H2O2胁迫下菠菜叶片延迟发光动力学过程的分析

习岗; 李少华; 张艳

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H₂O₂胁迫下菠菜叶片延迟发光动力学过程的分析

论文 | 更新时间：2020-08-12

- H₂O₂胁迫下菠菜叶片延迟发光动力学过程的分析
- Analysis of Delayed Luminescence Dynamic Processes of Spinach Leaves Stressed by H₂O₂
- 发光学报 2010年31卷第6期页码：952-956
- 作者机构：
  
  西安理工大学应用物理系,陕西西安,710048
- 作者简介：
- 基金信息：
  
  国家自然科学基金(50977079)();陕西省教育厅专项科研计划(09JK667)资助项目()
- DOI：
  中图分类号： Q632
- 收稿日期：2009-10-30，
  
  修回日期：2010-01-20，
  
  网络出版日期：2010-11-22，
  
  纸质出版日期：2010-11-22
- 稿件说明：
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习岗, 李少华, 张艳. H2O2胁迫下菠菜叶片延迟发光动力学过程的分析[J]. 发光学报, 2010,31(6): 952-956

XI Gang, LI Shao-hua, ZHANG Yan. Analysis of Delayed Luminescence Dynamic Processes of Spinach Leaves Stressed by H2O2[J]. Chinese Journal of Luminescence, 2010,31(6): 952-956
习岗, 李少华, 张艳. H2O2胁迫下菠菜叶片延迟发光动力学过程的分析[J]. 发光学报, 2010,31(6): 952-956 DOI：

XI Gang, LI Shao-hua, ZHANG Yan. Analysis of Delayed Luminescence Dynamic Processes of Spinach Leaves Stressed by H2O2[J]. Chinese Journal of Luminescence, 2010,31(6): 952-956 DOI：

摘要

从生物延迟发光的机理出发

建立了生物延迟发光动力学方程

发现菠菜叶片的延迟发光随时间按双指数规律衰减

表明菠菜叶片延迟发光来源于快项和慢项两个发光系统

其动力学行为可以通过发光动力学参数积分强度、初始光子数和衰变时间定量描述。用体积分数为10%的H

溶液处理菠菜叶片

随着处理时间的延长

延迟发光积分强度和初始光子数快速降低

积分强度和初始光子数的变化呈正相关

表征快相和慢相发光特征的两个衰变时间表现出逐渐减小的趋势。积分强度和初始光子数的变化代表了叶片的光合能力和代谢强度的下降

衰变时间的变化暗示H

可能对叶片PSⅡ中D1和D2两条多肽造成了破坏。

Abstract

Dynamic model of delayed luminescence of spinach leaves was proposed based on the mechanism of delayed luminescence in biological system. Delayed luminescence of spinach leaves reduced with time according to double-exponential law

it showed that delayed luminescence of spinach leaves comes from fast and slow items of light-emitting elements. The characteristics of delayed luminescence could be accurately described by parameters such as initial photon number

(0)

integrated intensity

I(T)

and decay time of delayed luminescence. Treated by solution of 10% H

I(T)

and

(0) decreased rapidly with treatment time

the changes of integrated intensity

I(T)

is proportional to changes of initial photon number

(0). Two decay times of the fast and slow items of light-emitting elements gradually decreased. The changes of

I(T)

and

(0) of delayed luminescence could represented decline in photosynthetic capacity and metabolic intensity of cell. The changes of decay times suggested that polypeptide D1 and D2 in PS Ⅱ of photosynthetic cells could be damaged by H

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references

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