硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理

刘保生; 杨超; 王晶; 薛春丽; 吕运开

doi:null

您当前的位置：

首页 >

文章列表页 >

硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理

光谱分析 | 更新时间：2020-08-12

- 硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理
- Luminescence Mechanism Study of The Conjugation Reaction between Cefpirome Sulfate and Bovine Serum Albumin
- 发光学报 2011年32卷第3期页码：293-299
- 作者机构：
  
  河北大学化学与环境科学学院药物化学与分子诊断教育部重点实验室,河北保定,071002
- 作者简介：
- 基金信息：
  
  国家自然科学基金(20675024)();河北省重点基础研究项目(10967126D)资助()
- DOI：
  中图分类号： O657.3;O482.31
- 收稿日期：2010-10-29，
  
  修回日期：2010-11-15，
  
  网络出版日期：2011-03-22，
  
  纸质出版日期：2011-03-22
- 稿件说明：
移动端阅览
刘保生, 杨超, 王晶, 薛春丽, 吕运开. 硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理[J]. 发光学报, 2011,32(3): 293-299

LIU Bao-sheng, YANG Chao, WANG Jing, XUE Chun-li, LU Yun-kai. Luminescence Mechanism Study of The Conjugation Reaction between Cefpirome Sulfate and Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(3): 293-299
刘保生, 杨超, 王晶, 薛春丽, 吕运开. 硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理[J]. 发光学报, 2011,32(3): 293-299 DOI：

LIU Bao-sheng, YANG Chao, WANG Jing, XUE Chun-li, LU Yun-kai. Luminescence Mechanism Study of The Conjugation Reaction between Cefpirome Sulfate and Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(3): 293-299 DOI：

摘要

在人生理条件下

利用荧光猝灭法、同步荧光法及共振光散射法

分别研究了不同温度下硫酸头孢匹罗(CPS)与牛血清白蛋白(BSA)间的结合反应。结果表明:随着CPS浓度的增加

BSA的荧光、共振散射光依次降低

其荧光猝灭为静态猝灭过程并伴随非辐射能量转移作用。反应的结合常数为10

数量级

结合位点数约为1;结合位点位于BSA的亚结构ⅡA中;结合距离

＜7 nm。CPS与BSA间作用力主要是静电引力;Hill系数

＜1

表明CPS有弱的负协同作用。同步荧光光谱表明CPS对BSA构象产生影响

使BSA腔内疏水环境的极性增强

疏水性减弱。

Abstract

In human physical conditions

the binding reaction between cefpirome sulfate (CPS) and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy

synchronous fluorescence spectroscopy and resonance light scattering (RLS) at different temperatures. Results showed that both fluorescence and RLS of BSA reduced with the increased concentration of CPS

and the effect between CPS and BSA was static fluorescence quenching process with Frester spectroscopy energy transfer. The scope of apparent binding constants (

) was ~10

; the corresponding binding site value (

) in the binary systems was 1; the binding distances (

) were much smaller than 7 nm and the primary binding site for CPS was located at site Ⅰ in sub-domain ⅡA of BSA. Besides

the electrostatic attraction plays an important role in the conjugation reaction of BSA and CPS. The values of Hills coefficients were less than 1

which indicated that there was some negative cooperative effect. Studies utilizing synchronous spectra showed that the conjugation reaction between CPS and BSA would affect the conformation of BSA

leading to the polarity around BSA strengthened and the hydrophobicity weakened.

