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基于延迟荧光光谱F685/F730温度响应曲线的植物耐热性评价
The Application of The F685/F730 Ratio of Delayed Fluorescence Spectrum Temperature Curve in Heat Tolerance Evaluation
- 发光学报 2011年32卷第1期 页码：94-99
- 作者机构：
1. 大连海事大学 信息科学技术学院,辽宁 大连,116026
2. 华南师范大学 激光生命科学研究所,广东 广州,510631
- 作者简介：
- 基金信息：国家自然科学基金(60774056)();中央高校基本科研业务费专项基金(2009QN032)资助项目()
DOI：10.3788/fgxb20113201.0094
中图分类号： Q947.8;O482.31收稿日期：2010-04-19，
修回日期：2010-07-02，
网络出版日期：2011-01-22，
纸质出版日期：2011-01-22
- 稿件说明：
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李瑛, 许文海, 董丽丽, 邢达. 基于延迟荧光光谱<em>F</em><sub>685</sub>/<em>F</em><sub>730</sub>温度响应曲线的植物耐热性评价[J]. 发光学报, 2011,32(1): 94-99
LI Ying, XU Wen-hai, DONG Li-li, XING Da. The Application of The <em>F</em><sub>685</sub>/<em>F</em><sub>730</sub> Ratio of Delayed Fluorescence Spectrum Temperature Curve in Heat Tolerance Evaluation[J]. Chinese Journal of Luminescence, 2011,32(1): 94-99
李瑛, 许文海, 董丽丽, 邢达. 基于延迟荧光光谱<em>F</em><sub>685</sub>/<em>F</em><sub>730</sub>温度响应曲线的植物耐热性评价[J]. 发光学报, 2011,32(1): 94-99 DOI： 10.3788/fgxb20113201.0094.
LI Ying, XU Wen-hai, DONG Li-li, XING Da. The Application of The <em>F</em><sub>685</sub>/<em>F</em><sub>730</sub> Ratio of Delayed Fluorescence Spectrum Temperature Curve in Heat Tolerance Evaluation[J]. Chinese Journal of Luminescence, 2011,32(1): 94-99 DOI： 10.3788/fgxb20113201.0094.
摘要
以3种不同耐热型玉米植株叶片为样品
分析了在10~50 ℃温度胁迫下
样品离体叶片延迟荧光光谱的变化。实验发现:温度对延迟荧光光谱主峰685 nm的最高峰值强度(
F
685
)和次峰730 nm的最高峰值强度(
F
730
)的比值
F
685
/
F
730
产生了显著的影响。此外
不同耐热型样品在10 ℃胁迫下的
F
685
/
F
730
值(
V
10
)与50 ℃胁迫下的
F
685
/
F
730
值(
V
50
)之间存在显著差异:不耐热样品叶片的
V
10
/
V
50
高于耐热的
越耐热的样品
V
10
/
V
50
值越小
且随着胁迫时间的延长
V
10
/
V
50
也逐渐减小。因此
通过对实验结果的分析认为
延迟荧光光谱
F
685
/
F
730
比值是随着温度胁迫规律变化的
F
685
/
F
730
比值的温度响应曲线有望成为一种鉴定和评价植物耐热性的新方法。
Abstract
In order to obtain the relationship between temperature-stress and delayed fluorescence (DF) emission spectra
in this paper
three different heat-resistant maizes were used as samples to experimentally study the plant DF emission DF spectra at different elevated temperatures (10~50 ℃). The experimental spectra were obtained by 660 nm excitation. Results showed that there are two distinct emission peaks in DF spectra
that is
the main peak at around 685 nm and the second peak at around 730 nm. The maximal values of DF emission spectra at 685 nm and 730 nm were marked as
F
685
and
F
730
respectively. The experimental results illustrated that the
F
685
/
F
730
ratio varied with the increase of temperature. It was also found that the
F
685
/
F
730
ratio at 10 ℃and 50 ℃
which was marked as
V
10
and
V
50
respectively
are obviously different. The
V
10
/
V
50
ratio depended on the heat-resisting property of the samples. These results indicated that the higher the heat-resisting property of the sample is
the lower the
V
10
/
V
50
ratio is. Additionally
the longer temperature stress time is
the lower
V
10
/
V
50
ratio is. Hence
we concluded that DF emission spectra vary with the temperature-stress
and the response curve of
F
685
/
F
730
ratios of different samples display a similar law. Moreover
the responses of
F
685
/
F
730
ratio of DF emission spectra to the temperature for the different heat-resistant Maize samples could be a new and useful judgment to identify and evaluate the heat-resisting property of maize.
关键词
Keywords
references
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