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1. 郑州大学 化学系
2. 河南工业大学 化学化工学院, 河南 郑州 450052
收稿:2011-07-16,
修回:2011-9-11,
网络首发:2011-11-22,
纸质出版:2011-11-22
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于岚岚, 杨冉, 白希希, 陈晓英, 李建军, 屈凌波. 喜树碱与DNA相互作用的光谱学研究[J]. 发光学报, 2011,32(11): 1197-1203
YU Lan-lan, YANG Ran, BAI Xi-xi, CHEN Xiao-ying, LI Jian-jun, QU Ling-bo. Spectroscopic Study of The Interaction between Camptothecin and DNA[J]. Chinese Journal of Luminescence, 2011,32(11): 1197-1203
采用光谱法研究了抗癌药物喜树碱与DNA的相互作用。DNA引起喜树碱的吸收光谱轻微的红移和减色并导致喜树碱的荧光猝灭
说明喜树碱和DNA形成基态复合物。通过计算确定了二者的结合常数
结合位点数及主要结合力。同时考察了离子强度、磷酸根浓度、猝灭剂KI对二者相互作用的影响并比较了单、双链DNA和喜树碱相互作用的程度。变性实验确定了喜树碱-DNA复合物的熔点为60 ℃
比纯DNA的熔点低18 ℃。实验结果表明喜树碱和DNA之间主要是沟槽作用模式。
paper
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