关键词

Keywords

references

Mathias U, Jung M. Determination of drug-serum protein interactions via fluorescence polarization measurements [J]. Analytical and Bioanalytical Chemistry, 2007, 388 (5-6):1147-1156.[2] Sun Guanning, Wu Qiongzhu. Content and related substances determination of cefpirome sulfate by HPLC [J]. Pharmaceutical and Clinical Research (药学与临床研究), 2007, 15 (2):152-154 (in Chinese).[3] Bogdam S. Fluorescence study of sinapic acid interaction with bovine serum albumin and egg albumin [J]. J. Fluores-cence, 2003, 13 (4):349-356.[4] Zhang Guowen, Chen Xiuxia, Pan Junhui, et al. Thermodynamic analysis on the binding feature between morin and bovine serum albumin [J]. Journal of Nanchang University (南昌大学学报), 2008, 30 (3):229-233 (in Chinese).[5] Bai Haixin, Yang Cheng, Yang Xiurong. Study on the interaction of bovine serum albumin with Indo-1 by fluorescence method [J]. Chem. J. Chin. Univ. (高等学校化学学报), 2007, 28 (2):227-233 (in Chinese).[6] Steiner R F, Weinryb L. Exicited States of Protein and Nucleic Acid [M]. New York: Plenum Press, 1971:40.[7] Zhang Hongmei, Wang Yanqing, Qiu Jingxia, et al. Studies on the interaction between morin and bovine hemoglobin (BHb) by spectroscopic methods [J]. Chin. J. Lumin. (发光学报), 2007, 28 (4):566-572 (in English).[8] Wang Y Q, Zhang H M, Zhang G C, et al. Interaction of the flavonoid hesperidin with bovine serum albumin [J]. J. Lumin., 2007, 126 (1):211-218.[9] Liu Baosheng, Xue Chunli, Wang Jing, et al. Studies on the interaction between ciprofloxacin and ofloxacin in presence of bovine serum albumin by spectroscopic methods [J]. Chin. J. Lumin. (发光学报), 2010, 31 (2):285-290 (in Chinese).[10] Shao Shuang, Ma Boying, Wang Xuejie, et al. Study on the interaction between cefodizime sodium and bovine serum albumin [J]. Acta Phys. Chim. Sin. (物理化学学报), 2005, 21 (7):792-795 (in Chinese).[11] Gharagozlou M, Boghaei D M. Interaction of water-soluble amino acid Schiff base complexes with bovine serum albumin:fluorescence and circular dichroism studies [J]. Spectrochim. Acta, Part A, 2008, 71 (4):1617-1622.[12] Sukowska A, Bojko B, Rownicka J, et al. Paracetamol and cytarabine binding competition in high affinity binding sites of transporting protein [J]. J. Mol. Struct., 2006, 792-793 :249-256.[13] Sukowska A, Maci ek-Jurczyk M, Bojko B, et al. Competitive binding of phenylbutazone and colchicine to serum albumin in multidrug therapy [J]. J. Mol. Struct., 2008, 881 (1-3):97-106.[14] Maci ek-Jurczyk M, Sukowska A, Bojko B, et al. Fluorescence analysis of competition of phenylbutazone and methotrexate in binding to serum albumin in combination treatment in rheumatology [J]. J. Mol. Struct., 2009, 924-926 :378-384.[15] Liang Hong, Bian Hedong, Tu Chuqiao, et al. Binding equilibrium study between La(Ⅲ) and HAS or BSA [J]. Chem. J. Chin. Univ. (高等学校化学学报), 2001, 22 (1):21-25 (in Chinese).[16] Ilichev Y V, Perry J L, Simon J D, et al. Interaction of ochratoxin a with human serum albumin:preferential binding of the dianion and pH effects [J]. J. Phys. Chem. B, 2002, 106 (2):452-459.[17] Weiss S. Fluorescence spectroscopy of single biomolecules [J]. Science, 1999, 283 (5408):1676-1683.[18] Xu H, Gao S L, Lv J B, et al. Spectroscopic investigations on the mechanism of interaction of crystal violet with bovine serum albumin [J]. J. Mol. Struct., 2009, 919 (1-3):334-338.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

光谱探针猩红S与牛血清白蛋白结合的发光光谱

无机应力发光材料的发光特性、发光机理及应用研究进展

自激活LiSr₂Ca₂（BN₂）₃荧光粉的合成与发光性能

Pr³⁺掺杂红色长余辉发光材料研究进展

Sr₃(BN₂)₂荧光粉的合成与发光性能