大豆愈伤组织超弱光子辐射的双指数模型

习岗; 杨运经; 李少华; 张艳

doi:10.3788/fgxb20113201.0087

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大豆愈伤组织超弱光子辐射的双指数模型

光谱分析 | 更新时间：2020-08-12

- 大豆愈伤组织超弱光子辐射的双指数模型
- Double-exponential Model of Ultraweak Photon Emission of Soybean Callus
- 发光学报 2011年32卷第1期页码：87-93
- 作者机构：
  
  1. 西安理工大学应用物理系,陕西西安,710048
  2. 西北农林科技大学应用物理系, 陕西杨凌 712100
- 作者简介：
- 基金信息：
  
  Project supported by National Natural Science Foundation of China(50977079)();Shaanx
- DOI：10.3788/fgxb20113201.0087
  中图分类号： Q632;O482.31
- 收稿日期：2010-01-07，
  
  修回日期：2010-05-13，
  
  网络出版日期：2011-01-22，
  
  纸质出版日期：2011-01-22
- 稿件说明：
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习岗, 杨运经, 李少华, 张艳. 大豆愈伤组织超弱光子辐射的双指数模型[J]. 发光学报, 2011,32(1): 87-93

XI Gang, YANG Yun-jing, LI Shao-hua, ZHANG Yan. Double-exponential Model of Ultraweak Photon Emission of Soybean Callus[J]. Chinese Journal of Luminescence, 2011,32(1): 87-93
习岗, 杨运经, 李少华, 张艳. 大豆愈伤组织超弱光子辐射的双指数模型[J]. 发光学报, 2011,32(1): 87-93 DOI： 10.3788/fgxb20113201.0087.

XI Gang, YANG Yun-jing, LI Shao-hua, ZHANG Yan. Double-exponential Model of Ultraweak Photon Emission of Soybean Callus[J]. Chinese Journal of Luminescence, 2011,32(1): 87-93 DOI： 10.3788/fgxb20113201.0087.

摘要

在研究生物超弱光子辐射机理的基础上

建立了大豆愈伤组织超弱光子辐射的双指数模型。用20 W/cm

的UV-B辐射处理大豆愈伤组织2 h

测定处理后4 d内的超弱光子辐射。结果显示

双指数模型准确描述了大豆愈伤组织的超弱光子辐射及其在UV-B辐射下的变化

表明大豆愈伤组织的超弱光子辐射由快项和慢项两个部分组成;通过对双指数模型的分析得到了大豆愈伤组织自发发光与延迟发光中的初始光子数、积分强度和快项与慢项两个部分的衰减参数

由这些发光信息可以实现对大豆愈伤组织超弱光子辐射变化的定量分析。研究结果还发现

在UV-B辐射处理后的4 d内

延迟发光的快项部分发生了剧烈变化

自发发光出现了相反的变化趋势

暗示大豆愈伤组织超弱光子辐射对UV-B辐射反应灵敏的是延迟发光中的快项部分

细胞膜脂过氧化可能是造成快项部分剧烈变化的原因。

Abstract

Double-exponential model of ultraweak photon emission of soybean callus was proposed based on the mechanism of ultraweak photon emission in biological system. Treated soybean callus by UV-B radiation(20 W/cm

)for 2 h

ultraweak photon emission of soybean callus treated by UV-B for 4 d after was measured. The results show that double-exponential model accurately describes the characteristics of ultraweak photon emission of soybean callus and its changes

delayed luminescence of ultraweak photon emission of soybean callus is composed by both fast and slow phase. Spontaneous luminescence in ultraweak photon emission of soybean callus and some parameters of delayed luminescence such as initial photon number

(0)

integrated intensity

I(T)

and decay parameters of delayed luminescence are obtained through analysis of the double-exponential model

the characteristics of ultraweak photon emission of soybean callus can be quantitative expressed by these parameters. The studies also find that there is an excellent positive correlation between initial photon number

(0) and integrated intensity

I(T)

and

(0) or

I(T)

can be used to measure the metabolic intensity of soybean callus. Induced by UV-B radiation(20 W/cm

) for 2 h

the decay parameter of fast phase in delayed luminescence decreases first and then increases

the change of spontaneous luminescence is opposite

it suggests that fast phase of delayed luminescence is sensitive to the UV-B radiation and membrane lipid peroxidation could be the reason of the drastic change of fast phase in delayed luminescence of soybean callus.

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references

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