆愈伤组织序参量的变化很好地反映了UV-B辐射对组织细胞的伤害。

Abstract

In order to evaluate the method of UV-B radiation damage on plant cell by photonic technology

soybean callus was radiated by 50 W/cm

UV-B for 16 h. Ultraweak photon emission from soybean callus was collected and analyzed. The results showed that strength of spontaneous luminescence (SL) in ultraweak photon emission of soybean callus increased and delayed luminescence (DL) decreased gradually. Through the analyses of dynamic presses of DL

the change rules of dynamic parameters of DL such as integrated inten-sity

I(T)

initial

correlation time

and decay constant

were obtained. It was indicated that integrated intensity

I(T)

initial photon number

correlation time

and decay constant

decreased with the UV-B radia- tion time

and

I(T)

was positive correlation to initial photon number

. The order parameter used to describe order degree of cell was defined according to biological signification of ultraweak photon emission. The study found that the changes of the order parameter of soybean callus under the action of UV-B radiation reflected the damage of UV-B radiation on cell. So

the damage of UV-B radiation on plant cell could be expressed by the order parameter based on ultraweak photon emission of cell.

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Keywords

references

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