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1. 中国科学院长春物理研究所
2. 白求恩医科大学
收稿日期:1984-06-16,
纸质出版日期:1985-11-30
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孟继武, 侯尚公, 唐树延, 徐叙瑢, 刘志广, 郑扶民. 人胃组织匀浆的荧光[J]. 发光学报, 1985,6(4): 341-347
Meng Jiwu, Hou Shanggong, Tang Shuyan, Xu Xurong, Liu Zhiguang, Zheng Fumin. THE INHERENT FLUORESCENCE OF THE HUMAN GASTRIC HOMOGENATE[J]. Chinese Journal of Luminescence, 1985,6(4): 341-347
本文报导了人胃组织匀浆的电子光谱。采用光谱解析法和分子轨道理论分析了匀浆中的发光中心。讨论了人胃组织匀浆的发光特征
并通过与人胃癌组织匀浆荧光光谱的对比分析
观察到了人胃癌组织匀浆的特异光谱。
Currently
methods of utilizing fluorescence in the diagnosis of cancers are roughly divided into two kinds: The method of fluorescence sensibilization and the method of direct observation of the inherent fluorescence. This paper reported the study of the electronic spectrum of the tissue homogenate of human stomach
and quantitatively investigated the center of luminescence. According to the observation of the inherent fluorescence
an early diagnosis of malignant tumors may be made preliminarily.The tissue homogenate of human stomach has a relatively strong photoabsorption in near ultraviolet and violet areas. The photoatosorption in violet area is the absorption of the purine compound in biologic tissues. It is evident that the electronic spectrum is influenced by the transition of the leading conjugated π electrons.It is obvious from the results of the experiment that utilizing ultraviolet light with different wave lengths to excite the gastric homogenate
roughly same integrated protein fluorescence emission peaks were obtained. Comparing this emission peak with that of the tryptophan (348nm)
some displacement was found
and this is just the fluorescence characteristic of the protein B. Therefore
it is concluded that under the excitation of ultraviolet light
the photospectrum of aromatic compound is the chief characteristic of that of the gastric homogenate.From the result of the experiment
it is noticed that the emission in red area of the ultraviolet-excited gastric homogenate was basically originated from the transition of the ρ electrons of purine cyclic group of hemoglobin. It possesses distinct common characteristics with ths fluorescence spectrum of the human whole blood
hemoglobin and hematoporphyrin. Therefore
the photoemission of gastric homogenate in visible area was chiefly originated from the hemoglobin molecules of the tissues.Once the pathologic lesions developed in tissues
it will inevitably lead to changes in the molecular composition
molecular structure as well as physicochemical environment around the emission center
eausing changes in the state of emission center as a result of changes in inherent fluorescence. In the comparison of the spectrum of inherent fluorescence in normal tissue with that in cancer tissues
it is obvious that there is significant difference between the emission peak of the fluorescence of cancer tissues and that of the normal tissue homogenate.
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