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中国科学院长春物理研究所
收稿:1987-11-28,
纸质出版:1989-02-28
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陈连春, 唐树延, 徐叙瑢. 凝聚相中叶绿素A荧光偏振光谱线型分析[J]. 发光学报, 1989,10(1): 54-81
Chen Lianchun, Tang Shuyan, Xu Xurong. THE SHAPES ANALYSIS FOR THE POLARIZED FLUORESCENCE OF CHLOROPHYLL A IN A CONDENSED STATE[J]. Chinese Journal of Luminescence, 1989,10(1): 54-81
本文报导了用氩离子激光器488nm线偏振光激发时叶绿素A(Chla)己烷溶液在12.5K下偏振荧光的实验结果。在波数为11200~20000cm
-1
范围内得到了完整的偏振荧光数据
并用计算机对仪器响应进行了校正。若把Chla单体、二聚体和齐聚体均视为荧光性杂质中心
则多中心荧光偏振度公式可写成:P(λ)=
<
img style="vertical-align: middle" alt="" order="0" src="http://www.rhhz.net/qikantupian/fgxb-19890109-54.gif" /
>
。用该公式进行了数值模拟计算
得到几种典型的P(λ)谱图
发现每一个高斯分量I
i
同P(λ)函数的一个峰
谷或肩呈现明显的对应关系。据此对chla的荧光偏振光谱的线型进行了分析和讨论。Chla单体、二聚体和齐聚体的不同激发态的荧光发射分量
均表现为P
λ
曲线上的峰、谷或肩。实验结果与数值模拟计算预期的线型完全一致。
This paper reports the study on the simulation of the shape of polarized spectra and on its application to analizing complex spectra. The 488nm-excited polarized fluorescence of chlorophyll a(Chla)solved in n-hexane is measured at 12.5K.New emission bands are observed.They are from the radiative transition of the higher excited state of Chla.The fluorescence spectrum of Chla is a superposition of several Gaussian components. Although a general theory that relates the polarized fluorescence intensities to the distribution of orientations
motion
local environments and energy transfer between the fluorescence molecules is very complicated
the spectra of Chla may still be very well explained by the results in Fig.l
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