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中国科学院长春物理研究所
纸质出版日期:1986-02-28
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陈连春, 唐树延, 徐叙瑢. 叶绿素A(Chla)溶液发光同浓度的关系[J]. 发光学报, 1986,7(1): 123-126
Chen lianchun, Tang Shuyan, Xu Xurong. RELATIONSHIP BETWEEN THE LUMINESCENCE AND CONCENTRATION OF CHLOROPHYLL A (chla) SOLUTION[J]. Chinese Journal of Luminescence, 1986,7(1): 123-126
我们在发光研究中
采用激发样品表层方法抑制自吸收—再发射的作用
得到较好结果
Chla己烷溶液和Chla乙醇溶液
在低浓度均有发光主峰683nm和次峰720nm
Chla己烷溶液发光在浓度为1×10
-5
M时开始浓度猝灭;Chla乙醇溶液发光
在5×10
-4
M时仍未见明显浓度猝灭。Chla己烷溶液发光
在不同浓度下
发光峰位基本不变
在高浓度尚有弱峰760nm。Chla己烷溶液发光主峰663nm随浓度增加向长波方向移动。
Many studies have been made on the luminescence of chla solution
but the results obtained were often influenced by different experimental conditions. In these
data is relatively difference re-absorption and re-emission of the sample on the luminescence of chla solution was discorvered by us
that influenced very much peaks and intensity of the luminesence in chla solution
but that was diminished by method excited surface layer of the sample. This paper report luminescences of chla solution in ethanol and n-hexane on the different concentration (see Table 1). The resultsshowed same peaks of the luminescence at 663nm and 720nm in chla solution of weeker concentration in ethanol and n-hexane. That appeared same centre of the luminescence excist in both of chla solution in ethanol and n-hexane chla solution in the n-hexane in the concentration of 1×10
-5
M showed the quenching of the luminescence. But chla solution in ethanol showed not apparent regard to concentration qunching of the luminescence (see Fig. 1). Concentration quenching of the luminescence was explaned by the exci plex of chla produced in chla solusion due to interaction of both between activated chla molecule and diactivated chla molecule (see Fig.2). Re-absorption and re emission of the luminescence in chla solution was measured
where different range of the sample was passed the luminescence due to different site of the sample was excited by Ar-laser 488nm. Peak of the luminescence on chla solution at 15000cm
-1
in 1×10
-7
M in ethanol shifed 50cm
-1
. So that re-absorption and re-emission was must noticed in the study on energe spectra of chla molecule.
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