皮肤晚期糖基化终末产物的自体荧光光谱特性

张龙; 朱灵; 王贻坤; 夏营威; 刘勇; 王安

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皮肤晚期糖基化终末产物的自体荧光光谱特性

光谱分析 | 更新时间：2020-08-12

- 皮肤晚期糖基化终末产物的自体荧光光谱特性
- Characteristics of Advanced Glycation End Products Self-fluorescence Spectra in Human Skin
- 发光学报 2011年32卷第9期页码：968-971
- 作者机构：
  
  中国科学院安徽光学精密机械研究所合肥,230031
- 作者简介：
- 基金信息：
  
  中国科学院知识创新工程人才专项基金(083RC11124)资助项目()
- DOI：
  中图分类号： O482.31
- 收稿日期：2011-03-17，
  
  修回日期：2011-04-25，
  
  网络出版日期：2011-09-22，
  
  纸质出版日期：2011-09-22
- 稿件说明：
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张龙, 朱灵, 王贻坤, 夏营威, 刘勇, 王安. 皮肤晚期糖基化终末产物的自体荧光光谱特性[J]. 发光学报, 2011,32(9): 968-971

ZHANG Long, ZHU Ling, WANG Yi-kun, XIA Ying-wei, LIU Yong, WANG An. Characteristics of Advanced Glycation End Products Self-fluorescence Spectra in Human Skin[J]. Chinese Journal of Luminescence, 2011,32(9): 968-971
张龙, 朱灵, 王贻坤, 夏营威, 刘勇, 王安. 皮肤晚期糖基化终末产物的自体荧光光谱特性[J]. 发光学报, 2011,32(9): 968-971 DOI：

ZHANG Long, ZHU Ling, WANG Yi-kun, XIA Ying-wei, LIU Yong, WANG An. Characteristics of Advanced Glycation End Products Self-fluorescence Spectra in Human Skin[J]. Chinese Journal of Luminescence, 2011,32(9): 968-971 DOI：

摘要

在分析晚期糖基化终末产物(Advanced glycation end products

AGEs)标准品三维荧光光谱和皮肤中AGEs自体荧光光谱的基础上

确定了皮肤中AGEs的最佳激发波长为370 nm。使用自行设计的皮肤荧光光谱检测装置对正常人和糖尿病患者皮肤AGEs的荧光光谱进行了检测

结果表明:在418

450

470 nm处存在3个峰值结构。对光谱归一化处理后发现

450 nm和470 nm处荧光与皮肤中AGEs含量密切相关。因此

使用370 nm激发的皮肤自体荧光可以定量分析AGEs浓度

并可用于AGEs相关疾病的临床诊断。

Abstract

In this study

three dimension fluorescence spectra of standard sample of Advanced Glycation End products (AGEs)

and skin self-fluorescence of AGEs are analyzed. The optimal excitation wavelength for skin AGEs is 370 nm. The skin self-fluorescence of control subjects and diabetic patients are measured by an instrumentation developed by ourselves. The results show that there are three obvious emission peaks at 418

450

470 nm

after normalizing the fluorescence spectra. It's distinctly that the peaks around 450 nm and 470 nm are correlated with the concentration of skin AGEs. Therefore

skin self-fluorescence excited by 370 nm can be utilized to quantitatively analyze the concentration of AGEs

and are also available for clinical diagnosing of correlated diseases.

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Keywords

references

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