氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记

封宾; 滕枫; 唐爱伟; 王琰; 侯延冰; 王永生

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氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记

论文 | 更新时间：2020-08-11

- 氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记
- Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme
- 发光学报 2007年28卷第3期页码：421-424
- 作者机构：
  
  北京交通大学, 光电子技术研究所, 发光与光信息技术教育部重点实验室, 北京, 100044
- 作者简介：
- 基金信息：
  
  国家“973”计划(2003CB314707)();国家自然科学基金(10434030,60677007,90401006)();北京市科技新星计划(2004B10)资
- DOI：
  中图分类号： O482.31
- 收稿日期：2007-01-14，
  
  修回日期：2007-02-24，
  
  纸质出版日期：2007-05-20
- 稿件说明：
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封宾, 滕枫, 唐爱伟, 王琰, 侯延冰, 王永生. 氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记[J]. 发光学报, 2007,28(3): 421-424,

FENG Bin, TENG Feng, TANG Ai-wei, WANG Yan, HOU Yan-bing, WANG Yong-sheng. Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme[J]. Chinese Journal of Luminescence, 2007,28(3): 421-424,
封宾, 滕枫, 唐爱伟, 王琰, 侯延冰, 王永生. 氨基酸稳定的CdSe纳米晶对溶菌酶进行荧光标记[J]. 发光学报, 2007,28(3): 421-424, DOI：

FENG Bin, TENG Feng, TANG Ai-wei, WANG Yan, HOU Yan-bing, WANG Yong-sheng. Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme[J]. Chinese Journal of Luminescence, 2007,28(3): 421-424, DOI：

摘要

利用氨基酸中惟一带有巯基的L-半胱氨酸盐酸盐(L-Cys)作为稳定剂在水相体系中合成CdSe纳米晶

通过表面包覆的L-Cys与生物试剂溶菌酶进行结合

实现CdSe纳米晶对溶菌酶的荧光标记.利用TEM、荧光光谱仪及荧光显微镜等多种手段对样品的结构、形貌及光学性能进行表征.试验结果表明

利用L-Cys为稳定剂合成的CdSe纳米晶具有较强的生物活性

L-Cys本身所带的氨基和羧基可以和多种生物试剂通过不同途径进行结合.对溶菌酶(Lys)标记前后

CdSe纳米晶的发射光谱的峰位发生微小红移

大约为2nm

半峰全宽基本保持不变

在40~50nm之间

荧光强度随所标记的Lys浓度的不同均发生明显增强.所有特性均表明实验所合成的CdSe纳米晶在生物标记方面具有很好的实用价值.

Abstract

Water-soluble CdSe nanocrystals were synthesized in the system of Na

SeSO

and Cd(CH

COO)

by using L-cysteine hydrochloride (L-Cys) as a stabilizer. Lysozyme (Lys) was labelled by the CdSe nanocrystals to investigate the application of CdSe nanocrystals as a fluorescent label which can be connected to the biologic molecule easily by the L-Cys over around the nanocrystals. There are more choice to label the biologic reagents because of the-NH

and-COOH of Lys. The powder of CdSe nanocrystals was characterized by transmission electron microscopy (TEM) and the optical properties was investigated by fluorescent spectral analysis and fluorescent microscope. It was proved that different CdSe nanocrystals with different sizes and optical pro-perties can be synthesized in different reaction condition. The peak wavelengthes of emission and absorption spectra of the CdSe nanocrystals are at about 530 and 410nm

respectively. After labeling

the full width at half maximum (FWHM) of CdSe nanocrystals can be kept well which was about 40nm and easy to distinguish during labeling the biologic reagents. Comparing with the optical properties before labelling

the emission maxima of the obtained CdSe nanocrystals have a little shift

just about 2nm

but the emission intensity of CdSe nanocrystals-labelled lysozyme can took a great enhancement about 100% with the change of the consistence of Lys. In sum

the L-cys-stabilized CdSe has an important value as a fluorescent label.

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references

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