稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用

冯小强; 伏国庆; 李小芳; 杨声; 苏中兴

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稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用

光谱分析 | 更新时间：2020-08-12

- 稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用
- Interaction of Rare Earth with Bovine Serum Albumin
- 发光学报 2011年32卷第2期页码：205-209
- 作者机构：
  
  1. 天水师范学院生命科学与化学学院,甘肃天水,741001
  2. 天水市中医医院,甘肃天水,741001
  3. 兰州大学化学化工学院,甘肃兰州,730030
- 作者简介：
- 基金信息：
  
  甘肃天水师范学院物理无机化学重点学科基金(ZD0840)资助项目()
- DOI：
  中图分类号： O657.3;O482.31
- 收稿日期：2010-06-04，
  
  修回日期：2010-08-10，
  
  网络出版日期：2011-02-22，
  
  纸质出版日期：2011-02-22
- 稿件说明：
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冯小强, 伏国庆, 李小芳, 杨声, 苏中兴. 稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用[J]. 发光学报, 2011,32(2): 205-209

FENG Xiao-qiang, FU Guo-qing, LI Xiao-fang, YANG Sheng, SU Zhong-xing. Interaction of Rare Earth with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(2): 205-209
冯小强, 伏国庆, 李小芳, 杨声, 苏中兴. 稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用[J]. 发光学报, 2011,32(2): 205-209 DOI：

FENG Xiao-qiang, FU Guo-qing, LI Xiao-fang, YANG Sheng, SU Zhong-xing. Interaction of Rare Earth with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(2): 205-209 DOI：

摘要

采用紫外吸收、荧光光谱和红外光谱

研究了稀土离子La(Ⅲ)和Nd(Ⅲ)与牛血清白蛋白(BSA)的相互作用。结果表明: La(Ⅲ)和Nd (Ⅲ)离子能配位诱导BSA

使其分子中色氨酸和酪氨酸芳杂环疏水基团外露

在280 nm处的吸收增强而表现出增色效应和较小的蓝移。La(Ⅲ)和Nd(Ⅲ)可以有规律地猝灭BSA的内源荧光

其猝灭机理是稀土离子与BSA形成复合物的静态猝灭。在20 ℃下

La(Ⅲ)和Nd (Ⅲ)与BSA的结合常数

分别为8.1210

和3.8510

。

Abstract

The interaction of rare earth La (Ⅲ) and Nd (Ⅲ) with BSA were studied by ultraviolet spectrum

fluorescence spectrum and IR spectrum. The results showed that the ultraviolet absorption intensity of BSA increases with the concentration of rare earth. Rare earth can strongly quench the fluorescence of BSA which is the static quenching. The binding constant (

) of La (Ⅲ) or Nd (Ⅲ) with BSA at room temperature are 8.1210

and 3.8510

respectively.

关键词

Keywords

references

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