Concanavalin A-induced chemiluminescence of the lymphocytes in sheep lung lymph was studied.The results indicate that determination of lymphocyte chemiluminescence has advantages of rapidity

simplicity and sensi-tivity and can be served as a method for determination of lymphocyte activity.The ideal determination system consisted of 1 ml of lymphocytic suspension(10

6

-10

7

cells/ml)

0.1 ml of Con-A solution(2mg/ml)and 50μl of luminol solution(10

-3

M).Polymorphonuclear leukocytes could enhance the chemiluminescence intensity of the system.So

purification of cells should not be ignored when the lymphocytes in peripheral blood were determined.Inhibition of the chemiluminescence by indomethacin suggested that the chemiluminescence of lymphocytes might related with the metabolism of arachidonic acid.