By means of chemiluminescence analysis

the effects of birch sap bud its complex prescription on scavenging oxygen free radicals were investigated The superoxide dismutase (SOD) activity in animal blood and brain tissue

MAD(Malonaldehyde)content

immue function of PMN (Polymophonuciearleukocytes)

spontaneous luminescence of brain tissue

glutalthione peroxidase (GSH-PX)activity and hemoglobin(Hb)content in blood plasma were determined by hydroxylaminence method

thiobaribituric acid

cell chemiluminescence method

chemiluminescence

direct method and radioimmunoassay respectivily.The results showed that birch sap and its complex prescription had scavenging effects on O

2

-

OH

-

H

2

O

2

in vito.Using brain ischemia and reperfusion model

it is found that birch sap and its complex prescription could increase the activity of SOD and GSH-PX in blood and brain tissue;increase immunological competence of PMN cell;decrease content of LPO

TXB

2

and Hb in blood and brain tissue.It was the first time that the antioxydation of birch sap and its complex prescription was found.