To understand the interaction between adriamycin(ADR) and transferrin(Tf) solution

the binding reaction between ADR and Tf was investigated by fluorescence and ultraviolet visible Absorption(UV-Vis) spectroscopy methods.The mechanism of the fluorescence quenching of Tf induced by ADRwas determined.The relation between concentration of ADR and

F

0

/

F

was linear.It showed that the quenching of Tf induced by ADR was either dynamic or static quenching.The ultraviolet Absorption of Tf decreasing in the presence of ADR showed that a nonluminous compound was formed by the interaction of Tf and ADR.So it was confirmed that the mechanism of the fluorescence quenching of Tf induced by ADR was static quenching. Furthermore

we use Stern-Volmer equation to approve it.The equilibrium constants

K

were measured equaling to(0.98～89.1)×10

5

while the numbers of binding sites n were 1.09～1.37 at different concentration by means of fluorescence quenching method.The binding distance

r

=1.94 nm in the interaction of 6.9×10

-5

mol/L ADRand 5.6×10

-6

mol/L Tf was also obtained according to Frster theory of non-radiation energy transfer.Akind of binary compound of ADRand Tf was formed

providing the instructional meaning for the preparation of oriented drug carried by Tf.